Three New Chinese Records of Agaricales Species from Shanxi Province， China

doi:10.13560/j.cnki.biotech.bull.1985.2026-0344

Abstract

Abstract:

Objective To accurately identify three agaric specimens collected from the Lyuliang and Taihang Mountains in Shanxi Province that are suspected to be new records for China, and to enrich the macrofungal species diversity of Shanxi Province. Method A combination of morphological and molecular systematics was employed to determine the taxonomic status of the three species. Based on observations of macro-morphology and microstructural characteristics, and using ITS and other multilocus sequences, maximum likelihood (ML) and Bayesian inference (BI) phylogenetic trees were constructed for comprehensive analysis. Result Based on comprehensive morphological and molecular data, the three species were identified as Entoloma zuccherellii, Inocybe rufobrunnea, and Staktophyllus guttulatus, which are newly recorded species in China. The phylogenetic analysis results were highly consistent with the morphological identification. Conclusion This study reports the distribution of the three agaric species in China for the first time, expanding their known geographic range and providing new material for biogeographic and evolutionary research on related taxa.

Key words: Agaricales, phylogenetic analysis, morphological analysis, new records

ZHANG Qian-da, MA Xiao-qing, LIU Jin-tao, CHANG Ming-chang, MENG Jun-long, WANG Shu-rong. Three New Chinese Records of Agaricales Species from Shanxi Province， China[J]. Biotechnology Bulletin, 2026, 42(10): 250-256.

Figures/Tables 6

Fig. 1 Basidiomata and microstructure of Entoloma zuccherelliiA-B: Basidiomata. C-H: Spores. I-K: Basidia. L: Pleurocystidia. Bars: A-B=1 cm; C-H=5 μm; I-J=10 μm

Fig. 2 E. zuccherellii ML phylogenetic tree based on ITS, nLSU, mtSSU和RPB2 sequencesThe statistical values of the internal nodes are the values obtained by ML/BI. The sequences obtained in this study are represented in bold font. Leepista ovispora is selected as the outgroup, and the scale bar represents the evolutionary distance

Fig. 3 Basidiomata and microstructure of Inocybe rufobrunneaA-B: Basidiomata. C-H: Spores. I-J: Basidia. K-L: Pleurocystidia. M-N: Cheilocistidia. O-P: Caulocistidia. Bars: A-B=0.5 cm, C-H=5 μm, I-P=20 μm

Fig. 4 I. rufobrunnea ML phylogenetic tree based on ITS and nLSU sequencesThe statistical values of the internal nodes are the values obtained by ML/BI. The sequences obtained in this study are represented in bold font. Pseudosperma spurium is selected as the outer taxa, and the ruler represents the evolutionary distance

Fig. 5 Basidiomata and microstructure of Staktophyllus guttulatusA-B: Basidiomata. C-H: Spores. I-K: Basidia. L-N: Cheilocystidia. O-Q: Caulocystidia. R-T: Pileocystidia. Bars: A-B=1 cm, C-H=5 μm, I-T=10 μm

Fig. 6 S. guttulatus ML phylogenetic tree based on ITS and nLSU sequencesThe statistical values of the internal nodes are the values obtained by ML/BI. The sequences obtained in this study are represented in bold font. Conocyybe cylindracea is selected as the outer taxa, and the ruler represents the evolutionary distance

