Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (8): 148-158.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1534
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CHEN Xiao-ling1(), LIAO Dong-qing2, HUANG Shang-fei1, CHEN Ying1, LU Zhi-long1, CHEN Dong1
Received:
2022-12-18
Online:
2023-08-26
Published:
2023-09-05
Contact:
CHEN Xiao-ling
E-mail:xiaolingchen1116@163.com
CHEN Xiao-ling, LIAO Dong-qing, HUANG Shang-fei, CHEN Ying, LU Zhi-long, CHEN Dong. Advances in CRISPR/Cas9 System Modifying Saccharomycescerevisiae[J]. Biotechnology Bulletin, 2023, 39(8): 148-158.
Site-specific DNA cleavage by CRISPR/Cas9 system Case N indicates base A, C, G,T(DNA)or U(RNA); indicate Cas9 whose cleavage sites are marked with scissors(N in red show the probely cut sits). Regions of crRNA complementarity to tracrRNA(blue), protospacer DNA(green)and PAM sequence(in yellow background)are represented
名称 Name | 链接 Link | 物种Species | 优点 Advantage | 缺点 Disadvantage | 参考文献Reference |
---|---|---|---|---|---|
GuideScan | - | 任何 微生物 | 能以目标酿酒酵母的基因组为参考基因组、有助于减少脱靶位点,设计的靶序列比the mit.edu web interface[ | 需在本地计算机安装,需使用命令行,需提供参考基因组 | [ |
Yeastriction | http://yeastriction.tnw.tudelft.nl/ | 酿酒酵母 | 设计的靶序列比ChopChop[ | 容易脱靶(除非参考基因组是目标酿酒酵母的基因组) | [ |
CRISPy | http://staff.biosustain.dtu.dk/laeb/crispy_yeast/ | 酿酒酵母 | 不需上传参考基因组 | 只能以酿酒酵母S288C的基因组为参考基因组,为其他酿酒酵母设计靶序列时,容易脱靶 | [ |
CRISPy-web | http://crispy.secondarymetabolites.org/ | 任何 微生物 | 免安装,不需使用命令行,能以目标酿酒酵母的基因组为参考基因组、有助于减少脱靶位点 | 需上传参考基因组 | [ |
Table 1 SgRNA design tools available for S. cerevisiae
名称 Name | 链接 Link | 物种Species | 优点 Advantage | 缺点 Disadvantage | 参考文献Reference |
---|---|---|---|---|---|
GuideScan | - | 任何 微生物 | 能以目标酿酒酵母的基因组为参考基因组、有助于减少脱靶位点,设计的靶序列比the mit.edu web interface[ | 需在本地计算机安装,需使用命令行,需提供参考基因组 | [ |
Yeastriction | http://yeastriction.tnw.tudelft.nl/ | 酿酒酵母 | 设计的靶序列比ChopChop[ | 容易脱靶(除非参考基因组是目标酿酒酵母的基因组) | [ |
CRISPy | http://staff.biosustain.dtu.dk/laeb/crispy_yeast/ | 酿酒酵母 | 不需上传参考基因组 | 只能以酿酒酵母S288C的基因组为参考基因组,为其他酿酒酵母设计靶序列时,容易脱靶 | [ |
CRISPy-web | http://crispy.secondarymetabolites.org/ | 任何 微生物 | 免安装,不需使用命令行,能以目标酿酒酵母的基因组为参考基因组、有助于减少脱靶位点 | 需上传参考基因组 | [ |
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