Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 40-47.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1227
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LI Peng-cheng(), ZHANG Ming-jun, WANG Yin-xiao, LI Xiang-yin, LI Sheng-yan(), LANG Zhi-hong()
Received:
2022-10-08
Online:
2023-01-26
Published:
2023-02-02
Contact:
LI Sheng-yan,LANG Zhi-hong
E-mail:13126701192@163.com;lishengyan@caas.cn;langzhihong@caas.cn
LI Peng-cheng, ZHANG Ming-jun, WANG Yin-xiao, LI Xiang-yin, LI Sheng-yan, LANG Zhi-hong. Insect Resistance Identification and Agronomy Traits Analysis of Transgenic Maize HGK60 with Different Genetic Backgrounds[J]. Biotechnology Bulletin, 2023, 39(1): 40-47.
Fig. 1 Detection of 4 transgenic inbred lines and 2 hybrid combinations using event-special PCR M: DNA marker; CK+ : positive control; CK-: negative control; CK-(blank): ddH2O as template; 1-3: HGK60-Zheng 58; 4-6: HGK60-Chang 7-2; 7-9: HGK60 lx03-2; 10-12: HGK60-lx05-4; 13-15: HGK60-Zhengdan 958; 16-18: HGK60-ludan 9066
玉米材料 Corn material | 2020年海南三亚 Sanya, Hainan in 2020 | 2021年河北廊坊 Langfang, Hebei in 2021 | 2021年海南三亚 Sanya, Hainan in 2022 | |||
---|---|---|---|---|---|---|
3 d死亡率 3-day mortality rate/% | 4 d死亡率 4-day mortality rate/% | 3 d死亡率 3-day mortality rate/% | 4 d死亡率 4-day mortality rate/% | 3 d死亡率 3-day mortality rate/% | 4 d死亡率 4-day mortality rate/% | |
HGK60-郑58 | 100.00±0.00 | - | 100.00±0.00 | - | 91.67±4.17 | 100.00±0.00 |
HGK60-昌7-2 | 100.00±0.00 | - | 100.00±0.00 | - | 93.06±2.41 | 100.00±0.00 |
HGK60-lx03-2 | 100.00±0.00 | - | 100.00±0.00 | - | 97.22±2.41 | 100.00±0.00 |
HGK60-lx05-4 | 100.00±0.00 | - | 100.00±0.00 | - | 94.44±2.41 | 100.00±0.00 |
HGK60-郑单958 | 100.00±0.00 | - | 100.00±0.00 | - | 94.44±2.41 | 100.00±0.00 |
HGK60-鲁单9066 | 100.00±0.00 | - | 100.00±0.00 | - | 98.61±2.41 | 100.00±0.00 |
非转基因玉米-郑58 | 1.39±2.41 | 1.39±2.41 | 0.00±0.00 | 0.00±0.00 | 5.56±2.41 | 5.56±2.41 |
Table 1 Results of laboratory bioassay of Asian corn borer(Ostrinia furnacalis)
玉米材料 Corn material | 2020年海南三亚 Sanya, Hainan in 2020 | 2021年河北廊坊 Langfang, Hebei in 2021 | 2021年海南三亚 Sanya, Hainan in 2022 | |||
---|---|---|---|---|---|---|
3 d死亡率 3-day mortality rate/% | 4 d死亡率 4-day mortality rate/% | 3 d死亡率 3-day mortality rate/% | 4 d死亡率 4-day mortality rate/% | 3 d死亡率 3-day mortality rate/% | 4 d死亡率 4-day mortality rate/% | |
