Preliminary Application of PCR-DGGE to Analyzing Microbial Diversity of Ulva lactuca L. and Dictyota dichotoma

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.012

Abstract

Abstract: To study the microbial diversity of Ulva lactuca L. and Dictyota dichotoma. As a new DNA fingerprinting technique, denaturing gradient gel electrophoresis(DGGE)can be used to analyze the microbial diversity in different environmental samples. Ulva lactuca L. and Dictyota dichotoma will be studied with respect to microbial diversity by applying cultivation-independent molecular methods DGGE. The results showed, the profile of DGGE showed that Ulva lactuca L. and Dictyota dichotoma had different bands. The structural diversity of the microbial community was examined by the Shannon index of general diversity H. Quantity one analysis showed that, V3 region of 16S rRNA gene was isolated from 25 DNA fragments of Ulva lactuca L. and 16 of Dictyota dichotoma. DGGE similarity and UPGMA analysis showed that ：the similarity of Ulva lactuca L. was higher than the Dictyota dichotoma(P<0.05).. Shannon indexes of two kinds of algae all showed Ulva lactuca L. were significantly higher than the Dictyota dichotoma(P<0.05). The algae microbial diversity of Ulva lactuca L. was richer than Dictyota dichotoma.

Key words: PCR-DGGE Ulva lactuca L., Dictyota dichotoma, Algae microorganisms, Microbial diversity

Feng Xuezhen, Wu Shanguang, Lu Yuan. Preliminary Application of PCR-DGGE to Analyzing Microbial Diversity of Ulva lactuca L. and Dictyota dichotoma[J]. Biotechnology Bulletin, 2014, 0(12): 73-77.

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