Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (8): 148-158.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1534

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Advances in CRISPR/Cas9 System Modifying Saccharomycescerevisiae

CHEN Xiao-ling1(), LIAO Dong-qing2, HUANG Shang-fei1, CHEN Ying1, LU Zhi-long1, CHEN Dong1   

  1. 1. Guangxi Academy of Sciences,National key Laboratory of Non-food Biomass Energy Technology, State Key Laboratory of Non-food Biomass and Enzyme Technology, Nanning 530007
    2. Guangxi Light Industry Science and Technology Research Institute Co., Ltd., Nanning 530031
  • Received:2022-12-18 Online:2023-08-26 Published:2023-09-05
  • Contact: CHEN Xiao-ling E-mail:xiaolingchen1116@163.com

Abstract:

Saccharomyces cerevisiae is one of widely used industrial strains, it is extremely important to increase its performance via modifying S. cerevisiae; however, there are cumbersome steps and long-cycle by traditional modifying method. CRISPR/Cas9 system is an adaptive defense system found in bacteria and archaea. At present, the CRISPR/Cas9 system has become a powerful tool for genome editing, which can simultaneously modify multiple genes of S. cerevisiae. This review provided a brief overview of the function and construction of CRISPR/Cas9 system in S. cerevisiae gene editing, introduced the design rules and representative design tools of sgRNA targeting sequences, as well as summarized the multiple-gene editing strategy. Moreover, the review discussed the challenges in application of CRISPR/Cas9 system, including off target and low editing efficiency, and their control measures, which may provide the references for better applying CRISPR/Cas9 system modifying S. cerevisiae.

Key words: construction of CRISPR/Cas9 system, multiplex gene editing, design of target sequence, off-target