Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat

doi:10.13560/j.cnki.biotech.bull.1985.2024-0718

Abstract

Abstract:

Objective The objective of this study is to perform the genome-wide identification of the wheat PDIL (Protein Disulfide Isomerase) gene family members, and to analyze their expression patterns in different organs and developmental stages of wheat, as well as in response to biotic and abiotic stress, which lays the foundation for clarifying the function of wheat PDIL gene family. Method Using protein sequences of PDIL genefamily of Arabidopsis thaliana and Gramineae crop barley as bait, genome-wide identification of wheat PDIL family genes was conducted in the genome database. Then the physicochemical properties, phylogenetics, chromosome position and gene structure were analyzed using bioinformatics methods. And the expression pattern of PDILs genes in wheat growth and development, biotic and abiotic adversity were analyzed. Result The 31 wheat PDIL gene family members were identified as hydrophilic proteins, of which 26 were stable proteins. Subcellular localization prediction indicated that wheat PDIL proteins were mainly distributed in the cytoplasm and chloroplasts, as well as in the endoplasmic reticulum and nucleus. The wheat PDIL gene family could be divided into 7 evolutionary branches, and the gene structure and conserved motif of the same branch were similar or identical, and they were not uniformly distributed on 19 chromosomes. The promoters of wheat PDIL gene family members contained multiple cis-acting elements that responded to stress and associated with five types of plant hormones, including salicylic acid (SA), methyl jasmonate (MeJA), gibberellin (GA), abscisic acid (ABA), and auxin (IAA), and a few genes also contained elements related to circadian rhythm control and seed trait regulation. Transcriptome data showed that TaPDIL4-1A, TaPDIL4-1B, and TaPDIL4-1D genes had high expressions in tissues at different growth stages. Transcriptome analysis and RT-PCR results showed that the relative expression of TaPDIL4-1B increased after infection with Fusarium graminearum, and the expression of TaPDIL1-4A gene increased under drought stress. Conclusion The 31 members of the wheat PDIL gene family have conservation during evolution, not only participating in wheat quality formation, but also widely involved in biotic and abiotic stress regulation. Among them, the TaPDIL4-1B gene might be involved in the regulation of wheat FHB resistance, while the TaPDIL1-4A gene might be involved in the response to wheat drought stress.

Key words: wheat, PDIL gene family, expression analysis, biotic stress, abiotic stress

GE Shi-jie, LIU Yi-de, ZHANG Hua-dong, NING Qiang, ZHU Zhan-wang, WANG Shu-ping, LIU Yi-ke. Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat[J]. Biotechnology Bulletin, 2025, 41(2): 85-96.

Figures/Tables 9

References 34

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引物名称 Primer name	序列 Sequence （5′-3′）
Actin-F	AGTGGAGGTTCTACCATGTTTCCT
Actin-R	CACTGTATTTCCTTTCAGGTGGTG
TaPDIL1-4A-F	GAGCCTGTGAAGGTAGTTGTGG
TaPDIL1-4A-F	GTGACGAAGTAGAGGGTGGG
TaPDIL2-3B-F	GAGACCACGGTTCTTGCTTACA
TaPDIL2-3B-F	CGATGCCACGGAGATACTTGC
TaPDIL3-7D-F	CCGCTCAGAACCAGTACCAC
TaPDIL3-7D-F	TTCACCGAAGCATCAATACG
TaPDIL4-1B-F	CTGTCAAGCACGGAAAGATC
TaPDIL4-1B-F	AGAGGAGAAGGCTGAAAGAAT
TaPDIL5-5A-F	TTTGGTATTTGGTGCCGATAA
TaPDIL5-5A-F	TTTCTTCCTTCTGCCTTTGAGT
TaPDIL7-6B-F	GTATGAAGTGGTTGAGGGTTTAG
TaPDIL7-6B-F	GGAGCGTCTGCCCAATGTCT

引物名称 Primer name	序列 Sequence （5′-3′）
Actin-F	AGTGGAGGTTCTACCATGTTTCCT
Actin-R	CACTGTATTTCCTTTCAGGTGGTG
TaPDIL1-4A-F	GAGCCTGTGAAGGTAGTTGTGG
TaPDIL1-4A-F	GTGACGAAGTAGAGGGTGGG
TaPDIL2-3B-F	GAGACCACGGTTCTTGCTTACA
TaPDIL2-3B-F	CGATGCCACGGAGATACTTGC
TaPDIL3-7D-F	CCGCTCAGAACCAGTACCAC
TaPDIL3-7D-F	TTCACCGAAGCATCAATACG
TaPDIL4-1B-F	CTGTCAAGCACGGAAAGATC
TaPDIL4-1B-F	AGAGGAGAAGGCTGAAAGAAT
TaPDIL5-5A-F	TTTGGTATTTGGTGCCGATAA
TaPDIL5-5A-F	TTTCTTCCTTCTGCCTTTGAGT
TaPDIL7-6B-F	GTATGAAGTGGTTGAGGGTTTAG
TaPDIL7-6B-F	GGAGCGTCTGCCCAATGTCT

