Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (5): 74-83.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1148
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LI Si-qi(), ZHANG Wen-chen, YANG Liu, FU Qing-xin, HONG Xin, ZHANG Hai-wang()
Received:
2023-12-05
Online:
2024-05-26
Published:
2024-06-13
Contact:
ZHANG Hai-wang
E-mail:675739325@qq.com;zhanghaiwang204@126.com
LI Si-qi, ZHANG Wen-chen, YANG Liu, FU Qing-xin, HONG Xin, ZHANG Hai-wang. Genetic Diversity Analysis and DNA Fingerprint Construction Based on SSR Markers for Xanthoceras sorbifolia[J]. Biotechnology Bulletin, 2024, 40(5): 74-83.
编号 Code | 材料名称 Accession | 来源地 Origin | 形态特征 Main characteristics |
---|---|---|---|
1 | 优系-1红 | 朝阳县 | 红花,三棱果型 |
2 | 优系-1黄 | 朝阳县 | 黄花,三棱果型 |
3 | 优系-1白 | 朝阳县 | 白花,平顶长方果型 |
4 | 优系-2红 | 朝阳县 | 红花,桃形果型 |
5 | 圆大硕种 | 彰武县 | 白花,奇数羽状复叶 |
6 | 悄然薄壳 | 彰武县 | 白花,果壳较薄 |
7 | 中石1号 | 彰武县 | 白花,平顶正方形果型 |
8 | 中石4号 | 彰武县 | 白花,小球果型 |
9 | 中石7号 | 彰武县 | 白花,三棱果型 |
10 | 中石9号 | 彰武县 | 白花,三棱果型 |
11 | 优系-2白 | 建平县 | 白花,桃形果型 |
12 | 优系-3白 | 建平县 | 白花,小球果型 |
13 | 优系-4白 | 建平县 | 白花,小球果型 |
14 | 优系-3红 | 朝阳县 | 红花,三棱果型 |
15 | 优系-2黄 | 朝阳县 | 黄花,平顶正方形果型 |
16 | 优系-4红 | 建平县 | 红花,三棱果型 |
17 | 优系-5白 | 义县 | 白花,三棱果型 |
18 | 辽红1号 | 建平县 | 红花,长尖果型 |
19 | 辽富1号 | 建平县 | 白花,扁球果型 |
20 | 优系-6白 | 建平县 | 白花,果大,丰产 |
21 | 优系-7白 | 建平县 | 白花,果大,丰产 |
22 | 优系-8白 | 建平县 | 白花,果大,丰产 |
23 | 优系-9白 | 建平县 | 白花,果大,丰产 |
24 | 优系-10白 | 建平县 | 白花,果大,丰产 |
25 | 优系-11白 | 建平县 | 白花,果大,丰产 |
26 | 优系-12白 | 建平县 | 白花,果大,丰产 |
27 | 优系-13白 | 建平县 | 白花,果大,丰产 |
28 | 优系-14白 | 建平县 | 白花,果大,丰产 |
29 | 辽冠1号 | 彰武县 | 白花,果实青色 |
Table 1 X. sorbifolium varieties(lines)
编号 Code | 材料名称 Accession | 来源地 Origin | 形态特征 Main characteristics |
---|---|---|---|
1 | 优系-1红 | 朝阳县 | 红花,三棱果型 |
2 | 优系-1黄 | 朝阳县 | 黄花,三棱果型 |
3 | 优系-1白 | 朝阳县 | 白花,平顶长方果型 |
4 | 优系-2红 | 朝阳县 | 红花,桃形果型 |
5 | 圆大硕种 | 彰武县 | 白花,奇数羽状复叶 |
6 | 悄然薄壳 | 彰武县 | 白花,果壳较薄 |
7 | 中石1号 | 彰武县 | 白花,平顶正方形果型 |
8 | 中石4号 | 彰武县 | 白花,小球果型 |
9 | 中石7号 | 彰武县 | 白花,三棱果型 |
10 | 中石9号 | 彰武县 | 白花,三棱果型 |
11 | 优系-2白 | 建平县 | 白花,桃形果型 |
12 | 优系-3白 | 建平县 | 白花,小球果型 |
13 | 优系-4白 | 建平县 | 白花,小球果型 |
14 | 优系-3红 | 朝阳县 | 红花,三棱果型 |
15 | 优系-2黄 | 朝阳县 | 黄花,平顶正方形果型 |
16 | 优系-4红 | 建平县 | 红花,三棱果型 |
17 | 优系-5白 | 义县 | 白花,三棱果型 |
18 | 辽红1号 | 建平县 | 红花,长尖果型 |
19 | 辽富1号 | 建平县 | 白花,扁球果型 |
20 | 优系-6白 | 建平县 | 白花,果大,丰产 |
21 | 优系-7白 | 建平县 | 白花,果大,丰产 |
22 | 优系-8白 | 建平县 | 白花,果大,丰产 |
23 | 优系-9白 | 建平县 | 白花,果大,丰产 |
24 | 优系-10白 | 建平县 | 白花,果大,丰产 |
25 | 优系-11白 | 建平县 | 白花,果大,丰产 |
26 | 优系-12白 | 建平县 | 白花,果大,丰产 |
27 | 优系-13白 | 建平县 | 白花,果大,丰产 |
28 | 优系-14白 | 建平县 | 白花,果大,丰产 |
29 | 辽冠1号 | 彰武县 | 白花,果实青色 |
