Cloning and Bioinformatic Analysis of γ-Actin Gene（Bbgγ-Actin） from Bufo bufo gargarizans

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.021

Abstract

Abstract: In order to obtain the full-length of actin’s sequence in Bufo bufo gargarizans,the total RNA was extracted from the parotid gland of B. bufo gargarizans,and high-throughput sequencing of transcriptome was used to obtain the full-length cDNA of actin （BbgActin）in B. gargarizansas 2 055 bp. The sequence of ORF（open reading frame）was verified by RT-PCR and sequencing. Sequence analysis showed that the ORF was 1 131 bp encoding for a polypeptide of 376 amino acid residues. Homologous alignment by software BLAST P revealed that it shared over 89% of nucleotide identities and over 90% of amino acid identities with actins from other animals in GenBank. Phylogenetic analysis showed that BbgActin clustered in a class with γ-Actin and highly conserved during evolution. In conclusion,it is the first time to acquire the full-length sequence of γ-Actin cDNA in B. bufo gargarizans,which lays a foundation for further studies on the role of gene Bbgγ-Actin on the life activities of B. bufo gargarizans.

Key words: Bufo bufo gargarizans, γ, -Actin, full-length of cDNA, system evolution

LIU Yan-fang^{1, 2}, WANG Qiu-shuang^{1, 2}, LING Hong^{1, 2}, LIU Dong-chun^{1, 2}, DAI Ying-hui¹, WANG Dong^{1, 2}. Cloning and Bioinformatic Analysis of γ-Actin Gene（Bbgγ-Actin） from Bufo bufo gargarizans[J]. Biotechnology Bulletin, 2016, 32(9): 156-164.

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