序列号 Sequence ID	基因 Gene	氨基酸数量 Number of amino acids	相对分子量 Relative molecular weight/Da	理论等电点Theoretical pI	不稳定系数Instability index	脂溶指数Aliphatic index	GRAVY	亚细胞定位 Subcellular localization
TraesCS2A02G306500.1	TaPDIL1-2A	517	57 040.81	4.64	37.21	90.87	-0.082	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G323200.1	TaPDIL1-2B	518	57 125.01	4.65	37.84	89.96	-0.083	叶绿体、细胞质Chloroplast， cytoplasm
TraesCS2D02G305000.1	TaPDIL1-2D	517	56 952.69	4.71	35.29	90.14	-0.095	叶绿体、细胞质、核 Chloroplast， cytoplasm， and nucleus
TraesCS4A02G214200.2	TaPDIL1-4A	515	56 579.13	4.99	34.70	85.15	-0.24	内质网Endoplasmic reticulum
TraesCS4B02G101800.3	TaPDIL1-4B	512	56 441.08	5.03	36.30	83.91	-0.249	内质网Endoplasmic reticulum
TraesCS4D02G098400.1	TaPDIL1-4D	515	56 629.18	4.96	33.86	83.44	-0.246	内质网Endoplasmic reticulum
TraesCS3B02G532200.1	TaPDIL2-3B	582	63 587.51	4.61	33.75	89.13	-0.208	叶绿体、核Chloroplast， nucleus
TraesCS6A02G000600.1	TaPDIL2-6A	402	43 760.01	4.58	34.06	88.03	-0.21	叶绿体Chloroplast
TraesCS6B02G005500.1	TaPDIL2-6B	588	63 851.97	4.74	35.34	88.77	-0.172	叶绿体Chloroplast
TraesCS6D02G004700.1	TaPDIL2-6D	588	64 389.68	4.68	33.80	86.66	-0.222	叶绿体Chloroplast
TraesCS6A02G001100.1	TaPDIL2.1-6A	579	63 175.13	4.57	34.68	88.06	-0.21	叶绿体、核Chloroplast， and nucleus
TraesCS6B02G005100.1	TaPDIL2.1-6B	594	64 692.05	4.80	47.11	84.55	-0.349	叶绿体Chloroplast
TraesCS6D02G005200.1	TaPDIL2.1-6D	587	63 940.12	4.59	35.28	88.03	-0.198	叶绿体Chloroplast
TraesCS7A02G123900.1	TaPDIL3-7A	541	59 594.01	4.95	36.86	94.01	-0.157	叶绿体Chloroplast
TraesCS7B02G022800.1	TaPDIL3-7B	555	60 860.45	4.96	33.39	94.99	-0.146	叶绿体Chloroplast
TraesCS7D02G121800.1	TaPDIL3-7D	544	59 621.97	5.03	31.02	92.96	-0.147	叶绿体Chloroplast
TraesCS1A02G097700.1	TaPDIL4-1A	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1B02G126100.1	TaPDIL4-1B	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1D02G106500.1	TaPDIL4-1D	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS5A02G234400.1	TaPDIL5-5A	440	47 183.9	5.24	36.60	86.32	-0.145	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5B02G232900.1	TaPDIL5-5B	440	47 221.96	5.36	37.28	86.09	-0.152	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5D02G241200.1	TaPDIL5-5D	440	47 174.9	5.30	37.99	86.55	-0.135	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2A02G220100.1	TaPDIL6-2A	415	46 112.8	6.90	40.05	87.55	-0.186	叶绿体Chloroplast
TraesCS2B02G245600.1	TaPDIL6-2B	485	54 407.99	6.90	39.56	87.34	-0.192	叶绿体Chloroplast
TraesCS2D02G225800.1	TaPDIL6-2D	485	54 405.03	6.90	39.71	88.35	-0.172	叶绿体Chloroplast
TraesCS2A02G304300.1	TaPDIL7-2A	413	46 293.1	4.89	39.70	92.28	-0.051	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G321000.1	TaPDIL7-2B	417	46 621.5	4.89	41.42	93.05	-0.047	叶绿体、细胞核 Chloroplast， and nucleus
TraesCS2D02G302900.1	TaPDIL7-2D	451	50 773.16	5.22	46.40	86.03	-0.158	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS6A02G220100.1	TaPDIL7-6A	485	54 391.99	5.17	37.12	94.17	-0.046	叶绿体Chloroplast
TraesCS6B02G261500.2	TaPDIL7-6B	418	46 287.77	4.85	42.74	92.58	-0.077	叶绿体Chloroplast
TraesCS6D02G215100.1	TaPDIL7-6D	417	46 458.32	5.24	37.98	92.78	-0.072	叶绿体Chloroplast

