Identification of FBN Gene Family and Its Relationship with Carotenoids Metabolism in Lyciumbarbarum

doi:10.13560/j.cnki.biotech.bull.1985.2025-0340

Abstract

Abstract:

Objective Fibrillin (FBN) protein family members play important roles in plant growth and development, and carotenoid accumulation. The identification of FBN gene family in Lycium barbarum may provide theoretical basis for studying the functions of LbaFBN genes. Method The physicochemical properties, gene structure,conserved motif, collinearity relationship and cis-acting elements of FBN gene family in L. barbarum were analyzed by bioinformatics method. The expression patterns of LbaFBN genes of ‘Ningqi 1’ were investigated using transcriptome and RT-qPCR. The carotenoid content during fruit development was determined by high-performance liquid chromatography. The correlation between LbaFBN gene expression and carotenoid content was analyzed by R software. Result In total, thirteen LbaFBNs family members were identified in L. barbarum and classified into twelve subgroups, which were randomly distributed on 8 chromosomes. The LbaFBNs in each sub-group shared similar gene structure and conserved motifs. Several cis-acting elements related to plant growth and development, hormone, light and stress were found in the promoter region of LbaFBN members. Gene expression analysis showed that LbaFBNs family genes were expressed in different developmental stages. LbaFBN13 expressions increased gradually as the fruit development. Subcellular localization showed that LbaFBN13 protein was localized in the chloroplast. Correlation analysis indicated that the expressions of LbaFBN2/4/10, LbaFBN3/8 and LbaFBN13 were significantly and positively correlated with the content of violaxanthin and lutein, β-cryptoxanthin, and zeaxanthin dipalmitate, respectively. Conclusion Thirteen LbaFBNs family members are identified in the whole genome of L. barbarum, and the expression patterns of these genes in fruit development are different. LbaFBN2/3/4/8/10/13 gene may be involved in carotenoid metabolism.

Key words: Lycium barbarum L., FBN gene family, evolutionary analysis, differential expression, fruit development period, carotenoids metabolism, correlation analysis, subcellular localization

YIN Yue, QIN Xiao-ya, MI Jia, AN Wei, HE Jun, ZHANG Feng-feng. Identification of FBN Gene Family and Its Relationship with Carotenoids Metabolism in Lyciumbarbarum[J]. Biotechnology Bulletin, 2026, 42(3): 338-348.

Figures/Tables 12

References 27

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基因名称 Gene name	上游引物序列 Forward primer sequence （5′‒3′）	下游引物序列 Reverse primer sequence （5′‒3′）	用途 Purpose
LbaFBN1	TCACAAGTGCGGTTCTGCGT	AGGAGCACGTTCAACAATCAGGT	荧光定量 Quantitative real-time
LbaFBN2	TTGAAGGGGAGTGGCAAATGA	CCATTTGCTTTGACAATCTGCTC
LbaFBN3	GTTCGAAGCCCCTCTAGAATACAG	TAATCTTTTGCCCAAACACATCC
LbaFBN4	CAAGAGCGCAAAGGTTCAAATAC	GCATAGGGTGTTGGAGTAAAGCA
LbaFBN5	CTCCTGGAAGAGCTCAAGGTGAG	GAAGGATCAACCATTTTCAAAACG
LbaFBN6	GTGTGATACCCATAAAAAGCCGT	GAATAAACAGGTTTCCTTGGTTGC
LbaFBN8	CTCCAGCAAGTTTTCTGTTGAGG	CAAAGCACCCTTTAGTTGCTCA
LbaFBN9	CCGGGAACTTATCACAGCTGC	CTCCCCTTGTTACACGTGTATCAG
LbaFBN10	GAACTCAAGGTAGGGGGCCTAGA	GCGTCTTTGAAACACAAACAAGG
LbaFBN11	CCTCCTCTCAAGGTTCCAATTCC	TCCTTAACGAGAACAAAAAGCCC
LbaFBN12	TGCAATAACACCAGACCAGTTG	CTTGTCATCTCTGCCTATCCTCG
LbaFBN13	ACCGCCAAAGCCAAAGGAAC	TTGGGTTCTTGGACTCAAGCTGA
LbaActin	CAATCGGGTATTTCAAGGTCAAG	GAGCAGTGTTTCCCAGCATTG
LbaFBN13-GFP	ACGAGCTGTACAAGGGTACCATGG CTTCCATCTCTTCTCTG	GGATCCTCTAGATGAACTAGTTAA GGCTTCAAGAGGGGAC	亚细胞定位 Subcellular localization

