The Cloning of cDNA of SLA-DRB from Hebao Pigs and the Analysis of Their Molecular Evolutionary Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.022

Abstract

Abstract:

In order to study the molecular characteristics of SLA-DRB derived from Hebao pigs, a pair of primers were designed to amplify the cDNA of SLA-DRB from 3 Hebao pigs. Then the amplified cDNA were cloned into pMD18-T vector and the positive clones were sequenced and analyzed by homology comparing, molecular evolution analysis, and comparing and analyzing the key amino acid sites. It was shown that the SLA-DRB alleles were amplified successfully from the tissues of the 3 Hebao pigs, and the alleles were designated as SLA-DRB-HB01, 02, and 03. After sequencing, the results showed that the cDNA of SLA-DRB-HB alleles was 836 bp, and the open reading fragments(ORF)of SLA-DRB-HB locating at sites of 1-801, encoding 266 amino acids. By homology analyzing, the SLA-DRB-HB genes had a homology value of 90.3%-99.8% with other SLA-DRB alleles. The analysis of molecular evolution showed that the SLA-DRB-HB alleles were clustered an independent branch of phylogenetic tree, and SLA-DRB-HB evolved more primarily in contrast to other SLA-DRB alleles. The analysis of the mutated amino acids sites and the polymorphism of SLA-DRB-HB demonstrated that they had polymorphism.

Key words: Hebao pig, SLA-DRB, molecular evolution, polymorphism

Jiang Ping, Erdemtu, Gao Fengshan. The Cloning of cDNA of SLA-DRB from Hebao Pigs and the Analysis of Their Molecular Evolutionary Characteristics[J]. Biotechnology Bulletin, 2015, 31(12): 150-157.

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