Genetic Diversity Analysis and Molecular Identity Card Construction by SSR Markers of 52 Solanum tuberosum L. Varieties（Lines）

doi:10.13560/j.cnki.biotech.bull.1985.2023-0512

Abstract

Abstract:

The evaluation on the genetic diversity of potato germplasm resources（Solanum tuberosum L.）is the prerequisite and basis for genetic breeding, which may provide breeding materials for exploring the genetic background of potato germplasm resources and breeding new varieties. The genetic relationship and diversity of 52 potato varieties（lines）was evaluated by SSR markers, and the fingerprint was constructed. Sixteen primers with abundant polymorphism and good stability were selected from 25 pairs of SSR primers, and 147 polymorphic bands were amplified by 16 pairs of SSR primers from 52 potato varieties（lines）, and the average polymorphic band ratio was 64.71%. The average Nei's genetic diversity（H）and Shannon's index（I）were 0.14 and 0.24, respectively. At the similar genetic coefficient of 0.23, the 52 potato materials were divided into three categories by cluster analysis. Group I included 49 varieties（lines）, 46 varieties（lines）from North China, and 3 imported varieties（lines）from abroad, all of them were suitable for planting in North Shanxi province. Group II included two varieties, ‘Beifang 016’ and ‘Jinshu 16’, characterized by yellow potato skin and high dry matter content. Group III only included ‘Jinshu 15’, and was not clustered according to geographical origin. At the genetic similarity coefficient of 0.36, group I can be divided into 7 subclasses. Based on the principle of identifying the most varieties with the least primers, C59, S25, C33 and S151 were used to distinguish all varieties tested, and the digital fingerprints and molecular identity cards based on SSR markers were constructed. The genetic diversity of potato germplasm resources can be evaluated by using SSR markers, and different potato varieties（lines）can be accurately identified and traced by molecular identification cards.

Key words: potato, varieties（lines）, genetic diversity, SSR markers, clustering analysis, fingerprints, molecular identity card

AN Miao, WANG Tong-tong, FU Yi-ting, XIA Jun-jun, PENG Suo-tang, DUAN Yong-hong. Genetic Diversity Analysis and Molecular Identity Card Construction by SSR Markers of 52 Solanum tuberosum L. Varieties（Lines）[J]. Biotechnology Bulletin, 2023, 39(12): 136-147.

Figures/Tables 9

References 41

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编号 Code	材料名称 Accession	编号 Code	材料名称 Accession
1	L1150-22	27	蓬勃2号 Pengbo 2
2	陇薯7号 Longshu 7	28	晋薯16号 Jinshu 16
3	陇薯10号 Longshu 10	29	228
4	陇薯14号 Longshu 14	30	冀张薯8号 Jizhangshu 8
5	陇薯20号 Longshu 20	31	北方013 Beifang 013
6	陇薯19号 Longshu 19	32	北方016 Beifang 016
7	石薯6号 Shishu 6	33	青薯10号 Qingshu 10
8	青薯9号Qingshu 9	34	石薯1号 Shishu 1
9	东农310 Dongnong 310	35	石薯3号 Shishu 3
10	同薯20号 Tongshu 20	36	荷兰806 Holland 806
11	中薯306 Zhongshu 306	37	EV
12	克新25 Kexin 25	38	云薯6号 Yunshu 6
13	中薯9号 Zhongshu 9	39	实验1号 Shiyan 1
14	476	40	希森6号 Xisen 6
15	晋薯15号 Jinshu 15	41	中加2号 Zhongjia 2
16	新大坪 Xindaping	42	118
17	大同里外黄 Datongiwaihuang	43	V7
18	民丰红 Minfenghong	44	V9
19	鄂薯3号 Eshu 3	45	雪川红 Xuechuanhong
20	克新23号 Kexin 23	46	黑金刚 Heijingang
21	同薯29号 Tongshu 29	47	紫玫瑰 Zimeigui
22	川引2号 Chuanyin 2	48	丽薯6号 Lishu 6
23	京张薯1号 Jingzhangshu 1	49	冀张薯20号 Jizhangshu 20
24	京张薯3号 Jingzhangshu 3	50	克新13 Kexin 13
25	实验2号 Shiyan 2	51	荷兰14 Holland 14
26	蓬勃1号 Pengbo 1	52	冀张薯12号 Jizhangshu 12

