Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (7): 91-104.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1265
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YOU Zi-juan1(), CHEN Han-lin2, DENG Fu-cai2()
Received:
2022-10-12
Online:
2023-07-26
Published:
2023-08-17
Contact:
DENG Fu-cai
E-mail:youzijuan@163.com;dfcrndfcrn@163.com
YOU Zi-juan, CHEN Han-lin, DENG Fu-cai. Research Progress in the Extraction and Functional Activities of Bioactive Peptides from Fish Skin[J]. Biotechnology Bulletin, 2023, 39(7): 91-104.
常规提取方 Conventional extraction method | 优点 Advantages | 缺点 Disadvantages | 文献来源 Reference |
---|---|---|---|
酶法 Enzymatic method | 水解条件温和、反应安全可控,产物结构稳定,不破坏氨基酸,功能活性高,利于人体吸收 The hydrolysis conditions are mild, reactions are safe and controllable, the structure of the product is stable and it does not destroy amino acids, functional activity is high, and the hydrolysates are conducive to human absorption | 酶活性较弱,反应速度慢,水解不彻底,价格昂贵、生产成本高 The activity of enzyme is weak, reaction speed is slow, the hydrolysis is not complete, the price of enzyme is expensive, and has high production cost | [ |
化学法 Chemical method | 操作简便、成本低 Easy control, and low cost | 引入有毒物质,安全性低,部分氨基酸被破坏,水解产物成分复杂,应用受限 It introduces toxic substances, safety is low, partial amino acids are destroyed, the hydrolysate composition is complex, and the application scope is limited | [ |
发酵法 Fermentation | 增加产物风味、去腥,操作简便,可批量生产,成本低 Increase product flavor, reduce fishy, easy to operate, and can be mass production at low cost | 产率低,纯化困难,部分产酶菌有毒 Low yield, difficult to be purified, and some bacteria of producing enzyme is toxic | [ |
Table 1 Comparison of different extraction methods of bioactive peptides from fish skin
常规提取方 Conventional extraction method | 优点 Advantages | 缺点 Disadvantages | 文献来源 Reference |
---|---|---|---|
酶法 Enzymatic method | 水解条件温和、反应安全可控,产物结构稳定,不破坏氨基酸,功能活性高,利于人体吸收 The hydrolysis conditions are mild, reactions are safe and controllable, the structure of the product is stable and it does not destroy amino acids, functional activity is high, and the hydrolysates are conducive to human absorption | 酶活性较弱,反应速度慢,水解不彻底,价格昂贵、生产成本高 The activity of enzyme is weak, reaction speed is slow, the hydrolysis is not complete, the price of enzyme is expensive, and has high production cost | [ |
化学法 Chemical method | 操作简便、成本低 Easy control, and low cost | 引入有毒物质,安全性低,部分氨基酸被破坏,水解产物成分复杂,应用受限 It introduces toxic substances, safety is low, partial amino acids are destroyed, the hydrolysate composition is complex, and the application scope is limited | [ |
