Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (5): 224-232.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1285

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Heterologous Expression of Soybean Transcription Factor GmNF-YA19 Improves Drought Resistance of Transgenic Tobacco

ZHAI Ying1(), LI Ming-yang1, ZHANG Jun2, ZHAO Xu3, YU Hai-wei1, LI Shan-shan1, ZHAO Yan1, ZHANG Mei-juan1, SUN Tian-guo1   

  1. 1. College of Life Science and Agroforestry, Qiqihar University, Qiqihar 161006
    2. Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005
    3. Jilin Province Institute of Product Quality Supervision and Inspection,Changchun 130022
  • Received:2022-10-18 Online:2023-05-26 Published:2023-06-08

Abstract:

Nuclear factor(NF-Y)is a kind of transcription factor widely existing in eukaryotes, and can play an important role in the regulation of abiotic stress response in plants. Exploring the drought resistance and mechanism of soybean GmNF-YA19 will lay a foundation for the application of GmNF-YA19 in drought resistant breeding. Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the expression of GmNF-YA19 under abiotic stress. GmNF-YA19 was cloned. Its plant expression vector was constructed and transformed into tobacco, and the drought resistance of transgenic tobacco was identified. RT-qPCR results showed that GmNF-YA19 responded to drought, salt, cold and exogenous ABA in soybean, and the expression of GmNF-YA19 increased most significantly under drought stress. GmNF-YA19 was cloned by PCR using soybean leaf cDNA as template. Sequence analysis revealed that GmNF-YA19 encoded a protein containing 213 amino acids, with predicted molecular weight of 22.99 kD and predicted isoelectric point of 9.40. GmNF-YA19 protein sequence contained a conserved CBF domain. Phylogenetic analysis demonstrated that GmNF-YA19 protein was closely related to OsNF-YA7, AtNF-YA4 and AtNF-YA7 proteins. Four transgenic tobacco plants were obtained. The heterologous expression of GmNF-YA19 enhanced the drought resistance of transgenic tobacco plant under drought stress. Compared with wild-type tobacco, the expressions of three stress-related genes(NtABA2, NtNCED3-2 and NtP5CS)in GmNF-YA19 transgenic tobacco significantly increased.

Key words: soybean, nuclear factor, NF-YA, abiotic stress, drought resistance