编号 Code	材料名称 Accession	编号 Code	材料名称 Accession
1	L1150-22	27	蓬勃2号 Pengbo 2
2	陇薯7号 Longshu 7	28	晋薯16号 Jinshu 16
3	陇薯10号 Longshu 10	29	228
4	陇薯14号 Longshu 14	30	冀张薯8号 Jizhangshu 8
5	陇薯20号 Longshu 20	31	北方013 Beifang 013
6	陇薯19号 Longshu 19	32	北方016 Beifang 016
7	石薯6号 Shishu 6	33	青薯10号 Qingshu 10
8	青薯9号Qingshu 9	34	石薯1号 Shishu 1
9	东农310 Dongnong 310	35	石薯3号 Shishu 3
10	同薯20号 Tongshu 20	36	荷兰806 Holland 806
11	中薯306 Zhongshu 306	37	EV
12	克新25 Kexin 25	38	云薯6号 Yunshu 6
13	中薯9号 Zhongshu 9	39	实验1号 Shiyan 1
14	476	40	希森6号 Xisen 6
15	晋薯15号 Jinshu 15	41	中加2号 Zhongjia 2
16	新大坪 Xindaping	42	118
17	大同里外黄 Datongiwaihuang	43	V7
18	民丰红 Minfenghong	44	V9
19	鄂薯3号 Eshu 3	45	雪川红 Xuechuanhong
20	克新23号 Kexin 23	46	黑金刚 Heijingang
21	同薯29号 Tongshu 29	47	紫玫瑰 Zimeigui
22	川引2号 Chuanyin 2	48	丽薯6号 Lishu 6
23	京张薯1号 Jingzhangshu 1	49	冀张薯20号 Jizhangshu 20
24	京张薯3号 Jingzhangshu 3	50	克新13 Kexin 13
25	实验2号 Shiyan 2	51	荷兰14 Holland 14
26	蓬勃1号 Pengbo 1	52	冀张薯12号 Jizhangshu 12

序号No.	引物Primer	正向引物Forword primer（5'-3'）	反向引物Reverse primer（5'-3'）
1	C33	TCCCCTTACCCCTCTTCAAAT	AACCCGTGTCAACTTCTGCT
2	C59	TCATCACAACGTGACCCCA	GGGCTTGAATGATGTGAAGCTC
3	S25	AGAGATCGATGTAAAACACGT	GTGGCATTTTGATGGATT
4	C42	AGGCAAACCCCGTAAATCTT	TCTTCCTCACTCGTCCCACT
5	S151	GCTGCTAAACACTCAAGCAGAA	CAACTACAAGATTCCATCCACAC
6	STM-2022	GCGTCAGCGATTTCAGTACTA	TTCAGTCAACTCCTGTTGCG
7	C54	TGGCTGCAGAAATCCAAGATG	TGAAACCACTGGGAGGACTT
8	C58	GCTGCTAAACACTCAAGCAGAA	CAACTACAAGATTCCATCCACAC
9	C3	CCGGAGAATTAGACGATGA	TTTATGGGGAGCAGTTGAGG
10	S153	CCCTTACCCCTCTTCAATC	TCTCTTCTCAGCTGCCGTCT
11	UGP	TGGGGTTCCATCAAAC	GAAACTGCTGCCGGTGC
12	STM1016	TTCTGATTTCATGCATGTTTCC	ATGCTTGCCATGTGATGTGT
13	Patain	TGGTCTGGTGCATTAGAAAAAA	CAACCAACAAGGTAAATGGTACC
14	S170	CGCAAATCTTCATCCGATTC	TCCGGCGGATAATACTTGTT
15	S118	AGAGATCGATGTAAAACACGT	GTGGCATTTTGATGCATT
16	STM0030	AGAGATCGATGTAAAACACGT	GTGGCATTTTGATGGATT
17	C57	AGCTGGCTGACCCTGAACTC	GACAAAATTACAGGAACTGCAAA
18	S148	CAGCAAAATCAGAACCCGAT	GGATCATCAAATTCACCGCT
19	STG0016	AGCTGCTCAGCATCAAGAGA	ACCACCTCAGGCACTTCATC
20	S174	TGAGGGTTTTCAGAAAGGGA	CATCCTTGCAACAACCTCCT
21	StI0003	ACCATCCACCATGTCAATGC	CTCATGGATGGTGTCATTGG
22	SSI	TCTCTTGACACGTGTCACTGAAAC	TCACCGATTACAGTAGGCAAGAGA
23	S189	CCTTGTAGTACAGCAGTGGTC	TCCGCCAAGACTGATGCA
24	STM0014	CAGTCTTCAGCCCATAGG	TAAACAATGGTAGACAAGACAAA
25	STM2020	GCGTCAGCGATTTCAGTACTA	TTCAGTCAACTCCTGTTGCG