发酵法 Fermentation | 增加产物风味、去腥,操作简便,可批量生产,成本低 Increase product flavor, reduce fishy, easy to operate, and can be mass production at low cost | 产率低,纯化困难,部分产酶菌有毒 Low yield, difficult to be purified, and some bacteria of producing enzyme is toxic | [ |
来源 Species | 提取方法 Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度Degree of hydrolysis, DH/% | 产率 Yield/% | 自由基清除率 Free radical scavenging rate | 氨基酸序列 Amino acid sequences | 文献来源Reference | ||
---|---|---|---|---|---|---|---|---|---|
1,1-二苯基-2-三硝基苯肼自由基1,1-diphenyl-2-picrylhydrazyl, DPPH·(IC50) | 羟自由基 Hydroxyl free radical, ·OH(IC50) | 2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基 ABTS | |||||||
巴沙鱼皮 Pangasius bocourti skin | 碱性蛋白酶水解 Alcalase hydrolysis | 超滤膜过滤、SDS-PAGE Ultrafiltration membrane filtration, SDS-PAGE | 48.06±1.97 | — | — | — | (45.98±0.04)μmol TE/g | — | [ |
黄鳍金枪鱼皮 Yellowfin Tuna(Thunnus albacares)skin | 碱性蛋白酶水解 Alcalase hydrolysis | 超滤膜过滤、SDS-PAGE Ultrafiltration membrane filtration, SDS-PAGE | — | 52.7 | — | — | — | — | [ |
石斑鱼皮 Seabass(Lates calcarifer)skin | 碱性蛋白酶水解 Alcalase hydrolysis | 交联葡聚糖G-25和超高效液相色谱-电喷雾离子质量-质谱/质谱 Sephadex G-25 column, UPLC-ESI-MS/MS | — | 59.7 | — | — | (2.59 ±0.04)mmol TE/μmol | Val-Leu-Gly-Pro-Phe(532.313 0 Da) | [ |
— | — | (81.41±0.34)mmol TE/μmol | Gly-Leu-Phe-Gly-Pro-Arg(646.367 1 Da) | ||||||
— | — | (10.47±0.35)mmol TE/μmol | Gly-Ala-Thr-Gly-Pro-Gln-Gly-Pro-Leu-Gly-Pro-Arg(1 107.590 5 Da) | ||||||
— | — | (0.5±0.02)mmol TE/μmol | Gln-Leu-Gly-Pro-Leu-Gly-Pro-Val(780.461 4 Da) | ||||||
罗非鱼皮 Tilapia(Oreochromis niloticus)skin | 蛋白酶E、中性蛋白酶 Protease E and multifect neutral | 超滤、葡聚糖凝胶柱层析G-25、反向高效液相色谱、Q-TOF MS/ESI Ultrafiltration, Sephadex G-25 gel filtration column, RP-HPLC and Q-TOF MS/ESI | 22.11 ± 0.19 | — | — | 4.61 μg/mL | — | Glu-Gly-Leu(317.33 Da) | [ |
— | 6.45 μg/mL | — | Tyr-Gly-Asp-Glu-Tyr(645.21 Da) | ||||||
马面鱼皮 Navodon Septentrio nalis(Thamnaco nus septentrionallis)skin | 酸性蛋白酶 和Protease A ‘Amano’2G Acid Protease and Protease A ‘Amano’2G | 超滤和凝胶柱层析法,超高效液相色谱-质谱Ultrafiltration, gel filtration chromatography, and UPLC-MS | — | — | 1.80 mg/mL | — | — | Asn-Glu-Gly-Ala-Cys-Gly/ Gly-Glu-Gly-Ala-Cys-Asn | [ |
Table 2 Anti-oxidative activities of bio-active peptides from fish skin
来源 Species | 提取方法 Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度Degree of hydrolysis, DH/% | 产率 Yield/% | 自由基清除率 Free radical scavenging rate | 氨基酸序列 Amino acid sequences | 文献来源Reference | ||
---|---|---|---|---|---|---|---|---|---|
1,1-二苯基-2-三硝基苯肼自由基1,1-diphenyl-2-picrylhydrazyl, DPPH·(IC50) | 羟自由基 Hydroxyl free radical, ·OH(IC50) | 2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基 ABTS | |||||||
