Isolation and Identification of a Heterotrophic Nitrifying Bacterium and  Its Mutation Breeding by UV-irradiation

Abstract

Abstract: A strain of heterotrophic nitrification bacteria named N18 was isolated from activated sludge of fermentation wastewater treatment using heterotrophic nitrification medium, and identified as Pseudomonas sp. according to the analysis of its16S rDNA gene. By UV-irradiation and 8 mutants was selected by high concentration of (NH4 )2 SO4 medium, and a mutant NF34 with highest rate of NH4 +-N removal was obtained. Studied on its function of heterotrophic nitrification, the result showed that the rate of NH4 +-N removal of strain NF34 was 11.99% higher than the original strain N18, the nitrification rate of different initial ammonia concentration 100, 200, 500 mg/L were 6.14%, 11.49%, 10.75% higher than the original strain, respectively. Little nitrite accumulated during nitrifying process. It proved that UV-mutation is an effective means to obtain nitrifying bacteria with highly nitrification activity.

Key words: Biological nitrogen removal, Heterotrophic nitrification, Pseudomonas sp. , UV-irradiation

Zheng Lixia, Zhang Dawei. Isolation and Identification of a Heterotrophic Nitrifying Bacterium and Its Mutation Breeding by UV-irradiation[J]. Biotechnology Bulletin, 2013, 0(12): 184-188.

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Isolation and Identification of a Heterotrophic Nitrifying Bacterium and Its Mutation Breeding by UV-irradiation

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