Cloning and Protein Sequence Analysis of CHS Gene Family in Fusarium  oxysporumf.sp. cubense Race 4

Abstract

Abstract: In order to know the characteristics of chitin synthase genes in Fusarium oxysporum f. sp. cubenes race 4 and lay an information foundation for further study of their functions, the degenerate primers were designed according to the sequence of several filamentous fungi’s CHS genes. The FOC4 CHS genes were amplified by PCR and RT-PCR methods. Their cDNAs were sequenced and protein sequences were analyzed. The results showed eight CHS genes were identified in FOC4. Through phylogenetic analysis, the FOC4 CHS proteins can be classified into two families. They also can be classified into seven classes through characteristics of the amino acid sequences analysis. The FOC4 CHS amino acid sequences showed high identity with other Fusarium species by homology camparison analysis.

Key words: FOC4, Cchitin synthase, Sequence analysis

Mao Chao, Yang Laying, Dai Qingdong, Huang Junsheng. Cloning and Protein Sequence Analysis of CHS Gene Family in Fusarium oxysporumf.sp. cubense Race 4[J]. Biotechnology Bulletin, 2014, 0(3): 79-85.

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Cloning and Protein Sequence Analysis of CHS Gene Family in Fusarium oxysporumf.sp. cubense Race 4

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