Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (2): 147-160.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0746

Previous Articles     Next Articles

Genome-wide Identification of XTH Gene Family and Their Interacting miRNAs and Possible Roles in Low Temperature Adaptation in Jatropha curcas L.

LV Yu-jing1(), WU Dan-dan1, KONG Chun-yan1,2, YANG Yu1, GONG Ming1()   

  1. 1. School of Life Sciences, Yunnan Normal University, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy of Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Kunming 650500
    2. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Honghe 661699
  • Received:2022-06-21 Online:2023-02-26 Published:2023-03-07

Abstract:

Jatropha curcas L. has been widely considered as a promising and multi-purpose energy plant, also a kind of thermophilic chilling-sensitive plants, and low temperature severely affects their growth, development and utilization. It is known that xyloglucan endotransglucosylase/hydrolases(XTH)act as cell wall modifying enzymes and are involved in response and adaptation of plants to stresses. The previous work showed that chill-hardening at 12℃ significantly enhanced chilling resistance of J. curcas plants, and XTH genes demonstrated high-response to the chill-hardening. In the present study, genome-wide identification, bioinformatics analysis, expression profile analysis of XTH gene family, and identification and analysis of their interacting miRNAs were performed based on the obtained data of transcriptome, miRNAome and degradome of J. curcas during the chill-hardening. The results showed that 29 members of the XTH gene family were identified in J. curcas, encoding 269 to 341 amino acids, their proteins were mainly located to cell walls, and contained Glyco_hyho_16 and XET_C domains and the conserved site ExDxE. The 29 members of JcXTH family could be divided into three groups, located on nine chromosomes, and there were 8 pairs of gene duplication events. Expression profiling and RT-qPCR analysis demonstrated there were significantly differential expression patterns in different organs of J. curcas plants and under chilling-hardening, drought and salt stresses, implying that there were differential roles in various members of the XTH gene family. Analysis of sequencing data of miRNAome and degradome revealed that 68 miRNAs were found to target 26 members of JcXTH family, and analysis of expression profile and their correlation of these miRNAs and the targeting JcXTH genes highly responsive to the chill-hardening demonstrated a totally negatively regulated relationship, indicating these miRNAs were involved in a negative regulation of JcXTH gene expression. These results will be conducive to elucidating the functions of JcXTH gene family and how the interaction between the miRNAs and the JcXTH genes targeted to was involved in adaptation of J. curcas to low temperature stress, which will provide useful reference for further molecular breeding to improve chilling resistance in J. curcas.

Key words: Jatropha curcas, XTH gene family, miRNAs, interaction, low temperature adaptation