Research Progress of CRISPR System and Its Application in Mice

doi:10.13560/j.cnki.biotech.bull.1985.2017-0530

Abstract

Abstract: As a gene editing tool,CRISPR system has been in rapid development since the first application in mammal in 2013, and iIt has been widely used in the field of molecular biology with its simple design,short time consuming,and high efficiency advantages. So far,its applications have far exceeded ZFNs and TALEN technology. With the continuous exploration of varied techniques from the CRISPR system,mice as a basic experimental animal play an important role in the application of CRISPR system. At present,the application tools developed from CRISPR system have been successfully applied to a variety of cells cultured in vitro. The technology for producing animal models by editing embryonic cells is also maturing,and it has its unique advantages in the treatment of diseases,especially in the treatment of hereditary diseases. And its scope of application is still expanding,and the mode of application is also in constant innovation. This paper mainly introduces the structure and mechanism of CRISPR system;also describes several major CRISPR application tools acting on DNA for gene knockin,knockout,gene silencing,transcriptional regulation,etc.,discuss several improvement measures focusing on its major deficiency,i.e.,the off-target effects,and its important role in the establishment of mouse disease models and treatment of diseases.

Key words: CRISPR/Cas9 system, gene regulation, off-target effects, mice

DONG Wei-peng, WANG Jun-shi, ZHANG Shao-hua, YAN Jiong. Research Progress of CRISPR System and Its Application in Mice[J]. Biotechnology Bulletin, 2018, 34(5): 57-63.

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