Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 81-94.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0011
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ZHANG Mei-yu1,2(), ZHAO Yu-bin3, WANG Ling-yun3, SONG Yuan-da1,2, ZHAO Xin-he1,2(), REN Xiao-jie1,2()
Received:
2024-01-04
Online:
2024-06-26
Published:
2024-05-15
Contact:
ZHAO Xin-he, REN Xiao-jie
E-mail:zmeiyu1118@163.com;zhaoxinhe@sdut.edu.cn;renxiaojie@sdut.edu.cn
ZHANG Mei-yu, ZHAO Yu-bin, WANG Ling-yun, SONG Yuan-da, ZHAO Xin-he, REN Xiao-jie. Research Progress in the Production of Functional Fatty Acid DHA by Microalga Thraustochytrids[J]. Biotechnology Bulletin, 2024, 40(6): 81-94.
菌株 Bacterial species | 来源 Source | 产油特性 Oil production characteristics | 参考文献Reference |
---|---|---|---|
橙黄壶菌ZJWZ-7 Aurantiochytrium ZJWZ-7 | 中国沿海红树林 Chinese coastal mangroves | DHA和饱和脂肪酸分别达到1.66 g/L和1.68 g/L DHA and saturated fatty acids reach 1.66 g/L and 1.68 g/L respectively | [ |
裂殖壶菌LU310 Schizochytrium sp. LU310 | 温州红树林 Wenzhou angroves | DHA产量高达24.74 g/L DHA production reaches as high as 24.74 g/L | [ |
裂殖壶菌ABC101 Schizochytrium sp. ABC101 | 海水中 Seawater | DHA达到11.0 g/L DHA reaches 11.0 g/L | [ |
裂殖壶菌S31 Schizochytrium sp. S31 | 美国加利福尼亚河 California River, USA | DHA产率高达300 mg/(L·d) DHA productivity reaches 300 mg/(L·d) | [ |
橙黄壶菌TL18 Aurantiochytrium sp. TL18 | 英国水域 British waters | DHA产量占多不饱和脂肪酸的83% DHA production accounts for 83% of polyunsaturated fatty acids | [ |
橙黄壶菌YB-05 Aurantiochytrium sp. YB-05 | 沙特阿拉伯红海沿岸的腐叶中Decayed leaves from the Red Sea in Saudi Arabia | DHA含量为24.85% DHA content is 24.85% | [ |
橙黄壶菌SW1 Aurantiochytrium sp. SW1 | 马来西亚 Malaysia | DHA产量达到3.6 g/L DHA production reaches 3.6 g/L | [ |
Table 1 High oil-producing strains selected from nature
菌株 Bacterial species | 来源 Source | 产油特性 Oil production characteristics | 参考文献Reference |
---|---|---|---|
橙黄壶菌ZJWZ-7 Aurantiochytrium ZJWZ-7 | 中国沿海红树林 Chinese coastal mangroves | DHA和饱和脂肪酸分别达到1.66 g/L和1.68 g/L DHA and saturated fatty acids reach 1.66 g/L and 1.68 g/L respectively | [ |
裂殖壶菌LU310 Schizochytrium sp. LU310 | 温州红树林 Wenzhou angroves | DHA产量高达24.74 g/L DHA production reaches as high as 24.74 g/L | [ |
裂殖壶菌ABC101 Schizochytrium sp. ABC101 | 海水中 Seawater | DHA达到11.0 g/L DHA reaches 11.0 g/L | [ |
裂殖壶菌S31 Schizochytrium sp. S31 | 美国加利福尼亚河 California River, USA | DHA产率高达300 mg/(L·d) DHA productivity reaches 300 mg/(L·d) | [ |
橙黄壶菌TL18 Aurantiochytrium sp. TL18 | 英国水域 British waters | DHA产量占多不饱和脂肪酸的83% DHA production accounts for 83% of polyunsaturated fatty acids | [ |
橙黄壶菌YB-05 Aurantiochytrium sp. YB-05 | 沙特阿拉伯红海沿岸的腐叶中Decayed leaves from the Red Sea in Saudi Arabia | DHA含量为24.85% DHA content is 24.85% | [ |
橙黄壶菌SW1 Aurantiochytrium sp. SW1 | 马来西亚 Malaysia | DHA产量达到3.6 g/L DHA production reaches 3.6 g/L | [ |
菌株 Bacterial species | 突变方法 Mutation method | 结果 Result | 参考文献Reference |
---|---|---|---|
