Relative Quantification of mRNA Transcription of TLR3 and TLR5 in Different Tissues of Yak

Abstract

Abstract:

According to the sequences of yak TLR3 and TLR5, the real-time PCR primers were designed to establish a method for the detection of mRNA transcription of TLRs by Real-time quantitative PCR, and the transcription level of TLRs in different tissues of yak was analyzed. The results showed that two genes have different expression pattern and expression level in tissues. The TLR3 mRNA was detected in examined tissues except mammary gland, and it was expressed at a higher level in heart, large intestine, stomach and muscle. The TLR5 mRNA level was higher in heart, lung, large intestine, small intestine, stomach, muscle and ovary. The results indicated that there exists difference in the transcription level of TLRs in tissues, which may be associated with the recognition of pathogens. For the same gene, the mechanism of gene may bring difference in expression level in different tissues.

Key words: Yak, Transcriptional level, TLRs, Relative quantification

Chen Yabing, Lan Daoliang, Lin Baoshan, Huang Cai, Huang Yong, Li Jian. Relative Quantification of mRNA Transcription of TLR3 and TLR5 in Different Tissues of Yak[J]. Biotechnology Bulletin, 2014, 0(8): 89-93.

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