Role of Long Non-coding RNA AK043773 in Adipocyte Differentiation

doi:10.13560/j.cnki.biotech.bull.1985.2017-0556

Abstract

Abstract: This work is to investigate the expression changes and regulating role of long non-coding RNA（lncRNA）AK043773 in adipocyte differentiation. First，pre-adipocyte cells in mice were induced for adipogenesis. Then，the expressions of adipogenic differentiation regulating gene（KLF7）and AK043773 were measured by real-time quantitative PCR（RT-qPCR）. Next，the overexpression vector for AK043773 was constructed for detecting its effects on adipogenic differentiation. According to search in UCSC database，the locus of AK043773 was in the 3'-end downstream of KLF7’s exon 4. The expressions of KLF7 and AK043773 were down-regulated coordinately in adipogenesis-induced and differentiated bone marrow stromal cell line ST2 and pre-adipocyte cells 3T3-L1. Furthermore，the overexpression vector pCDNA3.1-AK043773 was constructed and transfected into ST2 cells，which significantly enhanced the expression of AK043773，but inhibited the adipogenesis in ST2 cells by oil red O staining and OD520nm absorbance test after the induction of adipocyte differentiation. All of results indicate that AK043773 and KLF7 coordinately down-regulate in adipocyte differentiation，and increasing the expression of AK043773 remarkably suppresses the differentiation of adipocyte.

Key words: lncRNA, AK043773, adipocyte differentiation, KLF7

ZHANG Juan-juan, QIAO Ling, ZHU En-dong. Role of Long Non-coding RNA AK043773 in Adipocyte Differentiation[J]. Biotechnology Bulletin, 2017, 33(12): 156-161.

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