Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 99-107.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0225
Previous Articles Next Articles
HE Yu-bing1,2(), FU Zhen-hao1,2, LI Ren-han1,2, LIU Xiu-xia1,2, LIU Chun-li1,2, YANG Yan-kun1,2, LI Ye1,2(), BAI Zhong-hu1,2()
Received:
2024-03-08
Online:
2024-07-26
Published:
2024-07-30
Contact:
LI Ye, BAI Zhong-hu
E-mail:6210201011@stu.jiangnan.edu.cn;yeli0622@jiangnan.edu.cn;baizhonghu@jiangnan.edu.cn
HE Yu-bing, FU Zhen-hao, LI Ren-han, LIU Xiu-xia, LIU Chun-li, YANG Yan-kun, LI Ye, BAI Zhong-hu. Efficient Biosynthesis of 2-Naphthaleneethanol in Metabolically Engineered Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2024, 40(7): 99-107.
名称Name | 序列Sequence(5'-3') |
---|---|
ARO8_F | GCATCGTCTCATCGGTCTCATATGACTTTACCTGAATCAAAAGACTTTTCTT |
ARO8_R | ATGCCGTCTCAGGTCTCAGGATCCTTTGGAAATACCAAATTCTTCGTATAAAGT |
ARO9_F | GCATCGTCTCATCGGTCTCATATGACTGCTGGTTCTGCC |
ARO9_R | ATGCCGTCTCAGGTCTCAGGATCCACTTTTATAGTTGTCAAAAAATTCTTTTATGCC |
ARO10_F | GCATCGTCTCATCGGTCTCATATGGCACCTGTTACAATTGAAAAGT |
ARO10_R | ATGCCGTCTCAGGTCTCAGGATCCTTTTTTATTTCTTTTAAGTGCCGCTGC |
PDC1_F | GCATCGTCTCATCGGTCTCATATGTCTGAAATTACTTTGGGTAAATATTTGT |
PDC1_R | ATGCCGTCTCAGGTCTCAGGATCCTTGCTTAGCGTTGGTAGCAG |
PDC5_F | GCATCGTCTCATCGGTCTCATATGTCTGAAATAACCTTAGGTAAATATTTATTTGAA |
PDC5_R | ATGCCGTCTCAGGTCTCAGGATCCTTGTTTAGCGTTAGTAGCGGC |
PDC6_F | GCATCGTCTCATCGGTCTCATATGTCTGAAATTACTCTTGGAAAATACTT |
PDC6_R | ATGCCGTCTCAGGTCTCAGGATCCTTGTTTGGCATTTGTAGCGG |
HO5'_F | CACATCATTTTCGTGGATCC |
HO5'_R | ACAGCGATGGAACTTACGGC |
HO3'_F | TATCGTGTTGCATCTGCGGC |
HO3'_R | CTTTGGACTTAAAATGGCGT |
Table 1 Primers used in this study
名称Name | 序列Sequence(5'-3') |
---|---|
ARO8_F | GCATCGTCTCATCGGTCTCATATGACTTTACCTGAATCAAAAGACTTTTCTT |
ARO8_R | ATGCCGTCTCAGGTCTCAGGATCCTTTGGAAATACCAAATTCTTCGTATAAAGT |
ARO9_F | GCATCGTCTCATCGGTCTCATATGACTGCTGGTTCTGCC |
ARO9_R | ATGCCGTCTCAGGTCTCAGGATCCACTTTTATAGTTGTCAAAAAATTCTTTTATGCC |
ARO10_F | GCATCGTCTCATCGGTCTCATATGGCACCTGTTACAATTGAAAAGT |
ARO10_R | ATGCCGTCTCAGGTCTCAGGATCCTTTTTTATTTCTTTTAAGTGCCGCTGC |
PDC1_F | GCATCGTCTCATCGGTCTCATATGTCTGAAATTACTTTGGGTAAATATTTGT |
PDC1_R | ATGCCGTCTCAGGTCTCAGGATCCTTGCTTAGCGTTGGTAGCAG |
PDC5_F | GCATCGTCTCATCGGTCTCATATGTCTGAAATAACCTTAGGTAAATATTTATTTGAA |
PDC5_R | ATGCCGTCTCAGGTCTCAGGATCCTTGTTTAGCGTTAGTAGCGGC |
PDC6_F | GCATCGTCTCATCGGTCTCATATGTCTGAAATTACTCTTGGAAAATACTT |
PDC6_R | ATGCCGTCTCAGGTCTCAGGATCCTTGTTTGGCATTTGTAGCGG |
HO5'_F | CACATCATTTTCGTGGATCC |
HO5'_R | ACAGCGATGGAACTTACGGC |
HO3'_F | TATCGTGTTGCATCTGCGGC |
HO3'_R | CTTTGGACTTAAAATGGCGT |
名称 Name | 携带基因/特征 Characteristics of carried gene | 大肠杆菌抗性标记 E. coli marker | 部件类型/使用的部件 Type/type used | 来源 Origin |
---|---|---|---|---|
pYTK001 | Part plasmid entry vector | CamR | entry vector | Lee等[ |
pYTK002 | ConLS | CamR | 1 | Lee等[ |
pYTK003 | ConL1 | CamR | 1 | Lee等[ |
pYTK004 | ConL2 | CamR | 1 | Lee等[ |
pYTK009 | pTDH3 | CamR | 2 | Lee等[ |
pYTK011 | pPGK1 | CamR | 2 | Lee等[ |
pYTK048 | Spacer | CamR | 234 | Lee等[ |
