Isolation, Identification and Enzymatic Characterization of a Cellulase-Producing Bacteria

Abstract

Abstract: A cellulase-producing bacteria strain JJ-3 with high activity was isolated from the soil containing rotted leaves, which was identified as Klebsiella oxytoca based on the 16S rRNA sequence analysis. The optimal enzyme-producing conditions of strain JJ-3 was investigated and was as follows：filter paper as carbon source, peptone as nitrogen source, pH8.0, three-day fermentation. And the fermentation culture had higher FPase activity under neutral and alkaline conditions, with 118.7 U/mL（pH7.0）, 167.8 U/mL（pH8.0）, 120 U/mL（pH9.0）. The results of enzymatic characterization showed that the optimal pH and temperature for enzyme reaction was 7.0 and 40℃, respectively. FPase was sensitive to temperature, while it kept good stability during the pH7.0 to 8.0. This study suggested that the cellulase from strain JJ-3 meets the request of neutral and alkaline cellulase.

Key words: Cellulase , Klebsiella oxytoca , Enzyme-producing conditions , Enzymatic characterization

Jia Bohan ,Zhou Wei, Zhao Luodi ,Yang Pu ,Gou Min. Isolation, Identification and Enzymatic Characterization of a Cellulase-Producing Bacteria[J]. Biotechnology Bulletin, 2014, 0(11): 187-192.

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