橙黄壶菌CG Aurantiochytrium sp.CGMCC 6208 | 重离子诱变 Heavy ion mutagenesis | DHA产量由21 g/L提高到27 g/L DHA production increased from 21 g/L to 27 g/L | [ |
橙黄壶菌SW1 Aurantiochytrium sp. SW1 | ARTP | 生物量、油脂和DHA产量分别提高30%、65%和80% Biomass, lipid content, and DHA production increased by 30%, 65%, and 80%, respectively | [ |
裂殖壶菌B4D1 Schizochytrium sp. B4D1 | 紫外诱变 UV mutagenesis | DHA含量达到总油脂的41.24%,提高了11.70% The DHA content reached 41.24% of total lipids, increasing by 11.70% | [ |
裂殖壶菌SR21 Schizochytrium limacinum SR21 | ARTP | DHA产量达到6. 59 g/L,提高了46.12% The DHA production reached 6.59 g/L, representing a 46.12% increase | [ |
裂殖壶菌ATCC20888 Schizochytrium sp. ATCC20888 | 低能离子诱变 Low-energy ion mutagenesis | 生物量为48.01 g/L,DHA产量最高为6.52 g/L Biomass was 48.01 g/L, with a maximum DHA production of 6.52 g/L | [ |
橙黄壶菌PKU#SW7 Aurantiochytrium sp. PKU#SW7 | 紫外诱变 UV mutagenesis | 生物量为6.62 g/L,DHA在脂肪酸中所占比例由29.97%增加到33.43% Biomass was 6.62 g/L, and the proportion of DHA in fatty acids increased from 29.97% to 33.43% | [ |
裂殖壶菌M209059 Schizochytrium sp. M209059 | NTG结合紫外诱变 NTG combined with UV mutagenesis | DHA占总脂肪酸的比例达到56.22%,提高了38.88% The proportion of DHA in total fatty acids reached 56.22%, representing an increase of 38.88% | [ |
裂殖壶菌 Schizochytrium sp. | 甲烷磺酸乙酯(EMS)结合紫外诱变 EMS combined with UV mutagenesis | 脂质含量和DHA产量分别提高了8.53%和21.58% Lipid content and DHA production increased by 8.53% and 21.58%, respectively | [ |
裂殖壶菌31 Schizochytrium sp. 31 | EMS和ARTP复合诱变 EMS and ARTP combined mutagenesis | DHA产量达到7.2 g/L,比亲本菌株提高35.6% The DHA production reached 7.2 g/L, representing a 35.6% increase compared to the parental strain | [ |
裂殖壶菌ALE70 Schizochytrium sp. ALE70 | 耐热性ALE Heat-resistant ALE | 油脂产量和DHA产量分别提高了4.31倍和4.33倍 The lipid production and DHA production increased by 4.31-fold and 4.33-fold, respectively | [ |
裂殖壶菌HX-308 Schizochytrium sp. HX-308 | 高氧ALE High-oxygen ALE | 细胞干重达到84.34 g/L,DHA产量为26.40 g/L Cell dry weight reached 84.34 g/L, and DHA production was 26.40 g/L | [ |
低温与高盐度双因子协同ALE Synergistic ALE of Low Temperature and High Salinity | DHA产量为38.12 g/L,比亲本菌株高57.52% The DHA production is 38.12 g/L, which is 57.52% higher than the parental strain | [ | |
裂殖壶菌 Schizochytrium limacinum B4D1 | 低温ALE Low-temperature ALE | DHA含量提高了11.22% DHA content increased by 11.22% | [ |
Table 2 Research results on the improvement of high oil-producing algal strains
菌株 Bacterial species | 突变方法 Mutation method | 结果 Result | 参考文献Reference |
---|---|---|---|
橙黄壶菌CG Aurantiochytrium sp.CGMCC 6208 | 重离子诱变 Heavy ion mutagenesis | DHA产量由21 g/L提高到27 g/L DHA production increased from 21 g/L to 27 g/L | [ |
橙黄壶菌SW1 Aurantiochytrium sp. SW1 | ARTP | 生物量、油脂和DHA产量分别提高30%、65%和80% Biomass, lipid content, and DHA production increased by 30%, 65%, and 80%, respectively | [ |
裂殖壶菌B4D1 Schizochytrium sp. B4D1 | 紫外诱变 UV mutagenesis | DHA含量达到总油脂的41.24%,提高了11.70% The DHA content reached 41.24% of total lipids, increasing by 11.70% | [ |
