Application Progress of Aptamers in the Detection of Food-borne Pathogenic Bacteria

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.006

Abstract

Abstract: Aptamers are artificial DNA/RNA oligonucleotides binding target ligands with high affinities，and they are selected from the libraries of single-strand nucleic acid random sequence in vitro by SELEX. Compared with the traditional methods，application of aptamers has the obvious advantages of high specificity，chemical stability and simplicity of modification in the detection of food-borne pathogenic bacteria，thus it draws attentions and applications in this field. However，the combination principle of aptamers to the target has not been understood clearly yet，and summaries on the selection of aptamers against food-borne pathogenic bacteria and its application are still deficient. Combined with the characteristics of food-borne pathogenic bacteria，this article describes the selection characteristics of aptamers against them，and the latest progress in the detection field. Finally，the existing problems and development trend are proposed.

Key words: aptamer, food-borne pathogens, biosensor, detection

XIE Pei-yan ,ZHU Long-jiao, XU Wen-tao，. Application Progress of Aptamers in the Detection of Food-borne Pathogenic Bacteria[J]. Biotechnology Bulletin, 2016, 32(4): 48-62.

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