MicroRNA-21 Promotes the Proliferation and Migration of Esophageal Squamous Carcinoma Cell via Activating ERK1/2/MAPK Pathway

Abstract

Abstract: It was to analyse the underlying mechanism of how miR-21 promotes proliferation and invasion in ESCC. Both plate colony formation and wound-healing assays showed that proliferation and migration of ESCC cell line Eca109 were increased in miR-21 mimics group and decreased in miR-21 inhibitor group after transfection into Eca109 cells with miR-21 mimics, miR-21 inhibitor and scramble sequence, respectively. Meanwhile, Western blotting results showed p-ERK1/2 expression was elevated in miR-21 mimics group, whereas decreased in miR-21 inhibitor group. The findings demonstrated that miR-21 promotes the proliferation and migration of Eca109 cell through activating ERK1/2/MAPK pathway；miR-21 can positively regulate and control the ERK1/2/MAPK signaling pathway at post-transcriptional level in Eca109 cells.

Key words: MicroRNA-21, ERK1/2, Proliferation, Migration, Esophageal squamous cell carcinoma

Liu Fengxia, Zhang Panpan, Li Jianyong, Zheng Shutao, Lu Xiaomei, Liu Wenya. MicroRNA-21 Promotes the Proliferation and Migration of Esophageal Squamous Carcinoma Cell via Activating ERK1/2/MAPK Pathway[J]. Biotechnology Bulletin, 2013, 0(12): 189-193.

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