序号No. | 引物名称Primer name | 5'标记荧光5' fluorescent marker |
---|---|---|
1 | QXH002 | 5' FAM |
2 | QXH049 | 5' HEX |
3 | QXH177 | 5' HEX |
4 | QXH262 | 5' TAMRA |
5 | QXH274 | 5' FAM |
6 | QXH282 | 5' TAMRA |
7 | QXH365 | 5' FAM |
8 | QXHS371 | 5' TAMRA |
9 | QXH643 | 5' ROX |
10 | QBLB58 | 5' HEX |
11 | QBRS192 | 5' ROX |
Table 2 Information about 11 pairs of SSR primers
序号No. | 引物名称Primer name | 5'标记荧光5' fluorescent marker |
---|---|---|
1 | QXH002 | 5' FAM |
2 | QXH049 | 5' HEX |
3 | QXH177 | 5' HEX |
4 | QXH262 | 5' TAMRA |
5 | QXH274 | 5' FAM |
6 | QXH282 | 5' TAMRA |
7 | QXH365 | 5' FAM |
8 | QXHS371 | 5' TAMRA |
9 | QXH643 | 5' ROX |
10 | QBLB58 | 5' HEX |
11 | QBRS192 | 5' ROX |
位点 Locus | 观测等位基因数 Number of alleles (Na) | 有效等位基因数 Effective number of alleles (Ne) | Shannon 信息指数 Shannon information index (I) | 观测杂合 Observed heterozygosity (Ho) | 期望杂合 Expected heterozygosity (He) | 固定指数 Fixation index (F) | 多态信息含量 Polymorphic information content (PIC) |
---|---|---|---|---|---|---|---|
QXH002 | 3 | 2.591 | 0.950 | 0.942 | 0.602 | -0.599 | 0.659 |
QXH049 | 5 | 3.521 | 1.297 | 0.884 | 0.706 | -0.271 | 0.753 |
QXH177 | 7 | 3.309 | 1.338 | 0.863 | 0.697 | -0.237 | 0.705 |
QXH262 | 8 | 4.579 | 1.648 | 0.838 | 0.780 | -0.080 | 0.811 |
QXH274 | 10 | 4.845 | 1.723 | 0.958 | 0.790 | -0.218 | 0.838 |
QXH282 | 6 | 3.616 | 1.333 | 0.884 | 0.711 | -0.263 | 0.765 |
QXH365 | 4 | 2.322 | 0.959 | 0.340 | 0.560 | 0.396 | 0.601 |
QXHS371 | 5 | 1.933 | 0.759 | 0.440 | 0.444 | -0.018 | 0.545 |
QXH643 | 8 | 4.614 | 1.623 | 0.905 | 0.778 | -0.165 | 0.841 |
QBLB58 | 4 | 1.812 | 0.751 | 0.340 | 0.410 | 0.109 | 0.276 |
QBRS192 | 6 | 1.272 | 0.349 | 0.137 | 0.187 | 0.179 | 0.507 |
Table 3 Genetic diversity information of 11 SSR primers
位点 Locus | 观测等位基因数 Number of alleles (Na) | 有效等位基因数 Effective number of alleles (Ne) | Shannon 信息指数 Shannon information index (I) | 观测杂合 Observed heterozygosity (Ho) | 期望杂合 Expected heterozygosity (He) | 固定指数 Fixation index (F) | 多态信息含量 Polymorphic information content (PIC) |
---|---|---|---|---|---|---|---|
QXH002 | 3 | 2.591 | 0.950 | 0.942 | 0.602 | -0.599 | 0.659 |
QXH049 | 5 | 3.521 | 1.297 | 0.884 | 0.706 | -0.271 | 0.753 |
QXH177 | 7 | 3.309 | 1.338 | 0.863 | 0.697 | -0.237 | 0.705 |
QXH262 | 8 | 4.579 | 1.648 | 0.838 | 0.780 | -0.080 | 0.811 |
QXH274 | 10 | 4.845 | 1.723 | 0.958 | 0.790 | -0.218 | 0.838 |
QXH282 | 6 | 3.616 | 1.333 | 0.884 | 0.711 | -0.263 | 0.765 |
QXH365 | 4 | 2.322 | 0.959 | 0.340 | 0.560 | 0.396 | 0.601 |
QXHS371 | 5 | 1.933 | 0.759 | 0.440 | 0.444 | -0.018 | 0.545 |