基因名称 Gene name	上游引物序列 Forward primer sequence （5′‒3′）	下游引物序列 Reverse primer sequence （5′‒3′）	用途 Purpose
LbaFBN1	TCACAAGTGCGGTTCTGCGT	AGGAGCACGTTCAACAATCAGGT	荧光定量 Quantitative real-time
LbaFBN2	TTGAAGGGGAGTGGCAAATGA	CCATTTGCTTTGACAATCTGCTC
LbaFBN3	GTTCGAAGCCCCTCTAGAATACAG	TAATCTTTTGCCCAAACACATCC
LbaFBN4	CAAGAGCGCAAAGGTTCAAATAC	GCATAGGGTGTTGGAGTAAAGCA
LbaFBN5	CTCCTGGAAGAGCTCAAGGTGAG	GAAGGATCAACCATTTTCAAAACG
LbaFBN6	GTGTGATACCCATAAAAAGCCGT	GAATAAACAGGTTTCCTTGGTTGC
LbaFBN8	CTCCAGCAAGTTTTCTGTTGAGG	CAAAGCACCCTTTAGTTGCTCA
LbaFBN9	CCGGGAACTTATCACAGCTGC	CTCCCCTTGTTACACGTGTATCAG
LbaFBN10	GAACTCAAGGTAGGGGGCCTAGA	GCGTCTTTGAAACACAAACAAGG
LbaFBN11	CCTCCTCTCAAGGTTCCAATTCC	TCCTTAACGAGAACAAAAAGCCC
LbaFBN12	TGCAATAACACCAGACCAGTTG	CTTGTCATCTCTGCCTATCCTCG
LbaFBN13	ACCGCCAAAGCCAAAGGAAC	TTGGGTTCTTGGACTCAAGCTGA
LbaActin	CAATCGGGTATTTCAAGGTCAAG	GAGCAGTGTTTCCCAGCATTG
LbaFBN13-GFP	ACGAGCTGTACAAGGGTACCATGG CTTCCATCTCTTCTCTG	GGATCCTCTAGATGAACTAGTTAA GGCTTCAAGAGGGGAC	亚细胞定位 Subcellular localization

基因名称 Gene name	基因ID Gene ID	氨基酸数量 Number of amino acids	相对分子量 Molecular weight （kD）	理论等电点 Theoretical pI	总平均疏水性指数 Grand average of hydropathicity	亚细胞定位 Subcellular location
LbaFBN1	Lba01g00762	216	24.61	7.78	-0.345	叶绿体 Chloroplast
LbaFBN2	Lba02g00336	410	45.99	9.15	-0.208	细胞胞质 Cytoplasmic
LbaFBN3	Lba04g00802	332	36.45	4.73	-0.361	叶绿体 Chloroplast
LbaFBN4	Lba04g01207	699	78.47	8.09	-0.257	细胞核 Nuclear
LbaFBN5	Lba06g00949	335	38.38	9.31	-0.289	叶绿体 Chloroplast
LbaFBN6	Lba06g02827	246	27.63	9.82	-0.299	叶绿体 Chloroplast
LbaFBN7	Lba08g00868	198	22.34	7.54	-0.215	细胞核 Nuclear
LbaFBN8	Lba08g01931	302	33.68	6.78	-0.088	叶绿体 Chloroplast
LbaFBN9	Lba09g00405	279	30.17	6.61	-0.247	叶绿体 Chloroplast
LbaFBN10	Lba09g02050	289	32.20	9.04	-0.271	质膜 Plasma membrane
LbaFBN11	Lba09g02224	378	41.83	4.54	-0.352	叶绿体 Chloroplast
LbaFBN12	Lba10g00821	752	84.03	8.33	0.002	质膜 Plasma membrane
LbaFBN13	Lba12g00894	321	35.37	5.28	-0.342	叶绿体 Chloroplast

基因名称 Gene name	基因ID Gene ID	氨基酸数量 Number of amino acids	相对分子量 Molecular weight （kD）	理论等电点 Theoretical pI	总平均疏水性指数 Grand average of hydropathicity	亚细胞定位 Subcellular location
LbaFBN1	Lba01g00762	216	24.61	7.78	-0.345	叶绿体 Chloroplast
LbaFBN2	Lba02g00336	410	45.99	9.15	-0.208	细胞胞质 Cytoplasmic
LbaFBN3	Lba04g00802	332	36.45	4.73	-0.361	叶绿体 Chloroplast
LbaFBN4	Lba04g01207	699	78.47	8.09	-0.257	细胞核 Nuclear
LbaFBN5	Lba06g00949	335	38.38	9.31	-0.289	叶绿体 Chloroplast
LbaFBN6	Lba06g02827	246	27.63	9.82	-0.299	叶绿体 Chloroplast
LbaFBN7	Lba08g00868	198	22.34	7.54	-0.215	细胞核 Nuclear
LbaFBN8	Lba08g01931	302	33.68	6.78	-0.088	叶绿体 Chloroplast
LbaFBN9	Lba09g00405	279	30.17	6.61	-0.247	叶绿体 Chloroplast
LbaFBN10	Lba09g02050	289	32.20	9.04	-0.271	质膜 Plasma membrane
LbaFBN11	Lba09g02224	378	41.83	4.54	-0.352	叶绿体 Chloroplast
LbaFBN12	Lba10g00821	752	84.03	8.33	0.002	质膜 Plasma membrane
LbaFBN13	Lba12g00894	321	35.37	5.28	-0.342	叶绿体 Chloroplast