Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (11): 97-106.

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Analysis on Genetic Diversity of Hainan Upland Rice Local Varietiesand Establishment of Molecular ID with SRAP Markers

Yao Yufei1,Wang Ying1,2,Zhuang Nansheng1,2,Gao Heqiong1   

  1. 1. Agriculture College of Hainan University,Haikou 570228;2. Key Laboratory of Protection and Development Utilization of Tropical Grop Germplasm Resources(Hainan University),Ministry of Education,Haikou 570228
  • Received:2014-03-27 Online:2014-11-07 Published:2014-11-07

Abstract: SRAP markers were used to analyze the genetic relationships of 28 upland rice local varieties from Hainan province. Forty-six primer combinations with abundant polymorphism were selected from 255 primer combinations, and 18 primer combinations were picked to amplify the 28 material. A total of 180 bands were scored, and 160(87.75%)out of them were polymorphic, with an average of 10 bands and 8.9 polymorphic bands for each primer combination. Cluster analysis using UPMGA method showed that the genetic similarity coefficient ranged from 0.687 0 to 0.921 2 among the test upland rice varieties. The tested materials were classified into two clusters at the similarity coefficient of 0.687 0, and cluster I included Hainan shanlan upland rice;the cluster Ⅱ involved upland rice poya and varieties developed by our team. The clustering result of Hainan upland rice varieties showed close genetic relationship among Shanlan upland rice varieties. The Hainan upland rice poya might has some genetic similarities with nonglutinous rice germplasms. In addition, we established the molecular identity card of 28 Hainan upland rice varieties with SRAP markers. All these results provided the basis for the Shanlan upland rice heredity relationship and the Shanlan upland rice germplasm resources’ appraisal and the new variety’s breeding.

Key words: Upland rice , Shanlan upland rice , SRAP markers, Genetic diversity, Molecular identity card