序号No.	引物Primer	正向引物Forword primer（5'-3'）	反向引物Reverse primer（5'-3'）
1	C33	TCCCCTTACCCCTCTTCAAAT	AACCCGTGTCAACTTCTGCT
2	C59	TCATCACAACGTGACCCCA	GGGCTTGAATGATGTGAAGCTC
3	S25	AGAGATCGATGTAAAACACGT	GTGGCATTTTGATGGATT
4	C42	AGGCAAACCCCGTAAATCTT	TCTTCCTCACTCGTCCCACT
5	S151	GCTGCTAAACACTCAAGCAGAA	CAACTACAAGATTCCATCCACAC
6	STM-2022	GCGTCAGCGATTTCAGTACTA	TTCAGTCAACTCCTGTTGCG
7	C54	TGGCTGCAGAAATCCAAGATG	TGAAACCACTGGGAGGACTT
8	C58	GCTGCTAAACACTCAAGCAGAA	CAACTACAAGATTCCATCCACAC
9	C3	CCGGAGAATTAGACGATGA	TTTATGGGGAGCAGTTGAGG
10	S153	CCCTTACCCCTCTTCAATC	TCTCTTCTCAGCTGCCGTCT
11	UGP	TGGGGTTCCATCAAAC	GAAACTGCTGCCGGTGC
12	STM1016	TTCTGATTTCATGCATGTTTCC	ATGCTTGCCATGTGATGTGT
13	Patain	TGGTCTGGTGCATTAGAAAAAA	CAACCAACAAGGTAAATGGTACC
14	S170	CGCAAATCTTCATCCGATTC	TCCGGCGGATAATACTTGTT
15	S118	AGAGATCGATGTAAAACACGT	GTGGCATTTTGATGCATT
16	STM0030	AGAGATCGATGTAAAACACGT	GTGGCATTTTGATGGATT
17	C57	AGCTGGCTGACCCTGAACTC	GACAAAATTACAGGAACTGCAAA
18	S148	CAGCAAAATCAGAACCCGAT	GGATCATCAAATTCACCGCT
19	STG0016	AGCTGCTCAGCATCAAGAGA	ACCACCTCAGGCACTTCATC
20	S174	TGAGGGTTTTCAGAAAGGGA	CATCCTTGCAACAACCTCCT
21	StI0003	ACCATCCACCATGTCAATGC	CTCATGGATGGTGTCATTGG
22	SSI	TCTCTTGACACGTGTCACTGAAAC	TCACCGATTACAGTAGGCAAGAGA
23	S189	CCTTGTAGTACAGCAGTGGTC	TCCGCCAAGACTGATGCA
24	STM0014	CAGTCTTCAGCCCATAGG	TAAACAATGGTAGACAAGACAAA
25	STM2020	GCGTCAGCGATTTCAGTACTA	TTCAGTCAACTCCTGTTGCG

序号 No.	引物名称 Primer name	等位位点 Alleles	多态性位点 Polymorphic alleles	多态位点百分数 Percentage of polymorphic bands/%	PIC	Na	Ne	H	I
1	C33	18	14	77.78	0.08	2.00	1.12	0.08	0.16
2	C59	26	26	100.00	0.17	2.00	1.24	0.17	0.29
3	S25	20	14	70.00	0.11	2.00	1.15	0.11	0.21
4	C42	20	10	50.00	0.11	2.00	1.14	0.11	0.20
5	S151	8	5	62.50	0.02	2.00	1.18	0.11	0.19
6	STM-2022	13	11	84.62	0.24	2.00	1.38	0.24	0.39
7	C54	9	7	77.78	0.12	2.00	1.16	0.12	0.21
8	C58	7	5	71.43	0.14	1.85	1.20	0.12	0.20
9	C3	4	2	50.00	0.30	1.50	1.22	0.15	0.24
10	S153	21	14	66.67	0.18	1.95	1.25	0.17	0.29
11	UGP	13	5	38.46	0.13	2.00	1.19	0.13	0.23
12	STM1016	7	2	28.57	0.20	2.00	1.34	0.20	0.32
13	Patain	5	3	60.00	0.06	2.00	1.07	0.06	0.13
14	S170	18	6	33.33	0.21	2.00	1.21	0.15	0.26
15	S118	14	10	71.43	0.08	2.00	1.31	0.20	0.32
16	STM0030	14	13	92.86	0.17	2.00	1.24	0.12	0.17
合计 Total		217	147	67.74	-	-	-	-	-
平均值Mean		13.56	9.19	64.71	0.15	1.96	1.21	0.14	0.24