Abstract:

Introducing other microorganisms generally can induce microorganisms to regulate their metabolism which is based on interspecies interactions. This research screened a strain of fungus named as Aspergillus fumigatus M03 that can induce Sorangium cellulosum to overproduce epthilones. The yields of epothilone A and B can be enhanced 72.3% and 59.7% respectively by the organic crude extracts from A.
fumigatus M03. Appropriate amount of its intracellular aqueous crude extracts also can improve the yields of epothilone A and B each by 25.6% and 24.7%, whereas the capability of improving disappears when the crude extracts are dealt with high-temperature, and inhibition of epothilones biosynthesis will show when the concentration of the crude extracts is high in fermentation broth of Sorangium cellulosum. The fermentation broth of A. fumigatus M03 does not promote biosyntheses of epothilones, but tends to inhibit them as the concentration increases.

Key words: Interspecies interactions, Induction, Metabolic regulation, Aspergillus fumigatus, Sorangium cellulosum, Epothilones