Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (7): 131-142.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0015

Previous Articles     Next Articles

Functional Analysis of Soybean Gene GmHMGR Responding to Exogenous Hormones and Abiotic Stresses

WANG Shuai1(), FENG Yu-mei2, BAI Miao1, DU Wei-jun1, YUE Ai-qin1()   

  1. 1. College of Agriculture, Shanxi Agricultural University, Taigu 030801
    2. College of Forestry, Shanxi Agricultural University, Taigu 030801
  • Received:2023-01-09 Online:2023-07-26 Published:2023-08-17
  • Contact: YUE Ai-qin E-mail:15234570975@163.com;yueaiqinnd@126.com

Abstract:

GmHMGR gene is one of the key enzyme genes in the mevalonate pathway of soybean(Glycine max L.), and plays an important role in the stress regulation. Exploring the function of the GmHMGR gene in soybean stress resistance will serve as a foundation for the analysis of soybean antioxidant, salt resistance mechanisms, and molecular breeding. The function of the GmHMGR gene under abiotic stress and hormone treatments was analyzed using real-time quantitative PCR, yeast transformation, and A. thaliana genetic transformation. The results showed that the GmHMGR gene responded to PEG6000, NaCl, H2O2, MeJA and ABA treatments. Overexpressing GmHMGR1, GmHMGR3, GmHMGR5, GmHMGR6 and GmHMGR7 genes improved yeast's salt resistance and antioxidant capacity. Overexpressing GmHMGR4 and GmHMGR6 genes in A. thaliana upregulated the expression levels of terpenoids metabolism-related enzymes genes(including AtDXR, AtSQS1, AtSQS2 and AtCAS), and improved its resistance capacity under oxidative and salt stress. GmHMGR gene may be involved in response to oxidative and salt stresses in soybean.

Key words: soybean, GmHMGR, heterologous expression, oxidative stress, salt stress, exogenous hormones