References 30

[1]	Noordeloos M, Anton H. Tre nuove Entolomataceae (Agaricales) dall’ Italia [J]. Bolletino Del Gruppo Micologico G. Bresadola, 2000, 43(3): 23-33.
[2]	Co-David D, Langeveld D, Noordeloos ME. Molecular phylogeny and spore evolution of Entolomataceae [J]. Persoonia, 2009, 23(1): 147-176.
[3]	Morozova O, Pham THG. New species of Entoloma subgenera cubospora and Leptonia (Agaricales, Basidiomycota) from central Vietnam [J]. JoF, 2023, 9(6): 621.
[4]	Holec J, Noordeloos ME. On the infraspecific variability and taxonomic position of Entoloma zuccherellii [J]. Mycotaxon, 2010, 112(1): 283-289.
[5]	Voto P. Entoloma zuccherellii from the Nordio wood (Italy) [J]. Mycological Observations, 2022, 6: 116-121.
[6]	Favre J. Les champignons supérieurs de la zone alpine du Parc National Suisse [R]. Index of Fungi, 1955, 5(33): 105-201.
[7]	Bandini D, Oertel B, Eberhardt U. More smooth-spored species of Inocybe (Agaricales, Basidiomycota): type studies and 12 new species from Europe [J]. Persoonia, 2022, 48(1): 91-149.
[8]	Zhou YL, Deng LS, Yang SD, et al. Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China [J]. Front Microbiol, 2023, 14: 1326253.
[9]	Bresadola G. Fungi Tridentini novi, vel nondum delineati, descripti, et iconibus illustrati [M]. Vienna: C. Gerold’s Sohn, 1883.
[10]	Consiglio G, Marchetti M. Contributo alla conoscenza del Genere Panaeolus sensu lato [J]. Associazione Micologica Bresadola, 2023, 66(3): 209-286.
[11]	Seidmohammadi E, Abbasi S, Asef M. The first report of Panaeolus olivaceus and Panaeolus guttulatus from Iran [J]. Taxon Biosyst, 2019, 11(1): 23-30.
[12]	Senn-Irlet B, Nyffenegger A, Brenneisen R. Panaeolus bisporus—an adventitious fungus in central Europe, rich in psilocin [J]. Mycologist, 1999, 13(4): 176-179.
[13]	孙淑婧. 山西省林业生态旅游发展潜力与路径 [J]. 山西林业, 2024, (6): 20-21.
	Sun SJ. Development potential and path of forestry eco-tourism in Shanxi Province [J]. For Shanxi, 2024, (6): 20-21.
[14]	刘虹, 郭尚, 范黎. 山西著名野生食用蘑菇红银盘: 漏斗伞属新物种 [J]. 山西大学学报: 自然科学版, 2019, 42(1): 275-280.
	Liu H, Guo S, Fan L. Infundibulicybe hongyinpansp.nov., a well-known edible fungus from Shanxi based on both nrDNA-ITS and morphological analysis [J]. J Shanxi Univ Nat Sci Ed, 2019, 42(1): 275-280.
[15]	王术荣, 刘淑琴, 孟俊龙, 等. 山西太行山地区铦囊蘑属两新种 [J]. 菌物学报, 2022, 41(12): 1921-1931.
	Wang SR, Liu SQ, Meng JL, et al. Two new species of Melanoleuca from Taihang mountains in Shanxi Province [J]. Mycosystema, 2022, 41(12): 1921-1931.
[16]	Mao N, Yang JJ, Zhang YX, et al. Species diversity of Tricholoma (Agaricales, Tricholomataceae) from Shanxi Province of northern China with the description of four new species [J]. MycoKeys, 2025, 112: 59-80.
[17]	Mao N, Zhao TY, Zhang YX, et al. Boletaceae from Shanxi Province of northern China with descriptions of ten new species [J]. Mycosphere, 2023, 14(1): 2013-2091.