HGK60-郑58 | 100.00±0.00 | - | 100.00±0.00 | - | 91.67±4.17 | 100.00±0.00 |
HGK60-昌7-2 | 100.00±0.00 | - | 100.00±0.00 | - | 93.06±2.41 | 100.00±0.00 |
HGK60-lx03-2 | 100.00±0.00 | - | 100.00±0.00 | - | 97.22±2.41 | 100.00±0.00 |
HGK60-lx05-4 | 100.00±0.00 | - | 100.00±0.00 | - | 94.44±2.41 | 100.00±0.00 |
HGK60-郑单958 | 100.00±0.00 | - | 100.00±0.00 | - | 94.44±2.41 | 100.00±0.00 |
HGK60-鲁单9066 | 100.00±0.00 | - | 100.00±0.00 | - | 98.61±2.41 | 100.00±0.00 |
非转基因玉米-郑58 | 1.39±2.41 | 1.39±2.41 | 0.00±0.00 | 0.00±0.00 | 5.56±2.41 | 5.56±2.41 |
年代地点 Year and location | 转基因玉米 Transgenic maize | 抗性等级 Resistance level | 非转基因玉米 Non-transgenic maize | 抗性等级 Resistance level |
---|---|---|---|---|
2020年海南三亚 | HGK60-郑58 | 1.34±0.38a | 郑58 | 7.03±0.23b |
HGK60-昌7-2 | 1.31±0.57a | 昌7-2 | 6.34±0.23b | |
HGK60-lx03-2 | 1.48±0.35a | lx03-2 | 6.48±0.78b | |
HGK60-lx05-4 | 1.72±0.31a | lx05-4 | 6.95±0.49b | |
HGK60-郑单958 | 1.23±0.94a | 郑单958 | 6.67±0.69b | |
HGK60-鲁单9066 | 1.28±0.46a | 鲁单9066 | 6.89±0.87b | |
2021年河北廊坊 | HGK60-郑58 | 1.39±0.45a | 郑58 | 7.56±0.49b |
HGK60-昌7-2 | 1.78±0.30a | 昌7-2 | 6.63±0.71b | |
HGK60-lx03-2 | 1.48±0.45a | lx03-2 | 6.93±0.59b | |
HGK60-lx05-4 | 1.42±0.40a | lx05-4 | 7.01±0.52b | |
HGK60-郑单958 | 1.31±0.29a | 郑单958 | 6.85±0.27b | |
HGK60-鲁单9066 | 1.37±0.65a | 鲁单9066 | 6.42±0.68b | |
2021年海南三亚 | HGK60-郑58 | 1.21±0.71a | 郑58 | 6.21±0.18b |
HGK60-昌7-2 | 1.51±0.54a | 昌7-2 | 6.78±0.56b | |
HGK60-lx03-2 | 1.63±0.34a | lx03-2 | 6.97±0.72b | |
HGK60-lx05-4 | 1.51±0.11a | lx05-4 | 7.31±0.67b | |
HGK60-郑单958 | 1.18±0.54a | 郑单958 | 6.41±0.93b | |
HGK60-鲁单9066 | 1.23±0.43a | 鲁单9066 | 6.56±0.67b |
Table 2 Leaf feeding grades of the Asian corn borer in the maize small trumpet stage
年代地点 Year and location | 转基因玉米 Transgenic maize | 抗性等级 Resistance level | 非转基因玉米 Non-transgenic maize | 抗性等级 Resistance level |
---|---|---|---|---|
2020年海南三亚 | HGK60-郑58 | 1.34±0.38a | 郑58 | 7.03±0.23b |
HGK60-昌7-2 | 1.31±0.57a | 昌7-2 | 6.34±0.23b | |
HGK60-lx03-2 | 1.48±0.35a | lx03-2 | 6.48±0.78b | |
HGK60-lx05-4 | 1.72±0.31a | lx05-4 | 6.95±0.49b | |
HGK60-郑单958 | 1.23±0.94a | 郑单958 | 6.67±0.69b | |
HGK60-鲁单9066 | 1.28±0.46a | 鲁单9066 | 6.89±0.87b | |
2021年河北廊坊 | HGK60-郑58 | 1.39±0.45a | 郑58 | 7.56±0.49b |
