Efficient Generation of Secondary Libraries During Functional Nucleic Acids Screening

doi:10.13560/j.cnki.biotech.bull.1985.2022-0743

Abstract

Abstract:

In vitro screening of functional nucleic acids（FNAs）by SELEX is an essential prerequisite for their various functions. In the SELEX process, the preparation of high-quality single-strand secondary library is one of the most basic and critical technologies, which largely determines the success or failure of screening. Here, we comprehensively summarize the generation strategies of single-strand DNA for FNAs selection, such as amplification-based methods, enzymatic digestion techniques, magnetic separation with streptavidin-coated beads, and secondary library regeneration strategies based on mobility shift. The advantages, disadvantages and key details of various methods are elaborated with aiming to lay a foundation for the further development of efficient FNAs screening methods.

Key words: functional nucleic acids, screening, aptamer, PCR, generation of ssDNA

LI Tian-shun, LI Chen-wei, WANG Jia, ZHU Long-Jiao, XU Wen-tao. Efficient Generation of Secondary Libraries During Functional Nucleic Acids Screening[J]. Biotechnology Bulletin, 2023, 39(3): 116-122.

Figures/Tables 1

References 47

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