序列号 Sequence ID	基因 Gene	氨基酸数量 Number of amino acids	相对分子量 Relative molecular weight/Da	理论等电点Theoretical pI	不稳定系数Instability index	脂溶指数Aliphatic index	GRAVY	亚细胞定位 Subcellular localization
TraesCS2A02G306500.1	TaPDIL1-2A	517	57 040.81	4.64	37.21	90.87	-0.082	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G323200.1	TaPDIL1-2B	518	57 125.01	4.65	37.84	89.96	-0.083	叶绿体、细胞质Chloroplast， cytoplasm
TraesCS2D02G305000.1	TaPDIL1-2D	517	56 952.69	4.71	35.29	90.14	-0.095	叶绿体、细胞质、核 Chloroplast， cytoplasm， and nucleus
TraesCS4A02G214200.2	TaPDIL1-4A	515	56 579.13	4.99	34.70	85.15	-0.24	内质网Endoplasmic reticulum
TraesCS4B02G101800.3	TaPDIL1-4B	512	56 441.08	5.03	36.30	83.91	-0.249	内质网Endoplasmic reticulum
TraesCS4D02G098400.1	TaPDIL1-4D	515	56 629.18	4.96	33.86	83.44	-0.246	内质网Endoplasmic reticulum
TraesCS3B02G532200.1	TaPDIL2-3B	582	63 587.51	4.61	33.75	89.13	-0.208	叶绿体、核Chloroplast， nucleus
TraesCS6A02G000600.1	TaPDIL2-6A	402	43 760.01	4.58	34.06	88.03	-0.21	叶绿体Chloroplast
TraesCS6B02G005500.1	TaPDIL2-6B	588	63 851.97	4.74	35.34	88.77	-0.172	叶绿体Chloroplast
TraesCS6D02G004700.1	TaPDIL2-6D	588	64 389.68	4.68	33.80	86.66	-0.222	叶绿体Chloroplast
TraesCS6A02G001100.1	TaPDIL2.1-6A	579	63 175.13	4.57	34.68	88.06	-0.21	叶绿体、核Chloroplast， and nucleus
TraesCS6B02G005100.1	TaPDIL2.1-6B	594	64 692.05	4.80	47.11	84.55	-0.349	叶绿体Chloroplast
TraesCS6D02G005200.1	TaPDIL2.1-6D	587	63 940.12	4.59	35.28	88.03	-0.198	叶绿体Chloroplast
TraesCS7A02G123900.1	TaPDIL3-7A	541	59 594.01	4.95	36.86	94.01	-0.157	叶绿体Chloroplast
TraesCS7B02G022800.1	TaPDIL3-7B	555	60 860.45	4.96	33.39	94.99	-0.146	叶绿体Chloroplast
TraesCS7D02G121800.1	TaPDIL3-7D	544	59 621.97	5.03	31.02	92.96	-0.147	叶绿体Chloroplast
TraesCS1A02G097700.1	TaPDIL4-1A	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1B02G126100.1	TaPDIL4-1B	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS1D02G106500.1	TaPDIL4-1D	367	40 260.95	6.17	33.38	85.10	-0.287	内质网Endoplasmic reticulum
TraesCS5A02G234400.1	TaPDIL5-5A	440	47 183.9	5.24	36.60	86.32	-0.145	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5B02G232900.1	TaPDIL5-5B	440	47 221.96	5.36	37.28	86.09	-0.152	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS5D02G241200.1	TaPDIL5-5D	440	47 174.9	5.30	37.99	86.55	-0.135	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2A02G220100.1	TaPDIL6-2A	415	46 112.8	6.90	40.05	87.55	-0.186	叶绿体Chloroplast
TraesCS2B02G245600.1	TaPDIL6-2B	485	54 407.99	6.90	39.56	87.34	-0.192	叶绿体Chloroplast
TraesCS2D02G225800.1	TaPDIL6-2D	485	54 405.03	6.90	39.71	88.35	-0.172	叶绿体Chloroplast
TraesCS2A02G304300.1	TaPDIL7-2A	413	46 293.1	4.89	39.70	92.28	-0.051	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS2B02G321000.1	TaPDIL7-2B	417	46 621.5	4.89	41.42	93.05	-0.047	叶绿体、细胞核 Chloroplast， and nucleus
TraesCS2D02G302900.1	TaPDIL7-2D	451	50 773.16	5.22	46.40	86.03	-0.158	叶绿体、细胞质 Chloroplast， and cytoplasm
TraesCS6A02G220100.1	TaPDIL7-6A	485	54 391.99	5.17	37.12	94.17	-0.046	叶绿体Chloroplast
TraesCS6B02G261500.2	TaPDIL7-6B	418	46 287.77	4.85	42.74	92.58	-0.077	叶绿体Chloroplast
TraesCS6D02G215100.1	TaPDIL7-6D	417	46 458.32	5.24	37.98	92.78	-0.072	叶绿体Chloroplast

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