Research Progress in Metabolic Engineering for Modification and Syngas-directed Conversion by Acetogenic Clostridium

doi:10.13560/j.cnki.biotech.bull.1985.2025-0283

Abstract

Abstract:

The development of microbial carbon sequestration technology is crucial for curbing global warming. Acetogenic Clostridium can fix one-carbon gases, such as CO₂ and CO, and convert them into chemical products, such as ethanol, thereby reducing dependence on fossil fuels, which makes acetogenic Clostridium a promising strain for converting syngas into high-value products on a large scale. This review summarizes the research progress in metabolic engineering modifications of acetogenic Clostridium and the directional conversion of syngas. Firstly, the energy metabolism modes of acetogenic Clostridium have been analyzed, and the energy conservation mechanisms of acetogenic Clostridium have been elaborated. Then, various tools for genetic manipulation of acetogenic Clostridium have been compared and summarized. Finally, the current prospects and challenges in the two major directions of metabolic engineering modification of acetogenic Clostridium have been sorted out. In conclusion, this paper summarizes the research progress in metabolic engineering ofacetogenic Clostridium inrecent years, aiming to provide references for more comprehensive understanding of its syngas conversion potential, and promote the development of renewable energy production platform using industrial acetogenic Clostridium.

Key words: acetogenic Clostridium, energy conservation mechanism, metabolic engineering, syngas

LIU Xiu-ping, ZHU Xing-yu, WANG Guang-yi. Research Progress in Metabolic Engineering for Modification and Syngas-directed Conversion by Acetogenic Clostridium[J]. Biotechnology Bulletin, doi: 10.13560/j.cnki.biotech.bull.1985.2025-0283.

Figures/Tables 3

Fig. 1 Wood-Ljungdahl pathwayFdh: Formate dehydrogenase; Fhs: formyl-THF synthetase; FTC: formyl-THF cyclohydrolase; MTDH: methylene-THF dehydrogenase; MetF: methylene-THF reductase; MetR: methyltransferase; CODH/ACS: carbon monoxide dehydrogenase/acetyl-CoA synthase; Ald: acetaldehyde dehydrogenase; AdhE: acetaldehyde/alcohol dehydrogenase; Pta: phosphotransacetylase; Ack: acetate kinase; Aor: aldehyde: ferredoxin oxidoreductase

Fig. 2 Energy metabolism mechanism in Acetogenic Clostridium

Fig. 3 Metabolic pathways of synthetic products in Acetogenic ClostridiumThlA/Thl2: Thiolase; Hbd/Hbd2: 3-hydroxybutyryl-CoA dehydrogenase; Crt/Crt2: crotonase; Bcd/Bcd2: butyryl-CoA dehydrogenase; AdhE: acetaldehyde/alcohol dehydrogenase; ATF1: alcohol Acetyltransferase; Ptb/Ptf2: Phosphotransferase; Buk: butyrate kinase; SAAT: strawberry Alcohol Acyltransferase; ETH1: ethanol hexanoyltransferase I; MvaS/E: hydroxymethylglutaryl-CoA synthase/reductase; Idi: isopentenyl pyrophosphate isomerase; IspS: isoprene synthase; CtfAB: acetoacetyl-CoA:acetate/butyrate-CoA transferase; Adc: acetoacetate decarboxylase; Sadh: secondary alcohol dehydrogenase; bdhA: 3-hydroxybutyrate dehydrogenase; Pta: Phosphotransacetylase; Ack: Acetate kinase; Ald: Acetaldehyde dehydrogenase; Aor: aldehyde: ferredoxin oxidoreductase; Etf: electron transfer flavoprotein; Fak: fatty acid kinase; AldH: aldehyde dehydrogenasel; Adh: alcohol dehydrogenase; PFOR: pyruvate: ferredoxin oxidoreductase; AlsS: acetolactate synthase; AldC: acetoin decarboxylase; 2,3-Bdh: 2,3-butanediol dehydrogenase; Ldh: lactate dehydrogenase; PhaC: Polyhydroxyalkanoate Synthase

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