Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (2): 161-171.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0500
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YAO Xiao-wen1,2,3(), LIANG Xiao2,3, CHEN Qing2,3(), WU Chun-ling2,3, LIU Ying2,3, LIU Xiao-qiang2,3, SHUI Jun1,2,3, QIAO Yang1,2,3, MAO Yi-ming1, CHEN Yin-hua1, ZHANG Yin-dong1
Received:
2022-04-22
Online:
2023-02-26
Published:
2023-03-07
YAO Xiao-wen, LIANG Xiao, CHEN Qing, WU Chun-ling, LIU Ying, LIU Xiao-qiang, SHUI Jun, QIAO Yang, MAO Yi-ming, CHEN Yin-hua, ZHANG Yin-dong. Study on the Expression Pattern of Genes in Lignin Biosynthesis Pathway of Cassava Resisting to Tetranychus urticae[J]. Biotechnology Bulletin, 2023, 39(2): 161-171.
Gene name | Gene ID | Primer sequence(5'-3') | Tm/℃ | Product size/bp |
---|---|---|---|---|
PAL | MANES_08G008400 | F: AGGCATTTGGAGGAGAACTTGA R: TCGCAGAATCTTGATGGGTGA | 61.2 59.8 | 113 |
C4H | MANES_18G126900 | F: GCTCGCCAACAATCCTGCTC R: CTCCTCCTTCCAACTCCAAACG | 58.7 59.3 | 158 |
4CL | MANES_17G006400 | F: GGACTGTTGTCACTGGGCACTA R: TCTTCGTCAACGCTTGGAGTA | 59.9 58.5 | 133 |
CCR | MANES_04G103200 | F: GTCAATGCTGTTCAAGGCTATGTG R: AAATGTATCGCCCGGAGGC | 61.6 62.2 | 94 |
CAD | MANES_13G117800 | F: ACCAGAGGGAATGTCACCAGAA R: CCCTACTCCTCCAAGCCCTAAT | 60.8 60.9 | 133 |
HCT | MANES_04G101700 | F: GACCTTGTGGTGCCGAGATTC R: CGTCCAGCCATCGGATAGAAC | 61.7 61.9 | 137 |
CSE | MANES_12G157800 | F: TCCAATCGGAACCCAACACG R: AAGAGCAGCCCATACACGAAGAG | 63.6 62.7 | 112 |
CCoAOMT | MANES_10G078800 | F: GCCCACATCAATGGCTTCC R: GCTCCTTCATAGGTTCAGGCTCT | 60.7 61.1 | 152 |
COMT | MANES_01G043700 | F: GGCTGACCACTCAACCATTACC R: GACCGCTCCAGTTCCACCA | 60.9 61.2 | 103 |
F5H | MANES_04G084300 | F: GGTGGCATCGGCAATAGAGTG R: TGCAAGAGGAGTGGGATAGGTG | 62.7 61.6 | 198 |
Table 1 Candidate gene primers in the synthesis pathways of cassava(Manihot esculenta Crantz)lignin
Gene name | Gene ID | Primer sequence(5'-3') | Tm/℃ | Product size/bp |
---|---|---|---|---|
PAL | MANES_08G008400 | F: AGGCATTTGGAGGAGAACTTGA R: TCGCAGAATCTTGATGGGTGA | 61.2 59.8 | 113 |
C4H | MANES_18G126900 | F: GCTCGCCAACAATCCTGCTC R: CTCCTCCTTCCAACTCCAAACG | 58.7 59.3 | 158 |
4CL | MANES_17G006400 | F: GGACTGTTGTCACTGGGCACTA R: TCTTCGTCAACGCTTGGAGTA | 59.9 58.5 | 133 |
CCR | MANES_04G103200 | F: GTCAATGCTGTTCAAGGCTATGTG R: AAATGTATCGCCCGGAGGC | 61.6 62.2 | 94 |
CAD | MANES_13G117800 | F: ACCAGAGGGAATGTCACCAGAA R: CCCTACTCCTCCAAGCCCTAAT | 60.8 60.9 | 133 |
HCT | MANES_04G101700 | F: GACCTTGTGGTGCCGAGATTC R: CGTCCAGCCATCGGATAGAAC | 61.7 61.9 | 137 |
CSE | MANES_12G157800 | F: TCCAATCGGAACCCAACACG R: AAGAGCAGCCCATACACGAAGAG | 63.6 62.7 | 112 |
CCoAOMT | MANES_10G078800 | F: GCCCACATCAATGGCTTCC R: GCTCCTTCATAGGTTCAGGCTCT | 60.7 61.1 | 152 |
COMT | MANES_01G043700 | F: GGCTGACCACTCAACCATTACC R: GACCGCTCCAGTTCCACCA | 60.9 61.2 | 103 |
F5H | MANES_04G084300 | F: GGTGGCATCGGCAATAGAGTG R: TGCAAGAGGAGTGGGATAGGTG | 62.7 61.6 | 198 |
Fig. 1 Changes of PAL gene expressions in different varieties at different times before and after mite infestation A: Changes of gene expressions in various varieties before and after mite infestation. B: Changes of gene expressions between varieties at the same time before and after mite infestation. Different lowercase letters indicate that the expression amount after different time of mite infested is significantly different from that before mite infested(P<0.05). The same below
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