Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (10): 18-28.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1572

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Structure and Application of C-glycosyltransferases in Plants

ZHAO Yu-xue1(), WANG Yun2, YU Lu-yao3, LIU Jing-jing1, SI Jin-ping1, ZHANG Xin-feng1(), ZHANG Lei1,2,3()   

  1. 1. State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300
    2. Biomedical Innovation R&D Center,School of Medicine,Shanghai University,Shanghai 200444
    3. Department of Pharmaceutical Botany,School of Pharmacy,Naval Medical University,Shanghai 200433
  • Received:2021-12-20 Online:2022-10-26 Published:2022-11-11
  • Contact: ZHANG Xin-feng,ZHANG Lei E-mail:786330520@qq.com;zhangxf73@163.com;starzhanglei@aliyun.com

Abstract:

Glycosyltransferases are a class of post-modification enzymes that specifically catalyse glycosylation reactions and are classified into 4 types of glycosyltransferases,O-,C-,N- and S-,depending on the acceptor molecule attached to the sugar donor. In recent years,along with the vigorous development of structural biology,reports on the structural elucidation and targeted modification of C-glycosyltransferases have gradually increased,providing theoretical guidance for the modification of complex natural products. This paper reviews the research progress of plant C-glycosyltransferases,discusses them from the perspectives of their substrate specificity,spatial structure of sugar donor binding and biotechnological applications,and explores the structural basis of the substrate catalytic characteristics,which may provide a reference for the subsequent heterologous synthesis of plant natural products by means of synthetic biology.

Key words: C-glycosyltransferase, biosynthetic pathway, catalytic mechanism, crystal structure, synthetic biology