QXH643 | 8 | 4.614 | 1.623 | 0.905 | 0.778 | -0.165 | 0.841 |
QBLB58 | 4 | 1.812 | 0.751 | 0.340 | 0.410 | 0.109 | 0.276 |
QBRS192 | 6 | 1.272 | 0.349 | 0.137 | 0.187 | 0.179 | 0.507 |
Fig. 1 Heat map of genetic similarity coefficient of 29 X. sorbifolium samples The horizontal and vertical serial numbers indicate 29 varieties (lines) of X. sorbifolium in the order shown in Table 1. The same below
Fig. 3 Standardized SSR fingerprint for 29 X. sorbifolium samples The horizontal serial numbers in the figure indicate 29 varieties(lines)of X. sorbifolium in the order shown in Table 1, the vertical coordinates indicates the molecular weight of amplification bands of 11 primer pairs, and the black bands indicate the detection of alleles at this locus
编号 Code | 品种 Variety | SSR指纹数据 SSR fingerprint data | 分子身份证 Molecular ID |
---|---|---|---|
1 | 优系-1红 | 110-01100-0001000-01000100-0000100100-001100-1000-10010-101000000-0011-000010 | 1/22/342/65/83/411/41/33/45 |
2 | 优系-1黄 | 101-01100-0000101-00001001-0000011000-011000-1001-10000-010100000-0001-100000 | 1/32/35/75/86/72/31/412/441 |
3 | 优系-1白 | 010-01010-0001100-00100010-0001000010-001010-1100-10100-100100000-0101-100001 | 22/44/53/74/93/51/21/31/42/41/6 |
4 | 优系-2红 | 011-10100-1001000-00010000-0000011000-100100-0100-10000-001001000-0001-100000 | 2/31/31/446/71/4213/641 |
5 | 圆大硕种 | 011-00101-1001000-10000000-1000000010-000101-1100-00100-110000000-0001-110000 | 2/33/51/411/94/61/231/241/2 |
6 | 悄然薄壳 | 110-01000-0001000-10010000-0000010000-001000-1010-10000-001010000-0001-110000 | 1/2241/4631/313/541/2 |
7 | 中石1号 | 011-11000-0001100-10010000-1000010000-101000-0110-00110-010000000-0001-100000 | 2/31/24/51/41/61/32/33/4241 |
8 | 中石4号 | 011-01001-1001000-10000000-1000100000-001010-0100-10010-110000000-0001-001010 | 2/32/51/411/53/521/41/243/5 |
9 | 中石7号 | 101-10100-1001000-10000000-0000110000-100100-1000-10100-101000000-0001-100000 | 1/31/31/415/61/411/31/341 |
10 | 中石9号 | 011-01001-1001000-10000000-1000100000-001001-0100-10010-110000000-0001-001010 | 2/32/51/411/53/621/41/243/5 |
11 | 优系-2白 | 101-10001-1000100-00001100-0000100001-100001-1010-10000-001000010-0100-100000 | 1/31/5155/65/A1/61/313/821 |
12 | 优系-3白 | 011-10010-0101000-00110000-0000110000-100010-1000-10001-001010000-0100-100000 | 2/31/42/43/45/61/511/53/521 |
13 | 优系-4白 | 101-01001-0010010-10001000-0000100000-001001-1000-10010-100100000-0001-100000 | 1/32/53/61/553/611/41/441 |
14 | 优系-3红 | 011-01100-0001100-01010000-0010010000-001100-1010-10100-100000100-1001-100010 | 2/32/34/52/43/63/41/31/31/71/41/5 |