裂殖壶菌SR21 Schizochytrium limacinum SR21 | ARTP | DHA产量达到6. 59 g/L,提高了46.12% The DHA production reached 6.59 g/L, representing a 46.12% increase | [ |
裂殖壶菌ATCC20888 Schizochytrium sp. ATCC20888 | 低能离子诱变 Low-energy ion mutagenesis | 生物量为48.01 g/L,DHA产量最高为6.52 g/L Biomass was 48.01 g/L, with a maximum DHA production of 6.52 g/L | [ |
橙黄壶菌PKU#SW7 Aurantiochytrium sp. PKU#SW7 | 紫外诱变 UV mutagenesis | 生物量为6.62 g/L,DHA在脂肪酸中所占比例由29.97%增加到33.43% Biomass was 6.62 g/L, and the proportion of DHA in fatty acids increased from 29.97% to 33.43% | [ |
裂殖壶菌M209059 Schizochytrium sp. M209059 | NTG结合紫外诱变 NTG combined with UV mutagenesis | DHA占总脂肪酸的比例达到56.22%,提高了38.88% The proportion of DHA in total fatty acids reached 56.22%, representing an increase of 38.88% | [ |
裂殖壶菌 Schizochytrium sp. | 甲烷磺酸乙酯(EMS)结合紫外诱变 EMS combined with UV mutagenesis | 脂质含量和DHA产量分别提高了8.53%和21.58% Lipid content and DHA production increased by 8.53% and 21.58%, respectively | [ |
裂殖壶菌31 Schizochytrium sp. 31 | EMS和ARTP复合诱变 EMS and ARTP combined mutagenesis | DHA产量达到7.2 g/L,比亲本菌株提高35.6% The DHA production reached 7.2 g/L, representing a 35.6% increase compared to the parental strain | [ |
裂殖壶菌ALE70 Schizochytrium sp. ALE70 | 耐热性ALE Heat-resistant ALE | 油脂产量和DHA产量分别提高了4.31倍和4.33倍 The lipid production and DHA production increased by 4.31-fold and 4.33-fold, respectively | [ |
裂殖壶菌HX-308 Schizochytrium sp. HX-308 | 高氧ALE High-oxygen ALE | 细胞干重达到84.34 g/L,DHA产量为26.40 g/L Cell dry weight reached 84.34 g/L, and DHA production was 26.40 g/L | [ |
低温与高盐度双因子协同ALE Synergistic ALE of Low Temperature and High Salinity | DHA产量为38.12 g/L,比亲本菌株高57.52% The DHA production is 38.12 g/L, which is 57.52% higher than the parental strain | [ | |
裂殖壶菌 Schizochytrium limacinum B4D1 | 低温ALE Low-temperature ALE | DHA含量提高了11.22% DHA content increased by 11.22% | [ |
菌株 Bacterial species | 基因 Gene | 电击参数 Electric shock parameters | 结果 Result | 参考文献 Reference |
---|---|---|---|---|
裂殖壶菌S31 Schizochytrium sp. S31 | 过表达来自隐甲藻的苹果酸酶和高山被孢霉的ELO3基因 Overexpression of malate synthase from Thalassiosira pseudonana and ELO3 gene from Mortierella alpina | 2 000 V, 50 μF, 200 Ω | DHA产量为3.54 g/L,占细胞干重的26.70% DHA production reached 3.54 g/L, accounting for 26.70% of cell dry weight | [ |
裂殖壶菌ATCC 20888 Schizochytrium sp. ATCC 20888 | 过表达硫氧还蛋白还原酶、乙醛脱氢酶、谷胱甘肽过氧化物酶和葡萄糖-6 -磷酸脱氢酶的基因 Overexpression of genes encoding thioredoxin reductase, aldehyde dehydrogenase, glutathione peroxidase, and glucose-6-phosphate dehydrogenase | 1 500 V, 200 Ω, 50 μF | DHA产量达到13.3 g/L DHA production reached 13.3 g/L | [ |
橙黄壶菌SZU445 Aurantiochytrium sp. SZU445 | 敲除2,4 -二烯基辅酶A还原酶基因 Knockout of the gene encoding 2,4-dienoyl-CoA reductase | 1 500 V, 50 μF, 200 Ω | DHA产量占总脂肪酸的53.97% DHA production accounted for 53.97% of total fatty acids | [ |
裂殖壶菌H016 Schizochytrium sp. H016 | 过表达内源葡萄糖- 6 -磷酸脱氢酶基因 Overexpression of endogenous glucose-6-phosphate dehydrogenase gene | 1 400 V, 200 Ω, 50 μF | DHA产量达到17.01 g/L,提高了31.47% The DHA production reached 17.01 g/L, representing a 31.47% increase | [ |