巴沙鱼皮 Pangasius bocourti skin | 碱性蛋白酶水解 Alcalase hydrolysis | 超滤膜过滤、SDS-PAGE Ultrafiltration membrane filtration, SDS-PAGE | 48.06±1.97 | — | — | — | (45.98±0.04)μmol TE/g | — | [ |
黄鳍金枪鱼皮 Yellowfin Tuna(Thunnus albacares)skin | 碱性蛋白酶水解 Alcalase hydrolysis | 超滤膜过滤、SDS-PAGE Ultrafiltration membrane filtration, SDS-PAGE | — | 52.7 | — | — | — | — | [ |
石斑鱼皮 Seabass(Lates calcarifer)skin | 碱性蛋白酶水解 Alcalase hydrolysis | 交联葡聚糖G-25和超高效液相色谱-电喷雾离子质量-质谱/质谱 Sephadex G-25 column, UPLC-ESI-MS/MS | — | 59.7 | — | — | (2.59 ±0.04)mmol TE/μmol | Val-Leu-Gly-Pro-Phe(532.313 0 Da) | [ |
— | — | (81.41±0.34)mmol TE/μmol | Gly-Leu-Phe-Gly-Pro-Arg(646.367 1 Da) | ||||||
— | — | (10.47±0.35)mmol TE/μmol | Gly-Ala-Thr-Gly-Pro-Gln-Gly-Pro-Leu-Gly-Pro-Arg(1 107.590 5 Da) | ||||||
— | — | (0.5±0.02)mmol TE/μmol | Gln-Leu-Gly-Pro-Leu-Gly-Pro-Val(780.461 4 Da) | ||||||
罗非鱼皮 Tilapia(Oreochromis niloticus)skin | 蛋白酶E、中性蛋白酶 Protease E and multifect neutral | 超滤、葡聚糖凝胶柱层析G-25、反向高效液相色谱、Q-TOF MS/ESI Ultrafiltration, Sephadex G-25 gel filtration column, RP-HPLC and Q-TOF MS/ESI | 22.11 ± 0.19 | — | — | 4.61 μg/mL | — | Glu-Gly-Leu(317.33 Da) | [ |
— | 6.45 μg/mL | — | Tyr-Gly-Asp-Glu-Tyr(645.21 Da) | ||||||
马面鱼皮 Navodon Septentrio nalis(Thamnaco nus septentrionallis)skin | 酸性蛋白酶 和Protease A ‘Amano’2G Acid Protease and Protease A ‘Amano’2G | 超滤和凝胶柱层析法,超高效液相色谱-质谱Ultrafiltration, gel filtration chromatography, and UPLC-MS | — | — | 1.80 mg/mL | — | — | Asn-Glu-Gly-Ala-Cys-Gly/ Gly-Glu-Gly-Ala-Cys-Asn | [ |
来源 Species | 提取方法 Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度 Degree of hydrolysis,DH/% | 产率 Yield/% | 半抑制浓度IC50/(μmol·L-1) | 氨基酸序列Amino acid sequences | 文献来源Reference |
---|---|---|---|---|---|---|---|
太平洋鳕鱼皮 Pacific cord fish(Gadus macrocephalus)skin | 酶法 Enzyme method | 超滤、阴离子交换层析、反向高效液相色谱、质谱Ultrafiltration, anion exchange chromatography, RP-HPLC and MS | — | — | 6.9 | GASSGMPG(662 Da) | [ |
14.5 | LAYA(436 Da) | ||||||
阿拉斯加鳕鱼皮 Alaska pollack skins | 酶法 Enzyme method | Sephadex G-25凝胶柱层析、反向高效液相色谱和高效液相色谱-四极杆飞行时间质谱 Sephadex G-25 gel filtration chromatography, RP-HPLC and HPLC-Q-TOF-MS | 108.5 | GPLGVP(538.31 Da) | [ | ||
鳐鱼皮 Skate (Rajiformes)skin | 酶法Enzyme method | Sephadex G-25凝胶柱层析、反向高效液相色谱、Q-TOF MS/ESI Sephadex G-25 gel filtration chromatography, RP-HPLC and Q-TOF MS/ESI | — | — | 95 | PGPLGLTGP(975.38 Da) | [ |
148 | QLGFLGPR(874.45 Da) | ||||||
鳐科鱼皮 Okamejei kenojei skin | 碱性蛋白酶水解 Alcalase hydrolysis | 超滤膜过滤、SuperdexTM Peptide 10/300 GL凝胶柱层析 Ultrafiltration, SuperdexTM Peptide 10/300 GL gel filtration chromatography | — | — | 4.22 | LGPLGHQ(720 Da) | [ |
3.09 | MVGSAPGVL(829 Da) |