pYTK051 | tENO1 | CamR | 4 | Lee等[ |
pYTK052 | tSSA1 | CamR | 4 | Lee等[ |
pYTK067 | ConR1 | CamR | 5 | Lee等[ |
pYTK068 | ConR2 | CamR | 5 | Lee等[ |
pYTK072 | ConRE | CamR | 5 | Lee等[ |
pYTK095 | AmpR-ColE1 | AmpR | 678 | Lee等[ |
pBL602 | Integration(HO-loc)HIS3 GFP Dropout | KanR | 15678 | 实验室保藏 |
pBL617 | ARO8 | CamR | 3 | 实验室保藏 |
pBL618 | ARO9 | CamR | 3 | 实验室保藏 |
pBL621 | ARO10 | CamR | 3 | 实验室保藏 |
pBL623 | PDC1 | CamR | 3 | 实验室保藏 |
pBL624 | PDC5 | CamR | 3 | 实验室保藏 |
pBL625 | PDC6 | CamR | 3 | 实验室保藏 |
pBL883 | ConL1-Spacer-ConRE | AmpR | pYTK003, pYTK048, pYTK072, pYTK095 | 本研究构建 |
pBL884 | ConL2-Spacer-ConRE | AmpR | pYTK004, pYTK048, pYTK072, pYTK096 | 本研究构建 |
pBL885 | ConLS-pPGK1-ARO8-tENO1-ConR1 | AmpR | pYTK002, pYTK011, pYTK051, pYTK067, pYTK095, pBL617 | 本研究构建 |
pBL886 | ConLS-pPGK1-ARO9-tENO1-ConR1 | AmpR | pYTK002, pYTK011, pYTK051, pYTK067, pYTK095, pBL618 | 本研究构建 |
pBL889 | ConL1-pTDH3-ARO10-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL621 | 本研究构建 |
pBL890 | ConL1-pTDH3-PDC1-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL623 | 本研究构建 |
pBL891 | ConL1-pTDH3-PDC5-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL624 | 本研究构建 |
pBL892 | ConL1-pTDH3-PDC6-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL625 | 本研究构建 |
pBL909 | Integration(HO-loc)HIS3 pPGK1-ARO8-tENO1 | KanR | pBL602, pBL883, pBL885 | 本研究构建 |
pBL910 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 | KanR | pBL602, pBL883, pBL886 | 本研究构建 |
pBL961 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-ARO10-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL889 | 本研究构建 |
pBL962 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-PDC1-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL890 | 本研究构建 |
pBL963 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-PDC5-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL891 | 本研究构建 |
pBL964 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-PDC6-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL892 | 本研究构建 |
Table 2 Plasmids used in this study
名称 Name | 携带基因/特征 Characteristics of carried gene | 大肠杆菌抗性标记 E. coli marker | 部件类型/使用的部件 Type/type used | 来源 Origin |
---|---|---|---|---|
pYTK001 | Part plasmid entry vector | CamR | entry vector | Lee等[ |
pYTK002 | ConLS | CamR | 1 | Lee等[ |
pYTK003 | ConL1 | CamR | 1 | Lee等[ |
pYTK004 | ConL2 | CamR | 1 | Lee等[ |
pYTK009 | pTDH3 | CamR | 2 | Lee等[ |
pYTK011 | pPGK1 | CamR | 2 | Lee等[ |
pYTK048 | Spacer | CamR | 234 | Lee等[ |
pYTK051 | tENO1 | CamR | 4 | Lee等[ |
pYTK052 | tSSA1 | CamR | 4 | Lee等[ |
pYTK067 | ConR1 | CamR | 5 | Lee等[ |
pYTK068 | ConR2 | CamR | 5 | Lee等[ |
pYTK072 | ConRE | CamR | 5 | Lee等[ |
pYTK095 | AmpR-ColE1 | AmpR | 678 | Lee等[ |
pBL602 | Integration(HO-loc)HIS3 GFP Dropout | KanR | 15678 | 实验室保藏 |
pBL617 | ARO8 | CamR | 3 | 实验室保藏 |
pBL618 | ARO9 | CamR | 3 | 实验室保藏 |
pBL621 | ARO10 | CamR | 3 | 实验室保藏 |
pBL623 | PDC1 | CamR | 3 | 实验室保藏 |
pBL624 | PDC5 | CamR | 3 | 实验室保藏 |