裂殖壶菌TIO110 Schizochytrium sp. TIO1101 | 过表达大肠杆菌乙酰辅酶A合成酶基因 Overexpression of Escherichia coli acetyl-CoA synthase gene | 2 100 V, 4.5 ms | 生物量和脂肪酸比例分别增加了29.9%和11.3% Biomass and fatty acid proportion increased by 29.9% and 11.3%, respectively | [ |
裂殖壶菌ATCC1381 Schizochytrium sp. ATCC1381 | 过表达丙二酰辅酶A转移酶 Overexpression of acyltransferase | 7.5 kV/cm, 50 μF, 50 Ω | 总脂产量提高到110.5 g/L,比野生菌株提高39.6%,DHA产量为47.39 g/L Total lipid production increased to 110.5 g/L, a 39.6% increase compared to the wild-type strain, with DHA production at 47.39 g/L | [ |
裂殖壶菌PKU#Mn42 Schizochytrium sp. PKU#Mn42 | 抗氧化基因超氧化物歧化酶 Antioxidant gene superoxide dismutase | 1 000 V, 50 μF, 500 Ω | 多不饱和脂肪酸(PUFA)产量增加1.37 倍 Polyunsaturated fatty acids(PUFA)production increased by 1.37 times | [ |
Table 3 Research outcomes of high DHA production in strains achieved by electric conversion method
菌株 Bacterial species | 基因 Gene | 电击参数 Electric shock parameters | 结果 Result | 参考文献 Reference |
---|---|---|---|---|
裂殖壶菌S31 Schizochytrium sp. S31 | 过表达来自隐甲藻的苹果酸酶和高山被孢霉的ELO3基因 Overexpression of malate synthase from Thalassiosira pseudonana and ELO3 gene from Mortierella alpina | 2 000 V, 50 μF, 200 Ω | DHA产量为3.54 g/L,占细胞干重的26.70% DHA production reached 3.54 g/L, accounting for 26.70% of cell dry weight | [ |
裂殖壶菌ATCC 20888 Schizochytrium sp. ATCC 20888 | 过表达硫氧还蛋白还原酶、乙醛脱氢酶、谷胱甘肽过氧化物酶和葡萄糖-6 -磷酸脱氢酶的基因 Overexpression of genes encoding thioredoxin reductase, aldehyde dehydrogenase, glutathione peroxidase, and glucose-6-phosphate dehydrogenase | 1 500 V, 200 Ω, 50 μF | DHA产量达到13.3 g/L DHA production reached 13.3 g/L | [ |
橙黄壶菌SZU445 Aurantiochytrium sp. SZU445 | 敲除2,4 -二烯基辅酶A还原酶基因 Knockout of the gene encoding 2,4-dienoyl-CoA reductase | 1 500 V, 50 μF, 200 Ω | DHA产量占总脂肪酸的53.97% DHA production accounted for 53.97% of total fatty acids | [ |
裂殖壶菌H016 Schizochytrium sp. H016 | 过表达内源葡萄糖- 6 -磷酸脱氢酶基因 Overexpression of endogenous glucose-6-phosphate dehydrogenase gene | 1 400 V, 200 Ω, 50 μF | DHA产量达到17.01 g/L,提高了31.47% The DHA production reached 17.01 g/L, representing a 31.47% increase | [ |
裂殖壶菌TIO110 Schizochytrium sp. TIO1101 | 过表达大肠杆菌乙酰辅酶A合成酶基因 Overexpression of Escherichia coli acetyl-CoA synthase gene | 2 100 V, 4.5 ms | 生物量和脂肪酸比例分别增加了29.9%和11.3% Biomass and fatty acid proportion increased by 29.9% and 11.3%, respectively | [ |
裂殖壶菌ATCC1381 Schizochytrium sp. ATCC1381 | 过表达丙二酰辅酶A转移酶 Overexpression of acyltransferase | 7.5 kV/cm, 50 μF, 50 Ω | 总脂产量提高到110.5 g/L,比野生菌株提高39.6%,DHA产量为47.39 g/L Total lipid production increased to 110.5 g/L, a 39.6% increase compared to the wild-type strain, with DHA production at 47.39 g/L | [ |
裂殖壶菌PKU#Mn42 Schizochytrium sp. PKU#Mn42 | 抗氧化基因超氧化物歧化酶 Antioxidant gene superoxide dismutase | 1 000 V, 50 μF, 500 Ω | 多不饱和脂肪酸(PUFA)产量增加1.37 倍 Polyunsaturated fatty acids(PUFA)production increased by 1.37 times | [ |
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