The Isolation of Beta-amylase-producing Bacteria and Cloning， Expression of Beta-amylase Gene in Escherichia coli

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.027

Abstract

Abstract: A bacterium which can degrade starch was isolate from the soil near the starch factory, was identified as Bacillus cereus by means of morphology and physiological-chemical characterization as well as 16S rDNA sequencing. Beta-amylase gene was cloned from the strain by PCR, and gene sequence was analyzed, which can encode a signal peptide which contains about 30 amino acids. The beta-amylase gene was cloned and expressed in Escherichia coli BL21(DE3). The results showed that recombinant bacteria can produce beta-amylase, and enzymatic activity increased by 53.9% compared with the parent.

Key words: Beta-amylase, Bacillus cereus, Isolate clone, Expression

Li Meng, Chen Lifei, Yang Jianlou, Ma Chunling. The Isolation of Beta-amylase-producing Bacteria and Cloning， Expression of Beta-amylase Gene in Escherichia coli[J]. Biotechnology Bulletin, 2014, 0(12): 161-167.

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