pBL625 | PDC6 | CamR | 3 | 实验室保藏 |
pBL883 | ConL1-Spacer-ConRE | AmpR | pYTK003, pYTK048, pYTK072, pYTK095 | 本研究构建 |
pBL884 | ConL2-Spacer-ConRE | AmpR | pYTK004, pYTK048, pYTK072, pYTK096 | 本研究构建 |
pBL885 | ConLS-pPGK1-ARO8-tENO1-ConR1 | AmpR | pYTK002, pYTK011, pYTK051, pYTK067, pYTK095, pBL617 | 本研究构建 |
pBL886 | ConLS-pPGK1-ARO9-tENO1-ConR1 | AmpR | pYTK002, pYTK011, pYTK051, pYTK067, pYTK095, pBL618 | 本研究构建 |
pBL889 | ConL1-pTDH3-ARO10-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL621 | 本研究构建 |
pBL890 | ConL1-pTDH3-PDC1-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL623 | 本研究构建 |
pBL891 | ConL1-pTDH3-PDC5-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL624 | 本研究构建 |
pBL892 | ConL1-pTDH3-PDC6-tSSA1-ConR2 | AmpR | pYTK003, pYTK009, pYTK052, pYTK068, pYTK095, pBL625 | 本研究构建 |
pBL909 | Integration(HO-loc)HIS3 pPGK1-ARO8-tENO1 | KanR | pBL602, pBL883, pBL885 | 本研究构建 |
pBL910 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 | KanR | pBL602, pBL883, pBL886 | 本研究构建 |
pBL961 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-ARO10-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL889 | 本研究构建 |
pBL962 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-PDC1-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL890 | 本研究构建 |
pBL963 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-PDC5-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL891 | 本研究构建 |
pBL964 | Integration(HO-loc)HIS3 pPGK1-ARO9-tENO1 pTDH3-PDC6-tSSA1 | KanR | pBL602, pBL884, pBL886, pBL892 | 本研究构建 |
名称Name | 基因型Genotype | 来源Origin |
---|---|---|
BY4741 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 | Brachmann等[ |
yBL_909 | [BY4741]Holoc::pBL_909 | 本研究构建 |
yBL_910 | [BY4741]Holoc::pBL_910 | 本研究构建 |
yBL_961 | [BY4741]Holoc::pBL_961 | 本研究构建 |
yBL_962 | [BY4741]Holoc::pBL_962 | 本研究构建 |
yBL_963 | [BY4741]Holoc::pBL_963 | 本研究构建 |
yBL_964 | [BY4741]Holoc::pBL_964 | 本研究构建 |
Table 3 Strains used in this study
名称Name | 基因型Genotype | 来源Origin |
---|---|---|
BY4741 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 | Brachmann等[ |
yBL_909 | [BY4741]Holoc::pBL_909 | 本研究构建 |
yBL_910 | [BY4741]Holoc::pBL_910 | 本研究构建 |
yBL_961 | [BY4741]Holoc::pBL_961 | 本研究构建 |
yBL_962 | [BY4741]Holoc::pBL_962 | 本研究构建 |
yBL_963 | [BY4741]Holoc::pBL_963 | 本研究构建 |
yBL_964 | [BY4741]Holoc::pBL_964 | 本研究构建 |
[1] | Li XM, Mi QL, Gao Q, et al. Antibacterial naphthalene derivatives from the fermentation products of the endophytic fungus Phomopsis fukushii[J]. Chem Nat Compd, 2021, 57(2): 293-296. |
[2] | 刘耀, 李枝芳, 张茂利. 萘环高分子在覆铜板中的应用[C]// 第二十届中国覆铜板技术研讨会论文集. 苏州, 2019: 228-235. |
[3] | Jiang WN, Zhao QL, Cheng WS, et al. CuI-mediated benzannulation of(ortho-arylethynyl)phenylenaminones to assemble α-aminonaphthalene derivatives[J]. Org Chem Front, 2021, 8(13): 3250-3254. |
[4] |
Lin B, Fan L, Ge JY, et al. A naphthalene-based fluorescent probe with a large Stokes shift for mitochondrial pH imaging[J]. Analyst, 2018, 143(20): 5054-5060.
