Complete DNA Sequence Analysis of a Cryptic Plasmid Isolated from Weissella confusa

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.029

Abstract

Abstract: A cryptic plasmid isolated from Weissella confusa QJ012，designated as pQJ012，was sequenced and characterized，and it was a 1489 bp circular molecule with a 42.8% G+C content. The result of BLAST showed that nucleotide sequence of pQJ012 was in homology with pJY33（93%）and pKLCA（92%）. ORF1 encoded a 282 amino acid residue Rep protein that was 91% identical to pJY33 and 85% identical to pKLCA in homology. Sequence analysis indicated that plasmid pQJ012 belonged to pT181 family of rolling-circle replication（RCR）. The pQJ012 may be constructed as a promising expression vector.

Key words: Weissella confuse, cryptic plasmid, sequence analysis, replication protein

WANG Hai-juan, DAI Yu-ke, SU Sen-sen, WANG Ying, PAN Qu. Complete DNA Sequence Analysis of a Cryptic Plasmid Isolated from Weissella confusa[J]. Biotechnology Bulletin, 2016, 32(4): 217-221.

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