15 | 优系-2黄 | 011-10000-1001000-01000000-0001000001-100000-1000-10000-000101000-0001-101000 | 2/311/424/A1114/641/3 |
16 | 优系-4红 | 110-01100-0001000-01000100-0000100100-001100-1000-10010-001010000-0001-000010 | 1/22/342/65/83/411/43/545 |
17 | 优系-5白 | 101-10001-0001000-11000000-0000100100-100001-0110-10010-000000100-1001-100010 | 1/31/541/25/81/62/31/471/41/5 |
18 | 辽红1号 | 110-01100-0001000-01000100-0000100100-001100-1000-10010-101000000-0001-000010 | 1/22/342/65/83/411/41/345 |
19 | 辽富1号 | 011-10100-0001100-11000000-0000010100-100100-0110-00010-001100000-0001-100010 | 2/31/34/51/26/81/42/343/441/5 |
20 | 优系-6白 | 010-01000-1001000-00010000-0000000101-001000-1000-11000-101000000-0001-110000 | 221/448/A311/21/341/2 |
21 | 优系-7白 | 011-10100-0001100-11000000-0000010100-100100-0110-00010-001100000-0001-100000 | 2/31/34/51/26/81/42/343/441 |
22 | 优系-8白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
23 | 优系-9白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
24 | 优系-10白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
25 | 优系-11白 | 011-10000-0001100-10000010-0001000010-100000-0001-10010-001000000-1001-100000 | 2/314/51/74/9141/431/41 |
26 | 优系-12白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
27 | 优系-13白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
28 | 优系-14白 | 101-10001-1001000-00010100-0000100001-100001-0010-10000-000100001-0001-100000 | 1/31/51/44/65/A1/6314/941 |
29 | 辽冠1号 | 101-10001-0001100-10100000-0100000010-100001-1010-10010-100000100-0001-100100 | 1/31/54/51/32/91/61/31/41/741/4 |
Table 4 Fingerprint database and molecular identities of 29 X. sorbifolium samples at 11 SSR loci
编号 Code | 品种 Variety | SSR指纹数据 SSR fingerprint data | 分子身份证 Molecular ID |
---|---|---|---|
1 | 优系-1红 | 110-01100-0001000-01000100-0000100100-001100-1000-10010-101000000-0011-000010 | 1/22/342/65/83/411/41/33/45 |
2 | 优系-1黄 | 101-01100-0000101-00001001-0000011000-011000-1001-10000-010100000-0001-100000 | 1/32/35/75/86/72/31/412/441 |
3 | 优系-1白 | 010-01010-0001100-00100010-0001000010-001010-1100-10100-100100000-0101-100001 | 22/44/53/74/93/51/21/31/42/41/6 |
4 | 优系-2红 | 011-10100-1001000-00010000-0000011000-100100-0100-10000-001001000-0001-100000 | 2/31/31/446/71/4213/641 |
5 | 圆大硕种 | 011-00101-1001000-10000000-1000000010-000101-1100-00100-110000000-0001-110000 | 2/33/51/411/94/61/231/241/2 |
6 | 悄然薄壳 | 110-01000-0001000-10010000-0000010000-001000-1010-10000-001010000-0001-110000 | 1/2241/4631/313/541/2 |
7 | 中石1号 | 011-11000-0001100-10010000-1000010000-101000-0110-00110-010000000-0001-100000 | 2/31/24/51/41/61/32/33/4241 |
