Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (10): 323-331.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0291
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WANG Xin-yi1(), WANG Xiao-qian2, WANG Hong-jun2, CHAO Yue-hui1()
Received:
2023-03-30
Online:
2023-10-26
Published:
2023-11-28
Contact:
CHAO Yue-hui
E-mail:nqzxwxy@163.com;chaoyuehui@bjfu.edu.cn
WANG Xin-yi, WANG Xiao-qian, WANG Hong-jun, CHAO Yue-hui. Screening, Expression, and Validation of Nanobodies with FLAG Tag[J]. Biotechnology Bulletin, 2023, 39(10): 323-331.
引物名称 Primer name | 序列 Sequence(5'-3') |
---|---|
pGBKT7-FLAG-F | GGAGGCCGAATTCCCGGACTATAAAGACGACGACGATAAAGGGATCCGTCGACCT |
pGBKT7-FLAG-R | AGGTCGACGGATCCCTTTATCGTCGTCGTCTTTATAGTCCGGGAATTCGGCCTCC |
T7 promoter | AATACGACTCACTATAGGGC |
3'BD | TTTTCGTTTTAAAACCTAAGAGTC |
3'AD | AGATGGTGCACGATGCACAG |
pCold-VHH-F | GATTACGCTCATATGGCCATGGAGGCCAGTCAGGTTCAGCTGCAGGAGTCTGGRGGAGG |
pCold-VHH-R | GTTTTTCAGTATCTACGATTCATCTGCAGCTTACGCAGAAGAGACGGTGACCWGGGT |
pCold-F | AAACCACTCCACTGCGTCGTCTG |
pCold-R | CAGGGATCTTAGATTCTGTG |
Table 1 Primer sequences used in the experiment
引物名称 Primer name | 序列 Sequence(5'-3') |
---|---|
pGBKT7-FLAG-F | GGAGGCCGAATTCCCGGACTATAAAGACGACGACGATAAAGGGATCCGTCGACCT |
pGBKT7-FLAG-R | AGGTCGACGGATCCCTTTATCGTCGTCGTCTTTATAGTCCGGGAATTCGGCCTCC |
T7 promoter | AATACGACTCACTATAGGGC |
3'BD | TTTTCGTTTTAAAACCTAAGAGTC |
3'AD | AGATGGTGCACGATGCACAG |
pCold-VHH-F | GATTACGCTCATATGGCCATGGAGGCCAGTCAGGTTCAGCTGCAGGAGTCTGGRGGAGG |
pCold-VHH-R | GTTTTTCAGTATCTACGATTCATCTGCAGCTTACGCAGAAGAGACGGTGACCWGGGT |
pCold-F | AAACCACTCCACTGCGTCGTCTG |
pCold-R | CAGGGATCTTAGATTCTGTG |
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