Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (3): 31-38.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0814

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Research Progress on the Testing Technologies for Composition in Genetically Modified Products

WANG Hao-qian1, CHEN Rui2, LI Xia-ying1, WANG Meng-yu3, LIU Peng-cheng1   

  1. 1. Development Center of Science and Technology,the Ministry of Agriculture,Beijing 100176;
    2. Tianjin Agricultural Quality Standard and Detection Technology Research Institute,Tianjin 300192;
    3. College of Plant Protection,China Agriculture University,Beijing 100193
  • Received:2017-09-26 Online:2018-03-20 Published:2018-04-10

Abstract: With the large-scale commercialized planting of GM(Genetically Modified)crops and the constant development of GM technologies in recent years,GM products with disease resistance,insect resistance,herbicide tolerance,stress resistant,high yield and fine quality have growingly increased and also widely been applied. GM products brought convenience and economic benefits to people while also brought security issues. To strengthen the management of GM products,the development of testing technologies for GMOs were especially critical. At present,the testing technologies for GMOs and their derived products are mainly classified into two categories:one is based on exogenous nucleic acid,mainly including conventional PCR,quantitative PCR,isothermal amplification technology,gene chip technology and so on;the other is based on exogenous protein,mainly including ELISA,Western blot,test strip technology and so on. Each testing technology has its advantages and disadvantages and the scope of application,in real testing process we should select the most effective technology or combination of them for meeting the purpose of testing,while based on the requirements of testing as well as the types and characteristics of GM products. In this paper,we briefly reviewed the principles,advantages and disadvantages and research progress of each testing technology,meanwhile summarized and prospected the development direction of the testing technologies for GMOs,aiming at promoting testing technologies for GMOs in China to better adapt to the current development of transgenic area.

Key words: genetically modified organisms, testing technology, nucleic acid, protein