8 | 中石4号 | 011-01001-1001000-10000000-1000100000-001010-0100-10010-110000000-0001-001010 | 2/32/51/411/53/521/41/243/5 |
9 | 中石7号 | 101-10100-1001000-10000000-0000110000-100100-1000-10100-101000000-0001-100000 | 1/31/31/415/61/411/31/341 |
10 | 中石9号 | 011-01001-1001000-10000000-1000100000-001001-0100-10010-110000000-0001-001010 | 2/32/51/411/53/621/41/243/5 |
11 | 优系-2白 | 101-10001-1000100-00001100-0000100001-100001-1010-10000-001000010-0100-100000 | 1/31/5155/65/A1/61/313/821 |
12 | 优系-3白 | 011-10010-0101000-00110000-0000110000-100010-1000-10001-001010000-0100-100000 | 2/31/42/43/45/61/511/53/521 |
13 | 优系-4白 | 101-01001-0010010-10001000-0000100000-001001-1000-10010-100100000-0001-100000 | 1/32/53/61/553/611/41/441 |
14 | 优系-3红 | 011-01100-0001100-01010000-0010010000-001100-1010-10100-100000100-1001-100010 | 2/32/34/52/43/63/41/31/31/71/41/5 |
15 | 优系-2黄 | 011-10000-1001000-01000000-0001000001-100000-1000-10000-000101000-0001-101000 | 2/311/424/A1114/641/3 |
16 | 优系-4红 | 110-01100-0001000-01000100-0000100100-001100-1000-10010-001010000-0001-000010 | 1/22/342/65/83/411/43/545 |
17 | 优系-5白 | 101-10001-0001000-11000000-0000100100-100001-0110-10010-000000100-1001-100010 | 1/31/541/25/81/62/31/471/41/5 |
18 | 辽红1号 | 110-01100-0001000-01000100-0000100100-001100-1000-10010-101000000-0001-000010 | 1/22/342/65/83/411/41/345 |
19 | 辽富1号 | 011-10100-0001100-11000000-0000010100-100100-0110-00010-001100000-0001-100010 | 2/31/34/51/26/81/42/343/441/5 |
20 | 优系-6白 | 010-01000-1001000-00010000-0000000101-001000-1000-11000-101000000-0001-110000 | 221/448/A311/21/341/2 |
21 | 优系-7白 | 011-10100-0001100-11000000-0000010100-100100-0110-00010-001100000-0001-100000 | 2/31/34/51/26/81/42/343/441 |
22 | 优系-8白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
23 | 优系-9白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
24 | 优系-10白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
25 | 优系-11白 | 011-10000-0001100-10000010-0001000010-100000-0001-10010-001000000-1001-100000 | 2/314/51/74/9141/431/41 |
26 | 优系-12白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
27 | 优系-13白 | 101-01001-1000001-00011000-0000101000-001001-1000-10000-000100010-0001-100000 | 1/32/51/74/55/73/6114/841 |
28 | 优系-14白 | 101-10001-1001000-00010100-0000100001-100001-0010-10000-000100001-0001-100000 | 1/31/51/44/65/A1/6314/941 |
29 | 辽冠1号 | 101-10001-0001100-10100000-0100000010-100001-1010-10010-100000100-0001-100100 | 1/31/54/51/32/91/61/31/41/741/4 |
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