doi: 10.1039/c8an01371c pmid: 30238115 |
[5] | Zeng CH, Xu ZY, Song C, et al. Naphthalene-based fluorescent probe for on-site detection of hydrazine in the environment[J]. J Hazard Mater, 2023, 445: 130415. |
[6] | Yokota K, Takeuchi S. Process for the preparation of 2-naphthylethanol, JP2006206533[P]. 2006-08-10. |
[7] | 刘学端. 微生物生态学驱动社会与经济“绿色高效发展”[J]. 微生物学通报, 2020, 47(9): 2681-2682. |
Liu XD. Microbial Ecology drives “Green and Efficient Development” of society and economy[J]. Microbiol China, 2020, 47(9): 2681-2682. | |
[8] | Ehrlich F. Über die Bedingungen der Fuselölbildung und über ihren Zusammenhang mit Dem Eiweißaufbau der Hefe[J]. Ber Dtsch Chem Ges, 1907, 40(1): 1027-1047. |
[9] | 于爱群, 庞亚如, 胡智慧, 等. 平台化学品短链支链脂肪酸和短链支链醇的微生物代谢工程[J]. 微生物学通报, 2018, 45(1): 173-180. |
Yu AQ, Pang YR, Hu ZH, et al. Advances in metabolic engineering for the microbial production of short branched-chain fatty acids and short branched-chain alcohols[J]. Microbiol China, 2018, 45(1): 173-180. | |
[10] | Iraqui I, Vissers S, Cartiaux M, et al. Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily[J]. Mol Gen Genet, 1998, 257(2): 238-248. |
[11] | Vuralhan Z, Luttik MAH, Tai SL, et al. Physiological characterization of the ARO10-dependent, broad-substrate-specificity 2-oxo acid decarboxylase activity of Saccharomyces cerevisiae[J]. Appl Environ Microbiol, 2005, 71(6): 3276-3284. |
[12] | 孙中贯, 刘琳, 王亚平, 等. 酿酒酵母高级醇代谢研究进展[J]. 生物工程学报, 2021, 37(2): 429-447. |
Sun ZG, Liu L, Wang YP, et al. Higher alcohols metabolism by Saccharomyces cerevisiae: a mini review[J]. Chin J Biotechnol, 2021, 37(2): 429-447. | |
[13] | Kondo T, Tezuka H, Ishii J, et al. Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae[J]. J Biotechnol, 2012, 159(1/2): 32-37. |
[14] |
孙井震, 何玙冰, 杨卫华, 等. 酿酒酵母过表达Ehrlich途径基因高效合成色醇[J]. 食品与发酵工业, 2022, 48(24): 16-23.
doi: 10.13995/j.cnki.11-1802/ts.031377 |
Sun JZ, He YB, Yang WH, et al. Efficient synthesis of indole-3-ethanol in Saccharomyces cerevisiae by overexpression of Ehrlich pathway genes[J]. Food Ferment Ind, 2022, 48(24): 16-23. | |
[15] | Chan WT, Verma CS, Lane DP, et al. A comparison and optimization of methods and factors affecting the transformation of Escherichia coli[J]. Biosci Rep, 2013, 33(6): e00086. |
[16] |
Lee ME, DeLoache WC, Cervantes B, et al. A highly characterized yeast toolkit for modular, multipart assembly[J]. ACS Synth Biol, 2015, 4(9): 975-986.
doi: 10.1021/sb500366v pmid: 25871405 |
[17] | Casini A, Storch M, Baldwin GS, et al. Bricks and blueprints: methods and standards for DNA assembly[J]. Nat Rev Mol Cell Biol, 2015, 16(9): 568-576. |
[18] |
Brachmann CB, Davies A, Cost GJ, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications[J]. Yeast, 1998, 14(2): 115-132.
doi: 10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2 pmid: 9483801 |
[19] |
Gietz RD, Schiestl RH. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method[J]. Nat Protoc, 2007, 2(1): 31-34.
