Isolation and Identification of IAA-producing Bacillus sp on Potato Rhizosphere and Its Growth-promoting Effect

doi:10.13560/j.cnki.biotech.bull.1985.2019-1203

Abstract

Abstract: Eleven Bacillus strains that have promising ability of producing IAA were isolated from the potato rhizosphere soil in Heilongjiang. The strain HL379 with high yield was identified by morphology,Biolog and 16S rDNA sequence analysis. The pot experiments of growing Arabidopsis thaliana and potato were carried out to study its growth promotion function. The strain was identified as Bacillus aryabhattai,and the yield of IAA was 47.8 mg/L. Growth experiments in the pot showed that potato plant height,fresh weight,dry weight,and root length increased by 13%,32%,107% and 20.8%,respectively,and potato yield increased by 38%,meaning that it had a significant promotion effect on potato growth. It reveals that HL379 has certain application potential in plant growth promotion.

Key words: potato, indole acetic acid, growth promotion function, plant rhizosphere bacteria

LI Pei-gen, YAO Ya-qian, SONG Ji-xiang, WANG Tian-qi, ZHOU Bo, WANG Bing, LIN Rong-shan. Isolation and Identification of IAA-producing Bacillus sp on Potato Rhizosphere and Its Growth-promoting Effect[J]. Biotechnology Bulletin, 2020, 36(9): 109-116.

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