Application of Male Sterility Molecular Markers in Identification of Onion Haploid

doi:10.13560/j.cnki.biotech.bull.1985.2019-0489

Abstract

Abstract: In order to effectively identify and utilize onion haploid,the unfertilized flower buds of three onion hybrid cultivars were used as explants to induce regenerated plants through in vitro gynogenesis. Flow cytometry was used to identify ploidy of regenerated plants,and DNF-566,RNS-357 and AcSKP1 markers were used to detect homozygosity of the regenerated plants. The results showed that the genotypes of plants regenerated from ‘ATON’ and ‘Earth’ were homozygous at Ms locus. Of the sixteen regenerated plants of ‘TABAO’,thirteen were homozygous at Ms locus and three were heterozygous. These results suggest that homozygous plants are derived from megaspore mother cells,and heterozygous plants may be diploid from ovary wall or other somatic cells. Seven diploids were obtained,of which four were doubled-haploid. The doubled-haploid can be further used as parent material for hybrid breeding. The results further show that the molecular markers of Ms locus in onion can be used as an effective method to analyze the homology between haploid regenerated plants and their donor hybrids. Combining with flow cytometry,the double haploid could be identified quickly and accurately.

Key words: onion（Allium cepa L.）, cytoplasmic male sterility, molecular marker, haploid

YANG Yan-yan, HUO Yu-meng, WU Xiong, LIU Bing-jiang. Application of Male Sterility Molecular Markers in Identification of Onion Haploid[J]. Biotechnology Bulletin, 2019, 35(12): 169-174.

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