[18]	张宇欣, 毛宁, 李颖, 等. 山西省蜡伞属真菌物种多样性及一新种的描述 [J]. 微生物学通报, 2024, 51(12): 5159-5176.
	Zhang YX, Mao N, Li Y, et al. Species diversity of Hygrophorus (Hygrophoraceae, Agaricales) in Shanxi Province and description of Hygrophorus brunneodiscoides sp. nov [J]. Microbiol China, 2024, 51(12): 5159-5176.
[19]	Li T, Mao N, Fu HY, et al. Two new species of Hymenogaster (Hymenogastraceae, Agaricales) from China based on morphological and molecular markers [J]. Diversity, 2024, 16(5): 303.
[20]	Wu YQ, Liu SQ, Wang TY, et al. Geastrum sanglinense, a new species from the Manghe Rhesus Monkey National Nature Reserve, China [J]. Mycoscience, 2024, 65(1): 12-18.
[21]	Li Y, Mao N, Zhang YX, et al. Catalogue of fungi in China 2.Ramaria from northern China [J]. Mycology, 2025, 16(1): 59-90.
[22]	刘淑琴, 王浩豪, 武艳群, 等. 下迪铦囊蘑(Melanoleuca dirensis)—铦囊蘑属中国新记录种 [J]. 东北林业大学学报, 2022, 50(7): 77-80.
	Liu SQ, Wang HH, Wu YQ, et al. Melanoleuca dirensis: a newly recorded species of Melanoleuca Pat.in China [J]. J Northeast For Univ, 2022, 50(7): 77-80.
[23]	刘虹, 张泽乾, 毛宁. 基于形态特征和ITS片段分析确认山西发现极地梭孢杯伞 [J]. 中国食用菌, 2023, 42(1): 15-19.
	Liu H, Zhang ZQ, Mao N. The finding of Atractosporocybe polaris in Shanxi Province based on both morphological and molecular evidences [J]. Edible Fungi China, 2023, 42(1): 15-19.
[24]	武艳群, 何轶榕, 刘淑琴, 等. 鬼伞属中国新记录种——椰枣色孢鬼伞 [J]. 东北林业大学学报, 2023, 51(8): 136-139.
	Wu YQ, He YR, Liu SQ, et al. A new recorded species of Coprinus in China: Coprinus spadiceisporus [J]. J Northeast For Univ, 2023, 51(8): 136-139.
[25]	王腾云, 王静怡, 孟俊龙, 等. 马鞍菌属3个中国新记录 [J]. 微生物学通报, 2023, 50(8): 3372-3381.
	Wang TY, Wang JY, Meng JL, et al. Three new records of Helvella in China [J]. Microbiol China, 2023, 50(8): 3372-3381.
[26]	朱力扬, 图力古尔. 中国刺毛鬼伞属的物种多样性 [J]. 菌物学报, 2024, 43(3): 73-103.
	Zhu LY, Tuliguer. Species diversity of Tulosesus (Psathyrellaceae, agaricales) in China [J]. Mycosystema, 2024, 43(3): 73-103.
[27]	赵杰. ITS序列分析及其在植物真菌病害分子检测中的应用 [J]. 陕西农业科学, 2004, 50(4): 35-37.
	Zhao J. ITS sequence analysis and its application in molecular detection of plant fungal diseases [J]. Shaanxi J Agric Sci, 2004, 50(4): 35-37.
[28]	农倩, 张雯龙, 蓝桃菊, 等. 一株抗香蕉枯萎病DSE菌株的筛选鉴定及抗病机理初探 [J]. 热带作物学报, 2017, 38(3): 559-564.
	Nong Q, Zhang WL, Lan TJ, et al. Screening and identification of dark septate endophyte strain L-14 and its mechanism of banana Fusarium wilt disease resistance [J]. Chin J Trop Crops, 2017, 38(3): 559-564.
[29]	Liu YJ, Whelen S, Hall BD. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit [J]. Mol Biol Evol, 1999, 16(12): 1799-1808.
[30]	黄宏强, 范小燕, 陶亚群, 等. 三七黑斑病病原菌的复核鉴定 [J]. 长江大学学报: 自科版, 2016, 13(15): 6-9, 3.
	Huang HQ, Fan XY, Tao YQ, et al. Morphological and molecular identification of the Alternaria species causing black spot disease on Panax notoginseng [J]. J Yangtze Univ Nat Sci Ed, 2016, 13(15): 6-9, 3.