Screening and Identification of High-efficiency Phosphate Solubilizing Bacteria in Tobacco Rhizosphere and Its Growth-promoting Effects

doi:10.13560/j.cnki.biotech.bull.1985.2021-1511

Abstract

Abstract:

In order to obtain high-quality phosphate-solubilizing bacterial resources and enhance agricultural production efficiency,we screened phosphorus-solubilizing bacteria from the tobacco rhizosphere soil of Fangshi township,Gaomi city,Shandong province,among which the highly efficient phosphate-solubilizing bacterium 3P29 was identified by molecular biology,then the ability of phosphate-solubilizing and growth-promoting effect were further studied. The strain 3P29 was identified as Acinetobacter pittobacter and the transformation amount of lecithin and calcium phosphate were 13.38 mg/L and 19.83 mg/L,respectively,indicating that the strain 3P29 efficiently decomposed organic and inorganic phosphorus. Tobacco pot experiment showed that the primary root of tobacco became longer and more lateral roots were produced,thereby enhancing the absorption of nutrient elements by the roots. The total nitrogen,phosphorus and potassium content of tobacco leaves increased by 71%,49% and 134% under the conditions of phosphorus-free nutrient solution,which may provide superior strain resources for the development and utilization of phosphate solubilizing bacterial fertilizers.

Key words: phosphate solubilizing, root development, tobacco, Acinetobacter pittii

LIU Guang-chao, YE Qing, CHE yong-mei, LI Ya-hua, AN Dong, LIU Xin. Screening and Identification of High-efficiency Phosphate Solubilizing Bacteria in Tobacco Rhizosphere and Its Growth-promoting Effects[J]. Biotechnology Bulletin, 2022, 38(8): 179-187.

Figures/Tables 10

Table 1 Phosphorus solubilizing ability of strain 3P29

菌株编号 Strain No.	有机磷含量Organic phosphorus content /（mg·L^-1）	无机磷含量Inorganic phosphorus content /（mg·L^-1）
2P1	11.210	9.260
2P10	9.260	14.490
2P14	2.620	23.250
2P23	10.210	10.690
2P24	7.750	23.920
3P25	3.040	25.330
3P28	2.890	26.190
3P29	13.380	19.830
3P33	0.000	24.870
3P37	0.000	23.520

Fig.1 Colony morphology,Gram staining and auxin secretion ability of strain 3P29

Fig. 2 Phylogenetic tree established using the neighbor-joining method,based on 16S rDNA sequences of strain 3P29 and related strains

Fig. 3 Phylogenetic tree established using the neighbor-joining method,based on gyrB sequences of strain 3P29 and related strains

Fig. 4 Effects of strain 3P29 on mineral element contents in soil

Fig.5 Effects of strain 3P29 on soil enzyme activity

Fig. 6 Effects of strain 3P29 on tobacco growth CKt：Aseptic processing. CTt：Inoculation with 3P29. 1 represents phosphorus-free Hoagland nutrient solution culture,and 2 represents flower-free whole nutrient solution culture,bar=10 cm

Table 2 Effects of strain 3P29 on the growth index of tobacco

处理Treatment	株高Height/cm	叶面积Leaf area/cm²	叶片数Number of blades	茎粗Stem thick/cm
CKt1	10.70±0.40	304±0.42	6.67±0.58	0.83±0.03
CTt1	13.40±0.46**	370.67±0.55**	8.67±0.57**	1.13±0.06**
CKt2	12.23±0.25	342.67±0.15	8.00±0.35	1.03±0.15
CTt2	17.13±0.32**	489±0.49**	11.33±0.49**	1.33±0.03**

Table 3 Effects of strain 3P29 on the biomass of aerial and underground parts of tobacco

处理 Treatment	地上鲜重 Fresh weight on the ground/g	地上干重 Dry weight on the ground/g	地下鲜重 Underground fresh weight/g	地下干重 Underground dry weight/g
CKt1	34.37±0.42	3.12±0.03	1.30±0.10	0.12±0.01
CTt1	59.63±0.55**	6.05±0.05**	2.60±0.10**	0.27±0.01**
CKt2	53.83±0.15	5.45±0.04	1.80±0.10	0.18±0.01
CTt2	92.93±0.49**	9.46±0.02**	2.70±0.17**	0.29±0.01**

Fig. 7 Effects of strain 3P29 on contents of nitrogen,phosphorus and potassium in tobacco leaves **in the figure indicates significant differences at P<0.01 level

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