Effect of the lipA Inactivation on the Cell-bound Lipase Yield of Burkholderia sp. ZYB002

Abstract

Abstract: Burkholderia sp. ZYB002 strain can produce large amount of extracellular lipase and cell-bound lipase. The type of the cell-bound lipase from Burkholderia sp. ZYB002 was qualitatively analyzed, which will help to develop the whole cell lipase in the organic synthesis. Series of potential cell-bound lipase genes were presumed from the known genomic DNA information of Burkholderia cepacia J2315, which was highly homologous to that of Burkholderia sp. ZYB002. The lipA gene from Burkholderia sp. ZYB002 was selected and then inactivated by homologous recombination. The mutant with lipA inactivation was screened and the cell-bound lipase activity from Burkholderia sp. ZYB002-Δ lipA was tested. The cell-bound lipase activity from Burkholderia sp. ZYB002-Δ lipA decreased by 42%.

Key words: Burkholderia, sp. ZYB002 Extracellular lipase A, Cell-bound lipase, Gene knockout, Triparental mating

Shi Shaolei, Lin Hong, Shu Zhengyu, Liu Yanru, Li Xin, Wu Hailong, Huang Jianzhong^{. Effect of the lipA Inactivation on the Cell-bound Lipase Yield of Burkholderia sp. ZYB002[J]. Biotechnology Bulletin, 2014, 0(3): 123-129.}

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