Table 3 ACE inhibitory activities of bioactive peptides from fish skin
来源 Species | 提取方法 Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度 Degree of hydrolysis,DH/% | 产率 Yield/% | 半抑制浓度IC50/(μmol·L-1) | 氨基酸序列Amino acid sequences | 文献来源Reference |
---|---|---|---|---|---|---|---|
太平洋鳕鱼皮 Pacific cord fish(Gadus macrocephalus)skin | 酶法 Enzyme method | 超滤、阴离子交换层析、反向高效液相色谱、质谱Ultrafiltration, anion exchange chromatography, RP-HPLC and MS | — | — | 6.9 | GASSGMPG(662 Da) | [ |
14.5 | LAYA(436 Da) | ||||||
阿拉斯加鳕鱼皮 Alaska pollack skins | 酶法 Enzyme method | Sephadex G-25凝胶柱层析、反向高效液相色谱和高效液相色谱-四极杆飞行时间质谱 Sephadex G-25 gel filtration chromatography, RP-HPLC and HPLC-Q-TOF-MS | 108.5 | GPLGVP(538.31 Da) | [ | ||
鳐鱼皮 Skate (Rajiformes)skin | 酶法Enzyme method | Sephadex G-25凝胶柱层析、反向高效液相色谱、Q-TOF MS/ESI Sephadex G-25 gel filtration chromatography, RP-HPLC and Q-TOF MS/ESI | — | — | 95 | PGPLGLTGP(975.38 Da) | [ |
148 | QLGFLGPR(874.45 Da) | ||||||
鳐科鱼皮 Okamejei kenojei skin | 碱性蛋白酶水解 Alcalase hydrolysis | 超滤膜过滤、SuperdexTM Peptide 10/300 GL凝胶柱层析 Ultrafiltration, SuperdexTM Peptide 10/300 GL gel filtration chromatography | — | — | 4.22 | LGPLGHQ(720 Da) | [ |
3.09 | MVGSAPGVL(829 Da) |
来源 Species | 提取方法Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度Degree of hydrolysis, DH/% | 产率 Yield/% | MECs/ (μg·mL-1) | MIC/ (μg·mL-1) | 微生物 Microorganism | 氨基酸序列 Amino acid sequences | 文献来源 Reference |
---|---|---|---|---|---|---|---|---|---|
黄鳍金枪鱼 Yellowfin tuna(Thunnus albacares)skin | 酶法 Enzyme method | 反向高效液相色谱、激光去电离飞行时间质谱法 RP-HPLC, MALDI-TOF | — | — | 1.2 | — | 枯草芽孢杆菌 B. subtilis | YFGAP | [ |
— | — | 白念珠菌 C. albicans | |||||||
3 | — | 大肠杆菌 E. coli | |||||||
鲣鱼鱼皮 Skipjack tuna(Katsuwonus pelamis)skin | 酶法 Enzyme method | 反向高效液相色谱、激光去电离飞行时间质谱分 RP-HPLC, MALDI-TOF | — | — | 3 | — | 枯草芽孢杆菌 B. subtilis | SJGAP | [ |
16 | — | 白念珠菌 C. albicans | |||||||
2.7 | — | 大肠杆菌 E. coli | |||||||
冬季比目鱼 Winter flounder(Pleuronectes americanus)skin | 酸法 Acid-extraction method | G-50分子排阻色谱、高效液相色谱 G-50 gel filtration chromatography and HPLC | — | — | — | 1.1-2.2* | 枯草芽孢杆菌 B. subtilis | GWGSFFKKAAH VGKHVGKAALTHY | [ |
— | — | 白念珠菌 C. albicans | |||||||
— | 2.2-3.3* | 大肠杆菌 E. coli |
Table 4 Antimicrobial activities of bioactive peptides from fish skin
来源 Species | 提取方法Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度Degree of hydrolysis, DH/% | 产率 Yield/% | MECs/ (μg·mL-1) | MIC/ (μg·mL-1) | 微生物 Microorganism | 氨基酸序列 Amino acid sequences | 文献来源 Reference |
---|---|---|---|---|---|---|---|---|---|
黄鳍金枪鱼 Yellowfin tuna(Thunnus albacares)skin | 酶法 Enzyme method | 反向高效液相色谱、激光去电离飞行时间质谱法 RP-HPLC, MALDI-TOF | — | — | 1.2 | — | 枯草芽孢杆菌 B. subtilis | YFGAP | [ |
