Analysis of Response of Lycoris radiata Leaves to Heat Stress Revealed by Untargeted Metabolomics

doi:10.13560/j.cnki.biotech.bull.1985.2025-0874

Abstract

Abstract:

Objective To decipher the metabolic response characteristics and associated pathways in Lycoris leaves under heat stress, and to provide a theoretical basis for cultivating new Lycoris germplasm that can tolerate extreme high-temperature habitats. Method Using Lycoris radiata as plant material, heat stress treatment was conducted at 42 ℃ in a controlled environment chamber. Leaf samples were collected at 0 h (control), 6 h, and 12 h after treatment initiation, followed by untargeted metabolomics profiling via LC-MS technology. Result Across three comparison groups (HS_6 h vs HS_0 h, HS_12 h vs HS_0 h, and HS_12 h vs HS_6 h), 338, 426, and 262 differential metabolites were identified, respectively. During early heat stress (42 ℃, 6 h), metabolic activity was significantly suppressed overall. Conversely, prolonged exposure (42 ℃, 12 h) triggered a metabolic reprogramming shift dominated by up-regulation. Further analysis revealed that lipids, amino acids and derivatives constituted the major classes of differential metabolites under heat stress. Notably, lipids were mainly down-regulated during the initial phase of heat stress, whereas amino acids and derivatives showed significant up-regulation and accumulation in the later stress stage. KEGG pathway enrichment analysis revealed that heat stress-induced differential metabolites were primarily enriched in: Lipid metabolic pathways driven by phosphatidylcholine hydrolysis (encompassing four sub-pathways: Linoleic acid metabolism, arachidonic acid metabolism, alpha-linolenic acid metabolism, and glycerophospholipid metabolism), amino acid metabolic pathways characterized by significant accumulation of multiple amino acids (e.g., L-proline, L-phenylalanine) (involving three core sub-pathways: Aminoacyl-tRNA biosynthesis, D-amino acid metabolism and phenylalanine metabolism) and biosynthesis of secondary metabolites. Conclusion L. radiata employs a synergistic strategy combining “lipid-mediated sustained regulation of membrane homeostasis” with “reinforcement of amino acid-driven defense networks in later stages” to collectively establish its thermal adaptation network.

Key words: Lycoris radiata, heat stress, metabolomics, lipid metabolism, amino acid metabolism

YOU Xin, WANG Xi, ZHANG Xin-yu, WEI Xu-ying, CHENG Hua, CAI Jun-huo. Analysis of Response of Lycoris radiata Leaves to Heat Stress Revealed by Untargeted Metabolomics[J]. Biotechnology Bulletin, 2026, 42(7): 173-181.

Figures/Tables 7

Fig. 1 All classification of metabolites identified in the leaves of Lycoris radiata

Fig. 2 Principal component (PC) analysis

Fig. 3 OPLS-DA score diagram

Fig. 4 Volcano diagram of differential metabolites in the leaves of L. radiata under heat stress

Fig. 5 Categories of differential metabolites (A) and analysis of the top 5 differential metabolites by fold change (B) in the leaves of L. radiata under heat stress

Fig. 6 KEGG enrichment bubble diagram of differential metabolites in the leaves of L. radiata under heat stress

Fig. 7 Pathway diagram of lipid metabolism (A) and amino acid metabolism (B) in the leaves of L. radiata under heat stress

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