Functions of Potato Tuber Formation-related Gene STI-LIKE in Phenotype of Arabidopsis thaliana|

Abstract

Abstract: A series of gene’s expression and silencing were involved in potato tuber formation. The full length of potato STI-LIKE gene was cloned by using the RT-PCR（reverse transcription PCR）method from the potato variety “Atlantic”. The expression of STI-LIKE gene in all potato organs were analyses by real time PCR analysis. The results showed that STI-LIKE gene expressed in all potato organs. The bioinformatics analysis indicated that STI-LIKE protein contains three TPR domains, and the STI-LIKE gene was homologous recombination in higher plants. The expression vector（pBI121）and interference vectors（pHANNIBAL and pART27）were constructed and transferred to Arabidopsis thaliana. The STI-LIKE protein expression level was examined by Western blot with specific antibody. The results indicated that STI-LIKE gene might be associated with the growth and development of seedling of Arabidopsis.

Key words: Potato , STI-LIKE gene TPR, domain , Phenotype

Ping Haitao,Wang Dongxia,Zhou Xiaojie. Functions of Potato Tuber Formation-related Gene STI-LIKE in Phenotype of Arabidopsis thaliana|[J]. Biotechnology Bulletin, 2014, 0(11): 119-124.

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Functions of Potato Tuber Formation-related Gene STI-LIKE in Phenotype of Arabidopsis thaliana|

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[1]	LIU Wen-jin, MA Rui, LIU Sheng-yan, YANG Jiang-wei, ZHANG Ning, SI Huai-jun. Cloning of StCIPK11 Gene and Analysis of Its Response to Drought Stress in Solanum tuberosum [J]. Biotechnology Bulletin, 2023, 39(9): 147-155.
[2]	WANG Gui-fang, YAO Yuan-tao, XU Hai-feng, XIANG Kun, LIANG Jia-hui, ZHANG Shu-hui, WANG Wen-ru, ZHANG Ming-juan, ZHANG Mei-yong, CHEN Xin. The Gene JrSnRK1α1.1 of Walnut Regulates Seed Oil Synthesis and Accumulation [J]. Biotechnology Bulletin, 2023, 39(9): 183-191.
[3]	XU Jing, ZHU Hong-lin, LIN Yan-hui, TANG Li-qiong, TANG Qing-jie, WANG Xiao-ning. Cloning of IbHQT1 Promoter and Identification of Upstream Regulatory Factors in Sweet Potato [J]. Biotechnology Bulletin, 2023, 39(8): 213-219.
[4]	ZHANG Dao-lei, GAN Yu-jun, LE Liang, PU Li. Epigenetic Regulation of Yield-related Traits in Maize and Epibreeding [J]. Biotechnology Bulletin, 2023, 39(8): 31-42.
[5]	ZHANG Yong, XU Tian-jun, LYU Tian-fang, XING Jin-feng, LIU Hong-wei, CAI Wan-tao, LIU Yue-e, ZHAO Jiu-ran, WANG Rong-huan. Effects of Planting Density on the Stem Quality and Root Phenotypic Characters of Summer Sowing Maize [J]. Biotechnology Bulletin, 2023, 39(8): 70-79.
[6]	YANG Jun-zhao, ZHANG Xin-rui, ZHAO Guo-zhu, ZHENG Fei. Structure and Function Analysis of Novel GH5 Multi-domain Cellulase [J]. Biotechnology Bulletin, 2023, 39(4): 71-80.
[7]	TAO Na, LI Mao-xing, GUO Hua-chun. Optimization of Sweet Potato Genetic Transformation System Mediated by Agrobacterium rhizogenes [J]. Biotechnology Bulletin, 2023, 39(10): 175-183.
[8]	LUO Hao-tian, WANG Long, WANG Yu-qian, WANG Yue, LI Jia-zhen, YANG Meng-ke, ZHANG Jie, DENG Xin, WANG Hong-yan. Genome-wide Identification and Expression Analysis of the RNAi-related Gene Families in Setaria viridis [J]. Biotechnology Bulletin, 2023, 39(1): 175-186.
[9]	YU Guo-hong, LIU Peng-cheng, LI Lei, LI Ming-zhe, CUI Hai-ying, HAO Hong-bo, GUO An-qiang. Physiological Responses of Potato in Different Genotypes to Drought Stress [J]. Biotechnology Bulletin, 2022, 38(5): 56-63.
[10]	LI Zhi-kang, LIU Chen-xue-xuan, TAN Chu-min, XIONG Sheng, XIE Qiu-ling. Roles and Functions of MFG-E8 as Carrier Proteins in Exosomes [J]. Biotechnology Bulletin, 2022, 38(4): 288-294.
[11]	GAO Bo, MA Juan, LI Xiu-hua, LI Jiao-sheng, WANG Rong-yan, CHEN Shu-long. Cloning and Functional Analysis of Dd-mel-26 Gene from Ditylenchus destructor [J]. Biotechnology Bulletin, 2021, 37(7): 107-117.
[12]	FAN Yu-chen, LU Yao, LIU Xiang-nan, ZHAO Bo. Construction of Mutants Swapping Ubiquitin E3 Ligase CHIP and E4B U-box Domain and Verification of Ubiquitination Activity [J]. Biotechnology Bulletin, 2021, 37(12): 191-197.
[13]	ZHENG Ying-zhuan, LÜ Yan, YANG Dong-xu, LI Guo-wei, WANG Hong-yang, LI Can-hui. Study on the Identification of Potato Ploidy Using Flow Cytometry Based on Liquid Nitrogen Grinding Method [J]. Biotechnology Bulletin, 2021, 37(1): 282-288.
[14]	LI Pei-gen, YAO Ya-qian, SONG Ji-xiang, WANG Tian-qi, ZHOU Bo, WANG Bing, LIN Rong-shan. Isolation and Identification of IAA-producing Bacillus sp on Potato Rhizosphere and Its Growth-promoting Effect [J]. Biotechnology Bulletin, 2020, 36(9): 109-116.
[15]	LI Jing-shu, ZHAO Jia. Optimization of Fermentation Conditions of Biocontrol Bacterium ML-3 Antifungal Protein and Its Application [J]. Biotechnology Bulletin, 2020, 36(6): 83-92.