Isolation and Identification of Pathogenic Aeromonas veronii Biovar Sobria from Monopterus albus

Abstract

Abstract: Large colonies with the same morphology and color were isolated from liver, spleen and kidney of diseased mud eel, Monopterus albus. 5 isolates randomly selected were proved to be the same bacteria by 16S rRNA sequence analysis. Further research of one strain named HS120920 among them was developed. According to morphology, physiological and biochemical characteristics, analysis of their 16S rRNA and gyrB sequences, the strain was identified as Aeromonas veronii biovar sobria, the LD50 was 5.79×10⁷ CFU/mL. The susceptibility test showed that the strain was highly sensitive to 17 drugs such as seven quinolones, five aminoglycosides, but showed low sensitivity or resistance to other 3 drugs.

Key words: Monopterus albus ,Hemorrhagic disease, Aeromonas veronii , 16S, rRNA gene, GyrB gene

Chen Honglian, Jiang He, Hu Wang, Ling Jun, Duan Guoqing. Isolation and Identification of Pathogenic Aeromonas veronii Biovar Sobria from Monopterus albus[J]. Biotechnology Bulletin, 2014, 0(3): 130-136.

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