Mechanism of CMV Intervention in Peach Aphid Population Growth by Influencing the Expression of Effector MpC002

doi:10.13560/j.cnki.biotech.bull.1985.2023-1057

Abstract

Abstract:

【Objective】Peach aphid（Myzus persicae）is an important vector of cucumber mosaic virus（CMV）, and the effector MpC002 plays a key role in the feeding process. CMV and MpC002 are both present in aphid saliva, but little is known about their interaction and how MpC002 facilitate virus transmission. Therefore, this study aims to preliminarily investigate the mechanism by which CMV influences the expression of MpC002 for interfering the population growth of GPA. 【Method】The CMV copy number was calculated by the standard curve y=-3.163 1x+39.763, via applying the absolute quantitative polymerase chain reaction（qPCR）. 【Result】The CMV content in the peach aphid fed with CMV-infected pepper reached the highest at 10 s, then the CMV content decreased gradually along with the feeding time, and its virulence acquisition process conformed to the characteristics of non-persistent transmission. In addition, the expressions of MpC002 in the peach aphids fed on CMV-infested peppers within 5 min-24 h significantly decreased to 37%-58%, compared with those before feeding. While feeding on CMV-infested peppers, the average developmental duration of third, fourth instar and the entire nymph stages were 1.58, 2.07 and 6.47 d, which were significantly longer than those fed on the untreated peppers（1.25 d, 1.47 d and 5.33 d）; in contrast, the developmental duration of the first and second instars did not show significantly difference between CMV-infested and untreated peppers. The average laying period, the total peach aphid number and the average peach aphid in a single day were 10.03 d, 16.87 and 1.67, which were significantly shorter than those of untreated peppers（14.27 d, 39.73 and 2.82）. In addition, analysis of GPA life table parameters revealed that the net growth rate, intrinsic rate of increase, and finite rate of increase were 16.87, 0.21, and 1.21, respectively, which were significantly lower than those of peach aphids feeding on untreated peppers（39.73, 0.31, and 1.36, respectively）, whereas the mean generation time（13.02 d）and the population doubling time（3.26 d）were both significantly longer than those of peach aphids feeding untreated peppers（12.00 d and 2.30 d）. 【Conclusion】CMV significantly inhibited the expressions of MpC002, and then prolongated the developmental duration and decreased the reproduction, which in turn adversely affected its population growth.

Key words: CMV, pepper, Myzus persicae, effector MpC002, population growth

MAO Li-jie, LIANG Xiao, LIU Ying, WU Chun-ling, HAN Xiao-yan, CHEN Qing. Mechanism of CMV Intervention in Peach Aphid Population Growth by Influencing the Expression of Effector MpC002[J]. Biotechnology Bulletin, 2024, 40(4): 271-277.

Figures/Tables 5

Fig. 1 CMV content in M. persicae after fed on CMV-infested pepper at different time A: Standard curve of CMV quantitative detection; B: the CMV copies in M. persicae at different times, data indicate mean value ± SE; *, ** and ns indicate significant（P<0.05）, extremely significant difference（P<0.01）and no significant difference differences in CMV copy number within the same feeding time point, respectively. The same below

Fig. 2 Relative expressions of the effector MpC002 in M. persicae after feeding on CMV-infested and untreated pepper A: The transcription of MpC002 after feeding on CMV- infested pepper. B: The transcription of MpC002 after feeding on untreated pepper

Fig. 3 Developmental duration of M. persicae after feeding on CMV-infested or untreated peppers

Fig. 4 Influence on M. persicae reproduction and lifespan after feeding on CMV-infested and untreated peppers A: Average production period per female; B: average total production per female; C: average daily production; D: average lifespan of adult aphid

Table 1 Life table parameters of aphids after feeding on CMV-infested and untreated peppers

处理 Treatment	净增殖率R₀ Net reproduction rate（R₀）	平均世代周期T Mean generation time（T）/d	内禀增长率r_m Intrinsic rate of increase（r_m）/（d^-1）	周限增长率λ Finite rate of increase（λ）/（d^-1）	种群加倍时间Dt Population doubling time（Dt）/d
未处理辣椒 Untreated peppers	39.73±2.08^**	12.00±0.24	0.31±0.01^**	1.36±0.01^**	2.30±0.06
CMV感染辣椒 CMV-infested peppers	16.87±0.98	13.02±0.40^*	0.21±0.00	1.24±0.01	3.26±0.07^**

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