doi: 10.1038/nprot.2007.13 pmid: 17401334 |
[20] |
Avalos JL, Fink GR, Stephanopoulos G. Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols[J]. Nat Biotechnol, 2013, 31(4): 335-341.
doi: 10.1038/nbt.2509 pmid: 23417095 |
[21] | Hassing EJ, de Groot PA, Marquenie VR, et al. Connecting central carbon and aromatic amino acid metabolisms to improve de novo 2-phenylethanol production in Saccharomyces cerevisiae[J]. Metab Eng, 2019, 56: 165-180. |
[22] | Jiang JJ, Yin H, Wang S, et al. Metabolic engineering of Saccharomyces cerevisiae for high-level production of salidroside from glucose[J]. J Agric Food Chem, 2018, 66(17): 4431-4438. |
[23] | Zhou R, Song QY, Xia HL, et al. Isolation and identification of non- Saccharomyces yeast producing 2-phenylethanol and study of the Ehrlich pathway and shikimate pathway[J]. J Fungi, 2023, 9(9): 878. |
[24] | 刘春筱, 夏媛媛, 齐丽娜, 等. 代谢工程改造大肠杆菌合成羟基酪醇[J]. 生物工程学报, 2021, 37(12): 4243-4253. |
Liu CX, Xia YY, Qi LN, et al. Metabolic engineering of Escherichia coli for production of hydroxytyrosol[J]. Chin J Biotechnol, 2021, 37(12): 4243-4253. | |
[25] | Gallardo-Fernández M, Valls-Fonayet J, Valero E, et al. Isotopic labelling-based analysis elucidates biosynthesis pathways in Saccharomyces cerevisiae for Melatonin, Serotonin and Hydroxytyrosol formation[J]. Food Chem, 2022, 374: 131742. |
[26] | Furuya T, Kino K. Catalytic activity of the two-component flavin-dependent monooxygenase from Pseudomonas aeruginosa toward cinnamic acid derivatives[J]. Appl Microbiol Biotechnol, 2014, 98(3): 1145-1154. |
[27] | Wu L, Wen YD, Chen WY, et al. Simultaneously deleting ADH2 and THI3 genes of Saccharomyces cerevisiae for reducing the yield of acetaldehyde and fusel alcohols[J]. FEMS Microbiol Lett, 2021, 368(15): fnab094. |
[28] |
Pirkov I, Norbeck J, Gustafsson L, et al. A complete inventory of all enzymes in the eukaryotic methionine salvage pathway[J]. FEBS J, 2008, 275(16): 4111-4120.
doi: 10.1111/j.1742-4658.2008.06552.x pmid: 18625006 |
[29] | Hazelwood LA, Daran JM, van Maris AJA, et al. The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism[J]. Appl Environ Microbiol, 2008, 74(8): 2259-2266. |
[30] | Averesch NJH, Prima A, Krömer JO. Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae[J]. Bioprocess Biosyst Eng, 2017, 40(8): 1283-1289. |
[31] |
Hazelwood LA, Tai SL, Boer VM, et al. A new physiological role for Pdr12p in Saccharomyces cerevisiae: export of aromatic and branched-chain organic acids produced in amino acid catabolism[J]. FEMS Yeast Res, 2006, 6(6): 937-945.
pmid: 16911515 |
[32] |
Reifenrath M, Boles E. Engineering of hydroxymandelate synthases and the aromatic amino acid pathway enables de novo biosynthesis of mandelic and 4-hydroxymandelic acid with Saccharomyces cerevisiae[J]. Metab Eng, 2018, 45: 246-254.