HGK60-昌7-2 | 1.78±0.30a | 昌7-2 | 6.63±0.71b | |
HGK60-lx03-2 | 1.48±0.45a | lx03-2 | 6.93±0.59b | |
HGK60-lx05-4 | 1.42±0.40a | lx05-4 | 7.01±0.52b | |
HGK60-郑单958 | 1.31±0.29a | 郑单958 | 6.85±0.27b | |
HGK60-鲁单9066 | 1.37±0.65a | 鲁单9066 | 6.42±0.68b | |
2021年海南三亚 | HGK60-郑58 | 1.21±0.71a | 郑58 | 6.21±0.18b |
HGK60-昌7-2 | 1.51±0.54a | 昌7-2 | 6.78±0.56b | |
HGK60-lx03-2 | 1.63±0.34a | lx03-2 | 6.97±0.72b | |
HGK60-lx05-4 | 1.51±0.11a | lx05-4 | 7.31±0.67b | |
HGK60-郑单958 | 1.18±0.54a | 郑单958 | 6.41±0.93b | |
HGK60-鲁单9066 | 1.23±0.43a | 鲁单9066 | 6.56±0.67b |
年代地点 Year and location | 农艺性状 Agronomy traits | HGK60-郑单958 HGK60-zhengdan 958 | 郑单958 Zhengdan 958 | HGK60-鲁单9066 HGK60-Ludan 9066 | 鲁单9066 Ludan 9066 |
---|---|---|---|---|---|
2020年海南三亚 | 株高 | 190.34±12.31a | 193.13±11.71a | 223.97±10.13a | 228.14±12.15a |
穗位高 | 90.44±5.87a | 92.15±6.87a | 101.08±7.81a | 103.78±11.57a | |
穗长 | 16.01±0.85a | 15.95±1.06a | 14.91±1.75a | 14.13±1.48a | |
穗粗 | 4.43±0.25a | 4.77±0.22a | 3.78±0.28a | 3.78±0.22a | |
穗行数 | 13.95±1.45a | 14.98±1.25a | 12.87±1.01a | 12.00±0.91a | |
秃尖长 | 0.42±0.31a | 0.44±0.33a | 0.44±0.25a | 0.46±0.37a | |
行粒数 | 38.64±3.43a | 38.21±2.23a | 39.84±4.75a | 38.85±4.52a | |
百粒重 | 27.13±2.22a | 26.24±3.24a | 22.41±1.83a | 23.22±2.33a | |
单株产量 | 152.74±7.13a | 150.82±12.81a | 112.65±13.04a | 115.84±12.91a | |
2021年河北廊坊 | 株高 | 248.04±10.87a | 250.72±10.96a | 294.77±11.14a | 288.28±12.64a |
穗位高 | 119.51±9.04a | 120.61±9.15a | 133.34±7.47a | 137.18±9.92a | |
穗长 | 19.81±1.22a | 18.94±1.41a | 21.64±2.33a | 22.05±0.87a | |
穗粗 | 4.85±0.32a | 4.81±0.26a | 4.65±0.24a | 4.65±0.16a | |
穗行数 | 15.62±1.27a | 15.81±1.47a | 13.76±1.21a | 13.85±1.56a | |
秃尖长 | 0.56±0.36a | 0.65±0.46a | 0.47±0.47a | 0.75±0.56a | |
行粒数 | 39.71±3.15a | 39.63±3.96a | 41.35±6.76a | 42.86±2.55a | |
百粒重 | 34.85±1.34a | 35.23±1.55a | 29.51±1.16a | 29.91±1.35a | |
单株产量 | 188.51±12.35a | 191.61±13.26a | 161.57±10.1a | 164.26±12.63a | |
2021年海南三亚 | 株高 | 187.34±11.23a | 188.24±13.42a | 224.01±11.24a | 226.89±11.26a |
穗位高 | 88.43±4.67a | 90.10±5.65a | 99.08±6.19a | 101.68±12.68a | |
穗长 | 15.93±0.45a | 16.02±1.23a | 14.92±2.03a | 14.61±1.39a | |
穗粗 | 4.35±0.21a | 4.43±0.18a | 3.56±0.30a | 3.61±0.32a | |
穗行数 | 14.01±1.14a | 14.83±1.34a | 12.73±1.34a | 12.34±0.56a | |
秃尖长 | 0.40±0.24a | 0.45±0.38a | 0.45±0.34a | 0.46±0.35a | |
