Molecular Evolutionary Relationship and Protein Structure of Glycoside Hydrolases from GH79 Family

doi:10.13560/j.cnki.biotech.bull.1985.2022-0417

Abstract

Abstract:

The glycoside hydrolases of the GH79 family have extensive physiological activities and important application prospects in carbohydrate modification, cellular immune recognition and signal transduction. However, the diverse catalysis mechanism of GH79 family remains unclear, and the structural basis and molecular mechanism of substrate recognition are still unclear. This review summarizes the research progress of the GH79 family, systematically analyzes the origin and distribution of GH79 family enzymes, and deeply expounds their sequence features, molecular evolutionary relationships, and the crystal structures analysis, aiming to lay a foundation for protein engineering and functional metabolic mechanism of GH79 family.

Key words: GH family 79, catalytic mechanism, molecular evolution, structural analysis

CHEN Quan-bing, CAO Wei-jie, LI Chun, LV Bo. Molecular Evolutionary Relationship and Protein Structure of Glycoside Hydrolases from GH79 Family[J]. Biotechnology Bulletin, 2023, 39(1): 104-114.

Figures/Tables 8

References 56

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水解方式 Hydrolysis method	名称 Name	切割位点 Cleavage site
外切型 Exo-type	唾液酸酶	α-2,6糖苷键连接的唾液酸残基
	β-半乳糖苷酶	β-1,4糖苷键连接的半乳糖残基
	α-甘露糖苷酶	α-1,2糖苷键，α-1,3糖苷键，α-1,6糖苷键连接的甘露糖型
	β-甘露糖苷酶	β-1,4糖苷键连接的甘露糖型
	β-淀粉酶	自非还原末端依次切割麦芽糖单位，不切也不逾越α-1,6糖苷键
内切型 Endo-type	α-淀粉酶	在分子内部随机切割α-1,4糖苷键，不切α-1,6糖苷键
	异淀粉酶	水解支链淀粉或糖原中的α-1,6糖苷键
	Endo F	β-1,4糖苷键连接的高甘露糖型杂合糖型
	Endo H	β-1,4糖苷键连接的高甘露糖型杂合糖型
	O-糖苷酶	水解蛋白核心1（Galβ1-3 GalNAC-Thr/Ser）和核心3（GlcNACβ1-3 GalNAC-Thr/Ser）之间的O-连接二糖单位

水解方式 Hydrolysis method	名称 Name	切割位点 Cleavage site
外切型 Exo-type	唾液酸酶	α-2,6糖苷键连接的唾液酸残基
	β-半乳糖苷酶	β-1,4糖苷键连接的半乳糖残基
	α-甘露糖苷酶	α-1,2糖苷键，α-1,3糖苷键，α-1,6糖苷键连接的甘露糖型
	β-甘露糖苷酶	β-1,4糖苷键连接的甘露糖型
	β-淀粉酶	自非还原末端依次切割麦芽糖单位，不切也不逾越α-1,6糖苷键
内切型 Endo-type	α-淀粉酶	在分子内部随机切割α-1,4糖苷键，不切α-1,6糖苷键
	异淀粉酶	水解支链淀粉或糖原中的α-1,6糖苷键
	Endo F	β-1,4糖苷键连接的高甘露糖型杂合糖型
	Endo H	β-1,4糖苷键连接的高甘露糖型杂合糖型
	O-糖苷酶	水解蛋白核心1（Galβ1-3 GalNAC-Thr/Ser）和核心3（GlcNACβ1-3 GalNAC-Thr/Ser）之间的O-连接二糖单位

蛋白名称 Protein name	来源 Organism	底物 Substrate	EC编号 EC#	参考文献 Reference
GlcA79A	Acidobacterium capsulatum	β-D-glucuronicacid	3.2.1.31	[13]
AtGUS2	Arabidopsis thaliana	pNPGlcA	3.2.1.31	[16]
Nc6GAL	Neurospora crassa	Arabinogalactan-proteins	3.2.1.31	[15]
FobglcA	Fusarium oxysporum	Gum arabic	3.2.1.31	[21]
BpHep	Burkholderia pseudomallei	Heparan sulfate	3.2.1.166	[14]
HpsE	Rattus norvegicus	Heparan sulfate	3.2.1.166	[18]
Hpse1	Danio rerio	Heparan sulfate glycosaminoglycans	3.2.1.166	[22]
Hpa1	Homo sapiens	Heparan sulfate	3.2.1.166	[19]
Heparanase	Gallus gallus	Heparan sulfate	3.2.1.166	[23]
Heparanase	Homo sapiens	Heparan sulfate	3.2.1.166	[24]
Heparanase	Sus scrofa	Heparan sulfate	3.2.1.166	[25]
Hpa	Mus musculus	Heparan sulfate	3.2.1.166	[17]
Hyaluronidase	Hirudo nipponia	Hyaluronan	3.2.1.36	[26]
LHyal	Hirudo nipponia	Hyaluronan	3.2.1.36	[27]
SguS	Scutellaria baicalensis	Baicalein-7-O-β-D-glucuronide-conjugated	3.2.1.167	[28]
AnGlcAase	Aspergillus niger	Arabinogalactan-proteins	3.2.1.-	[15]
TpGUS79A	Talaromyces pinophilus Li-93	Glycyrrhizinate	3.2.1.-	[29]
Glucuronidase 1	Prunus dulcis	Almond	3.2.1.-	[30]
Heparanase precursor	Bos taurus	Heparan sulfate	3.2.1.-	[31]
Hypothetical protein TrVGV298_008121	Trichoderma virens	-	3.2.1.-	[32]

蛋白名称 Protein name	来源 Organism	底物 Substrate	EC编号 EC#	参考文献 Reference
GlcA79A	Acidobacterium capsulatum	β-D-glucuronicacid	3.2.1.31	[13]
AtGUS2	Arabidopsis thaliana	pNPGlcA	3.2.1.31	[16]
Nc6GAL	Neurospora crassa	Arabinogalactan-proteins	3.2.1.31	[15]
FobglcA	Fusarium oxysporum	Gum arabic	3.2.1.31	[21]
BpHep	Burkholderia pseudomallei	Heparan sulfate	3.2.1.166	[14]
HpsE	Rattus norvegicus	Heparan sulfate	3.2.1.166	[18]
Hpse1	Danio rerio	Heparan sulfate glycosaminoglycans	3.2.1.166	[22]
Hpa1	Homo sapiens	Heparan sulfate	3.2.1.166	[19]
Heparanase	Gallus gallus	Heparan sulfate	3.2.1.166	[23]
Heparanase	Homo sapiens	Heparan sulfate	3.2.1.166	[24]
Heparanase	Sus scrofa	Heparan sulfate	3.2.1.166	[25]
Hpa	Mus musculus	Heparan sulfate	3.2.1.166	[17]
Hyaluronidase	Hirudo nipponia	Hyaluronan	3.2.1.36	[26]
LHyal	Hirudo nipponia	Hyaluronan	3.2.1.36	[27]
SguS	Scutellaria baicalensis	Baicalein-7-O-β-D-glucuronide-conjugated	3.2.1.167	[28]
AnGlcAase	Aspergillus niger	Arabinogalactan-proteins	3.2.1.-	[15]
TpGUS79A	Talaromyces pinophilus Li-93	Glycyrrhizinate	3.2.1.-	[29]
Glucuronidase 1	Prunus dulcis	Almond	3.2.1.-	[30]
Heparanase precursor	Bos taurus	Heparan sulfate	3.2.1.-	[31]
Hypothetical protein TrVGV298_008121	Trichoderma virens	-	3.2.1.-	[32]