Correlation Analysis Between IRX3 Gene Expression and Intramuscular Fat Deposition in Tibetan Chicken

doi:10.13560/j.cnki.biotech.bull.1985.2018-0048

Abstract

Abstract: The objective of this study is to establish IRX3 gene expression profiles in different tissues and muscle tissues of different developmental stages,and to analyze the correlations between the expression profile of IRX3 gene and the deposition of intramuscular fat in Tibetan chicken. The expression levels of IRX3 gene in different tissues and muscle tissues at different developmental stages were detected by real-time fluorescence quantitative PCR,intramuscular fat contents of the tissues were also measured,and its correlations with the expression level of IRX3 gene were analyzed. The results showed that the expression of IRX3 gene in lung tissue was significantly higher than that in other tissues（P < 0.01）. The relative mRNA levels of IRX3 in the leg and the chest muscle from 210 days-old cock were significantly higher than that of other ages（P < 0.01）,while the relative expression levels of IRX3 mRNA in the chest muscle from one day old hen were significantly higher than that from other ages（P < 0.01）. Correlation analysis showed that expression levels of IRX3 were positively correlated with intramuscular fat content in cock leg muscle（P < 0.01）,while the expression levels were negatively correlated with intramuscular fat content in female chest muscle（P < 0.01）. As conclusion,there is a correlation between IRX3 gene expression and intramuscular fat content,thus it is inferred that IRX3 might regulate intramuscular fat deposition.

Key words: Tibetan chicken, IRX3, tissue expression, developmental expression, IMF

ZHANG Ya-nan, LIN Ya-qiu, XU Qing, XU Ya-ou, HE Qing-hua. Correlation Analysis Between IRX3 Gene Expression and Intramuscular Fat Deposition in Tibetan Chicken[J]. Biotechnology Bulletin, 2018, 34(9): 219-223.

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