Identification of the Mycoparasites from Aecidium pourthiaea and Screening

doi:10.13560/j.cnki.biotech.bull.1985.2019-1154

Abstract

Abstract: Three Pestalotiopsis strains PG52,PG53 and PG90,which are mycoparasites of Aecidium pourthiaea,will be identified to species,and the optimal toxigenic medium of the 3 mycoparasites are screened in this study,for laying a foundation for the isolation and identification of toxins. The phylogenetic trees of 3 strains were constructed by PAUP software,and combined with morphological characteristics,they were identified to species. The crude extracts prepared from different medium were used to treat the aeciospores of A. pourthiaea and Cronartium ribicola,and the optimal toxigenic medium were screened by Trypan blue staining and bioassay. The results of molecular identification showed that PG52 and PG90 belonged to the same branch,and they were in the same big branch with Pestalotiopsis kenyana and Pestalotiopsis oryza. PG53 and Pestalotiopsis telopeae belonged to the same branch. On the morphological characteristics,the morphological description of PG53 was basically consistent with that of P. telopeae. The morphological characteristics of PG52 and PG90 were consistent with the morphological description of P. kenyana. The effects of treating spores by the crude extracts from PG52 in the improved Fries medium were the best,and also that by the crude extracts from PG53 and PG90 in the modified M-1-D medium were the best. In conclusion,PG52 and PG90 belong to P. kenyana. PG53 belong to P. telopeae. The optimal toxigenic medium for PG52 strain is the improved Fries medium,and the optimal toxigenic medium for both PG53 and PG90 strains was the modified M-1-D medium.

Key words: mycoparasite, Pestalotiopsis, toxin, molecular identification, Aecidium pourthiaea

SUI Guo-qiang, ZHANG Deng-yun, KONG Lei, CHEN Yu-hui, LI Jing. Identification of the Mycoparasites from Aecidium pourthiaea and Screening[J]. Biotechnology Bulletin, 2020, 36(7): 104-111.

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