Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (2): 212-222.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0896
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ZHAO Yao(), WEN Lang, LUO Shao-dan, LI Zi-xing, LIU Chao-chao()
Received:
2023-09-18
Online:
2024-02-26
Published:
2024-03-13
Contact:
LIU Chao-chao
E-mail:211211801121@stu.just.edu.cn;ccliu@just.edu.cn
ZHAO Yao, WEN Lang, LUO Shao-dan, LI Zi-xing, LIU Chao-chao. Identification of HMA Gene Family and Cadmium Transport Function of SlHMA1 in Tomato[J]. Biotechnology Bulletin, 2024, 40(2): 212-222.
基因Gene | 正向引物Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
SlHMA1 | CAGGAGGCCTTATAATGGTAGG | TAGTACATCAGCAACAGCTACC |
SlHMA3 | GTGTTCTGACTCAACAGACAAC | TTTCCACATGTCATTGAGCTTG |
SlHMA4 | GATGGCGTCGTTATTAATGGTC | ACAGTTCCACCAATGACCTTAT |
SlHMA5.1 | ATTCATCATTGGACGACGTTTC | AGCTGGTTCTTAACACCGAATA |
SlHMA5.2 | GCTTATGGATTTAGCCCCAAAG | TCGTTCTTTTGTATCAATCGGC |
SlHMA6 | TAGTCAGTGGAGATTTAGGGGA | CAATGATCTGATCTCCGACTGA |
SlHMA7 | CTGCAGAATACGTGTTGATGAG | GGGATGGAAATCACATTGTACG |
SlHMA8 | ATTCTCTCTCTCACACGATCAC | CAATAGCTGACTAGTACGACGT |
SlActin | CCATTCTCCGTCTTGACTTGG | TCTTTCCTAATATCCACGTCAC |
Table 1 Primers used for RT-qPCR
基因Gene | 正向引物Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
SlHMA1 | CAGGAGGCCTTATAATGGTAGG | TAGTACATCAGCAACAGCTACC |
SlHMA3 | GTGTTCTGACTCAACAGACAAC | TTTCCACATGTCATTGAGCTTG |
SlHMA4 | GATGGCGTCGTTATTAATGGTC | ACAGTTCCACCAATGACCTTAT |
SlHMA5.1 | ATTCATCATTGGACGACGTTTC | AGCTGGTTCTTAACACCGAATA |
SlHMA5.2 | GCTTATGGATTTAGCCCCAAAG | TCGTTCTTTTGTATCAATCGGC |
SlHMA6 | TAGTCAGTGGAGATTTAGGGGA | CAATGATCTGATCTCCGACTGA |
SlHMA7 | CTGCAGAATACGTGTTGATGAG | GGGATGGAAATCACATTGTACG |
SlHMA8 | ATTCTCTCTCTCACACGATCAC | CAATAGCTGACTAGTACGACGT |
SlActin | CCATTCTCCGTCTTGACTTGG | TCTTTCCTAATATCCACGTCAC |
基因ID Gene ID | 基因名称 Gene name | 氨基酸数目 No. of amino acid | 分子量 Molecular weight/kD | 等电点 pI | 稳定性 Instability index | 脂肪系数 Aliphatic index | 亲水性 GRAVY | 亚细胞定位 Subcellular localization | 跨膜结构域 TM |
---|---|---|---|---|---|---|---|---|---|
Solyc02g092920.2 | SlHMA1 | 821 | 89.08 | 8.44 | 34.91 | 100.4 | 0.13 | 叶绿体 Chloroplast | 0 |
Solyc07g009130.2 | SlHMA3 | 1 302 | 140.44 | 6.94 | 45.63 | 80.46 | -0.231 | 细胞质膜 Plasma membrane | 8 |
Solyc11g062100.1 | SlHMA4 | 698 | 76.04 | 5.68 | 36.13 | 104.44 | 0.227 | 细胞质膜 Plasma membrane | 7 |
Solyc08g080870.2 | SlHMA5.1 | 954 | 103.58 | 5.58 | 39.69 | 102.69 | 0.139 | 细胞质膜 Plasma membrane | 6 |
Solyc08g080890.2 | SlHMA5.2 | 890 | 96.58 | 5.80 | 32.38 | 103.96 | 0.200 | 细胞质膜 Plasma membrane | 8 |
Solyc01g105160.4 | SlHMA6 | 964 | 101.94 | 8.30 | 33.79 | 97.68 | 0.092 | 叶绿体 Chloroplast | 0 |
Solyc02g068490.3 | SlHMA7 | 1 015 | 108.59 | 5.16 | 30.72 | 106.97 | 0.324 | 细胞质膜 Plasma membrane | 10 |
Solyc08g061610.2 | SlHMA8 | 894 | 95.20 | 5.94 | 38.01 | 103.66 | 0.118 | 叶绿体 Chloroplast | 0 |
Table 2 Physicochemical properties of SlHMAs protein
基因ID Gene ID | 基因名称 Gene name | 氨基酸数目 No. of amino acid | 分子量 Molecular weight/kD | 等电点 pI | 稳定性 Instability index | 脂肪系数 Aliphatic index | 亲水性 GRAVY | 亚细胞定位 Subcellular localization | 跨膜结构域 TM |
---|---|---|---|---|---|---|---|---|---|
Solyc02g092920.2 | SlHMA1 | 821 | 89.08 | 8.44 | 34.91 | 100.4 | 0.13 | 叶绿体 Chloroplast | 0 |
Solyc07g009130.2 | SlHMA3 | 1 302 | 140.44 | 6.94 | 45.63 | 80.46 | -0.231 | 细胞质膜 Plasma membrane | 8 |
Solyc11g062100.1 | SlHMA4 | 698 | 76.04 | 5.68 | 36.13 | 104.44 | 0.227 | 细胞质膜 Plasma membrane | 7 |
Solyc08g080870.2 | SlHMA5.1 | 954 | 103.58 | 5.58 | 39.69 | 102.69 | 0.139 | 细胞质膜 Plasma membrane | 6 |
Solyc08g080890.2 | SlHMA5.2 | 890 | 96.58 | 5.80 | 32.38 | 103.96 | 0.200 | 细胞质膜 Plasma membrane | 8 |
Solyc01g105160.4 | SlHMA6 | 964 | 101.94 | 8.30 | 33.79 | 97.68 | 0.092 | 叶绿体 Chloroplast | 0 |
Solyc02g068490.3 | SlHMA7 | 1 015 | 108.59 | 5.16 | 30.72 | 106.97 | 0.324 | 细胞质膜 Plasma membrane | 10 |
Solyc08g061610.2 | SlHMA8 | 894 | 95.20 | 5.94 | 38.01 | 103.66 | 0.118 | 叶绿体 Chloroplast | 0 |
Fig. 6 Gene expression pattern of SlHMAs in the root and leaf of tomato under cadmium stress The different letters indicate significant differences(P<0.05). The same below
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