doi: S1096-7176(17)30407-X pmid: 29330068 |
[33] | Ishchuk OP, Domenzain I, Sánchez BJ, et al. Genome-scale modeling drives 70-fold improvement of intracellular heme production in Saccharomyces cerevisiae[J]. Proc Natl Acad Sci USA, 2022, 119(30): e2108245119. |
[1] | SHEN Zhen-hui, CAO Yao, YANG Lin-lei, LUO Xiang-ying, ZI Ling-shan, LU Qing-qing, LI Rong-chun. Cloning and Bioinformatics Analysis of the Ergothioneine Biosynthesis Genes in Naematelia aurantialba and Stereum hirsutum [J]. Biotechnology Bulletin, 2024, 40(7): 259-272. |
[2] | HU Jin-jin, LI Su-zhen, MA Xu-hui, LIU Xiao-qing, XIE Shan-shan, JIANG Hai-yang, CHEN Ru-mei. Regulation of Maize Anthocyanin Biosynthesis Metabolism [J]. Biotechnology Bulletin, 2024, 40(6): 34-44. |
[3] | YU Xin-lei, HE Jie-wang, LIN Guo-ping, LI Jin-hai, WANG Da-ai, YUAN Yue-bin, LIU Sheng-gao, LI Zhi-hao, TAO De-xin. Metabolome Difference Analysis of Fermented Cigar Tobacco Leaves in Summer and Winter [J]. Biotechnology Bulletin, 2024, 40(6): 260-270. |
[4] | YANG Yan, HU Yang, LIU Ni-ru, YIN Lu, YANG Rui, WANG Peng-fei, MU Xiao-peng, ZHANG Shuai, CHENG Chun-zhen, ZHANG Jian-cheng. Cloning and Functional Analysis of MbbZIP43 Gene in ‘Hongmantang’ Red-flesh Apple [J]. Biotechnology Bulletin, 2024, 40(2): 146-159. |
[5] | GUAN Zhi-jing, SUN Chao. Research Progress in the Compartmentalization of Plant Specialized Metabolism [J]. Biotechnology Bulletin, 2024, 40(1): 1-11. |
[6] | WANG Jun-fang, HUANG Qiu-bin, ZHANG Piao-dan, ZHANG Peng-pai. Structure and Biosynthesis of Surfactin as well as Its Role in Biological Control [J]. Biotechnology Bulletin, 2024, 40(1): 100-112. |
[7] | ZHANG Jin-wei, WU Yuan-xia, SUN Jing, LI Xiao-kai, LU Lu, LI Zhou-quan, GE Liang-peng. Effects of Commensal Microbiota on Intestinal Development, Metabolism, and Mitochondrial Function in Piglets [J]. Biotechnology Bulletin, 2024, 40(1): 332-343. |
[8] | CHEN Zhi-min, LI Cui, WEI Ji-tian, LI Xin-ran, LIU Yi, GUO Qiang. Research Progress in the Regulation of Chlorogenic Acid Biosynthesis and Its Application [J]. Biotechnology Bulletin, 2024, 40(1): 57-71. |
[9] | LI Liang, XU Shan-shan, JIANG Yan-jun. Research Progress in the Production of Ergothioneine by Biosynthesis [J]. Biotechnology Bulletin, 2024, 40(1): 86-99. |
[10] | XU Fa-di, XU Kang, SUN Dong-ming, LI Meng-lei, ZHAO Jian-zhi, BAO Xiao-ming. Research Progress in Second-generation Fuel Ethanol Technology Based on Poplar(Populus sp.) [J]. Biotechnology Bulletin, 2023, 39(9): 27-39. |
[11] | YE Yun-fang, TIAN Qing-yin, SHI Ting-ting, WANG Liang, YUE Yuan-zheng, YANG Xiu-lian, WANG Liang-gui. Research Progress in the Biosynthesis and Regulation of β-ionone in Plants [J]. Biotechnology Bulletin, 2023, 39(8): 91-105. |
[12] | WANG Ling, ZHUO Shen, FU Xue-sen, LIU Zi-xuan, LIU Xiao-rong, WANG Zhi-hui, ZHOU Ri-bao, LIU Xiang-dan. Advances in the Biosynthetic Pathways and Related Genes of Lotus Alkaloids [J]. Biotechnology Bulletin, 2023, 39(7): 56-66. |
[13] | CHENG Ting, YUAN Shuai, ZHANG Xiao-yuan, LIN Liang-cai, LI Xin, ZHANG Cui-ying. Research Progress in the Regulation of Isobutanol Synthesis Pathway in Saccharomyces cerevisiae [J]. Biotechnology Bulletin, 2023, 39(7): 80-90. |
[14] | JIANG Qing-chun, DU Jie, WANG Jia-cheng, YU Zhi-he, WANG Yun, LIU Zhong-yu. Expression and Function Analysis of Transcription Factor PcMYB2 from Polygonum cuspidatum [J]. Biotechnology Bulletin, 2023, 39(5): 217-223. |
[15] | ZHENG Huan, LIN Dong-mei, LIU Jun-yuan, ZHANG Yin-lian, LIN Biao-sheng, LIN Zhan-xi, LI Jing. Analysis of Amino Acid Metabolism Difference Between Fruiting Body and Mycelium of Taiwanofungus camphoratus by LC-QTOF-MS Metabonomics [J]. Biotechnology Bulletin, 2023, 39(5): 254-266. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||