— | — | 白念珠菌 C. albicans | |||||||
3 | — | 大肠杆菌 E. coli | |||||||
鲣鱼鱼皮 Skipjack tuna(Katsuwonus pelamis)skin | 酶法 Enzyme method | 反向高效液相色谱、激光去电离飞行时间质谱分 RP-HPLC, MALDI-TOF | — | — | 3 | — | 枯草芽孢杆菌 B. subtilis | SJGAP | [ |
16 | — | 白念珠菌 C. albicans | |||||||
2.7 | — | 大肠杆菌 E. coli | |||||||
冬季比目鱼 Winter flounder(Pleuronectes americanus)skin | 酸法 Acid-extraction method | G-50分子排阻色谱、高效液相色谱 G-50 gel filtration chromatography and HPLC | — | — | — | 1.1-2.2* | 枯草芽孢杆菌 B. subtilis | GWGSFFKKAAH VGKHVGKAALTHY | [ |
— | — | 白念珠菌 C. albicans | |||||||
— | 2.2-3.3* | 大肠杆菌 E. coli |
功能活性 Functional activity | 来源 Species | 提取方法 Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度Degree of hydrolysis, DH/% | 产率 Yield/% | 氨基酸序列 Amino acid sequences | 文献来源 Reference |
---|---|---|---|---|---|---|---|
神经保护 活性Neuroprotective activity | 鲑鱼皮 Chum Salmon(Oncorhynchus keta)skin | 酶法 Enzyme method | — | — | — | — | [ |
抗高血糖 Antihyperglycemic | 鲟鱼皮等 Sturgeon(Acipenser sinensis)skin, etc | 酶法 Enzyme method | 交联葡聚糖G-25、反向高效液相色谱 Gel filtration chromatography G-25, RP-HPLC | — | 11.9 | Gly-X-Y(X、Y为可选的氨基酸残基) Gly-X-Y(X、Y is an optional amino acid residue) | [ |
免疫调节性 Immunomodulation activity | 石斑鱼皮 Seabass(Lates calcarifer)skin | 酶法 Enzyme method | — | — | — | — | [ |
基质金属蛋白酶抑制活性 Matrix metalloproteinases, MMP-1 inhibitory activity | 鳕鱼皮 Pacific cod(Gadus Macrocephalus)skin | 酶法 Enzyme method | 阴离子交换层析、反向高效液相色谱、高效液相色谱-电喷雾串联质谱法 Anion exchange chromatography, RP-HPLC、HPLC-LC-ESI-MS/MS | — | — | EIGPSGGRGKPGKDGDAGPK, GFSGLDGAKGD | [ |
Table 5 Other activities of peptides from fish skin
功能活性 Functional activity | 来源 Species | 提取方法 Extraction method | 分离、鉴定方法 Separation and identification methods | 水解度Degree of hydrolysis, DH/% | 产率 Yield/% | 氨基酸序列 Amino acid sequences | 文献来源 Reference |
---|---|---|---|---|---|---|---|
神经保护 活性Neuroprotective activity | 鲑鱼皮 Chum Salmon(Oncorhynchus keta)skin | 酶法 Enzyme method | — | — | — | — | [ |
抗高血糖 Antihyperglycemic | 鲟鱼皮等 Sturgeon(Acipenser sinensis)skin, etc | 酶法 Enzyme method | 交联葡聚糖G-25、反向高效液相色谱 Gel filtration chromatography G-25, RP-HPLC | — | 11.9 | Gly-X-Y(X、Y为可选的氨基酸残基) Gly-X-Y(X、Y is an optional amino acid residue) | [ |
免疫调节性 Immunomodulation activity | 石斑鱼皮 Seabass(Lates calcarifer)skin | 酶法 Enzyme method | — | — | — | — | [ |
基质金属蛋白酶抑制活性 Matrix metalloproteinases, MMP-1 inhibitory activity | 鳕鱼皮 Pacific cod(Gadus Macrocephalus)skin | 酶法 Enzyme method | 阴离子交换层析、反向高效液相色谱、高效液相色谱-电喷雾串联质谱法 Anion exchange chromatography, RP-HPLC、HPLC-LC-ESI-MS/MS | — | — | EIGPSGGRGKPGKDGDAGPK, GFSGLDGAKGD | [ |
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