行粒数 | 37.94±2.54a | 38.45±3.12a | 38.45±4.67a | 39.05±4.52a | |
百粒重 | 26.63±1.39a | 25.93±2.18a | 23.02±1.75a | 24.01±2.61a | |
单株产量 | 155.53±6.19a | 153.54±11.67a | 113.43±12.07a | 114.45±13.45a |
Table 3 Agronomic traits analysis of transgenic maize hybrid lines
年代地点 Year and location | 农艺性状 Agronomy traits | HGK60-郑单958 HGK60-zhengdan 958 | 郑单958 Zhengdan 958 | HGK60-鲁单9066 HGK60-Ludan 9066 | 鲁单9066 Ludan 9066 |
---|---|---|---|---|---|
2020年海南三亚 | 株高 | 190.34±12.31a | 193.13±11.71a | 223.97±10.13a | 228.14±12.15a |
穗位高 | 90.44±5.87a | 92.15±6.87a | 101.08±7.81a | 103.78±11.57a | |
穗长 | 16.01±0.85a | 15.95±1.06a | 14.91±1.75a | 14.13±1.48a | |
穗粗 | 4.43±0.25a | 4.77±0.22a | 3.78±0.28a | 3.78±0.22a | |
穗行数 | 13.95±1.45a | 14.98±1.25a | 12.87±1.01a | 12.00±0.91a | |
秃尖长 | 0.42±0.31a | 0.44±0.33a | 0.44±0.25a | 0.46±0.37a | |
行粒数 | 38.64±3.43a | 38.21±2.23a | 39.84±4.75a | 38.85±4.52a | |
百粒重 | 27.13±2.22a | 26.24±3.24a | 22.41±1.83a | 23.22±2.33a | |
单株产量 | 152.74±7.13a | 150.82±12.81a | 112.65±13.04a | 115.84±12.91a | |
2021年河北廊坊 | 株高 | 248.04±10.87a | 250.72±10.96a | 294.77±11.14a | 288.28±12.64a |
穗位高 | 119.51±9.04a | 120.61±9.15a | 133.34±7.47a | 137.18±9.92a | |
穗长 | 19.81±1.22a | 18.94±1.41a | 21.64±2.33a | 22.05±0.87a | |
穗粗 | 4.85±0.32a | 4.81±0.26a | 4.65±0.24a | 4.65±0.16a | |
穗行数 | 15.62±1.27a | 15.81±1.47a | 13.76±1.21a | 13.85±1.56a | |
秃尖长 | 0.56±0.36a | 0.65±0.46a | 0.47±0.47a | 0.75±0.56a | |
行粒数 | 39.71±3.15a | 39.63±3.96a | 41.35±6.76a | 42.86±2.55a | |
百粒重 | 34.85±1.34a | 35.23±1.55a | 29.51±1.16a | 29.91±1.35a | |
单株产量 | 188.51±12.35a | 191.61±13.26a | 161.57±10.1a | 164.26±12.63a | |
2021年海南三亚 | 株高 | 187.34±11.23a | 188.24±13.42a | 224.01±11.24a | 226.89±11.26a |
穗位高 | 88.43±4.67a | 90.10±5.65a | 99.08±6.19a | 101.68±12.68a | |
穗长 | 15.93±0.45a | 16.02±1.23a | 14.92±2.03a | 14.61±1.39a | |
穗粗 | 4.35±0.21a | 4.43±0.18a | 3.56±0.30a | 3.61±0.32a | |
穗行数 | 14.01±1.14a | 14.83±1.34a | 12.73±1.34a | 12.34±0.56a | |
秃尖长 | 0.40±0.24a | 0.45±0.38a | 0.45±0.34a | 0.46±0.35a | |
行粒数 | 37.94±2.54a | 38.45±3.12a | 38.45±4.67a | 39.05±4.52a | |
百粒重 | 26.63±1.39a | 25.93±2.18a | 23.02±1.75a | 24.01±2.61a | |
单株产量 | 155.53±6.19a | 153.54±11.67a | 113.43±12.07a | 114.45±13.45a |
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