Advances in Restorer Genes for Fertility on Cytoplasmic Male Sterility in Major Crops

doi:10.13560/j.cnki.biotech.bull.1985.2019-0900

Abstract

Abstract: Heterosis is one of the main ways that can improve the crop yield,stress resistance and quality. The sterile/recovery（CMS/Rf）system is widely used in crop heterosis. Studying the recovery mechanism of cytoplasmic male sterile lines is an important basis of molecular genetic in the selection of the ‘three lines’ breeding program. Currently,different types of sterile lines have been created in major crops. Aiding of molecular technology,genomics and sequencing technology,many fertility-restoring genes have been located,and some of them have been cloned and functionally identified. This paper systematically summarizes the genetic model and molecular marker localization,cloning and application of CMS/Rf system in hybrid breeding in the main crops. It is aimed that this paper may provide ideas for molecular marker assisted selection,transgenic or gene editing methods to create new restorer lines in the future.

Key words: cytoplasmic male sterility, restorer gene, genetic model, molecular marker, cloning

ZHAO Guo-long, LIN Chun-jing, JIN Dong-chun, ZHANG Chun-bao. Advances in Restorer Genes for Fertility on Cytoplasmic Male Sterility in Major Crops[J]. Biotechnology Bulletin, 2020, 36(1): 116-125.

References

[1] 卢庆善, 孙毅, 华泽田. 农作物杂种优势[M]. 北京:中国农业科技出版社, 2003.
[2] 石子, 宋伟, 赵久然. 雄性不育在作物杂种优势中的应用途径分析[J]. 中国生物工程杂志, 2018, 38(1):126-134.
[3] Luo X, Ma CZ, Yi B, et al.Genetic distance revealed by genomic single nucleotide polymorphisms and their relationships with harvest index heterotic traits in rapeseed(Brassica napus L.)[J]. Euphytica, 2016, 209(1):1-7.
[4] Lee YP, Cho Y, Kim S.A high-resolution linkage map of the Rfd1, a restorer-of-fertility locus for cytoplasmic male sterility in radish(Raphanus sativus L.)produced by a combination of bulked segregant analysis and RNA-Seq[J]. Theoretical and Applied Genetics, 2014, 127(10):2243-2252.
[5] 汤复跃, 周立人, 程潇, 等. 大豆M型细胞质雄性不育恢复基因SSR标记初步定位[J]. 大豆科学, 2008(3):383-386.
[6] 任良真. 大豆细胞质雄性不育恢复基因的SSR标记[D]. 长春:吉林大学, 2012.
[7] 焦东燕. 大豆杂种优势及细胞质雄性不育恢复基因的定位[D]. 石家庄:河北师范大学, 2011.
[8] 郑瑞丰, 夏胜平, 陈立云. 水稻野败型恢复系恢复基因的遗传及等位性分析[J]. 湖南农业科学, 2010(23):1-5.
[9] 景润春, 何予卿, 黄青阳. 水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析[J]. 中国农业科学, 2000, 33(2):10-15.
[10] Cai J, Liao QP, Dai ZJ, et al.Allelic differentiations and effects of the Rf3 and Rf4 genes on fertility restoration in rice with wild abortive cytoplasmic male sterility[J]. Biologia Plantarum, 2013, 57(2):274-280.
[11] Liu XQ, Xu X, Tan YP.Inheritance and molecular mapping of two fertility-restoring loci for Honglian gametophytic cytoplasmic male sterility in rice[J]. Mol Genet Genomics, 2004, 271(5):586-594.
[12] 李广贤, 姚方印, 庄杰云, 等. 水稻细胞质雄性不育的育性遗传及恢复基因的定位研究进展[J]. 杂交水稻, 2006(3):1-6.
[13] Wise RP, Schnable PS.Mapping complementary genes in maize:positioning the rf1 and rf2 nuclear-fertility restorer loci of Texas(T)cytoplasm relative to RFLP and visible markers[J]. Theoretical and Applied Genetics, 1994, 88(6):785-795.
[14] Xu XB, Liu ZX, Zhang DF.Isolation and analysis of rice Rf1-orthologus PPR genes co-segregating with Rf3 in maize[J]. Plant Mol Biol Rep, 2009, 27(4):511-517.
[15] Zhang ZF, Wang Y, Zheng YL.AFLP and PCR-based markers linked to Rf3, a fertility restorer gene for S cytoplasmic male sterility in maize[J]. Mol Gen Genomics, 2006, 276(2):162-169.
[16] 邵可可. 玉米C型胞质雄性不育育性恢复主基因Rf4的精细定位与图位克隆[D]. 郑州:河南农业大学, 2011.
[17] 孙文鑫. 小麦K型细胞质雄性不育育性恢复基因的标记定位和效应分析[D]. 郑州:河南农业大学, 2011.
[18] 李志宽, 祝万万, 马守才, 等. 小麦T型细胞质雄性不育恢复基因Rf1和Rf4的SSR标记分析[J]. 农业生物技术学报, 2014, 22(9):1114-1122.
[19] 石运庆, 牟秋焕, 李鹏, 等. V型小麦细胞质雄性不育系育性恢复基因的SSR分子标记分析[J]. 山东农业科学, 2005(3):3-5.
[20] 赵丽梅, 王玉民, 孙寰, 等. 大豆细胞质雄性不育恢复基因SSR标记[J]. 大豆科学, 2007(6):835-839.
[21] Wang YM, Zhao LM, Wang X, et al.Molecular mapping of a fertility restorer gene for cytoplasmic male sterility in soybean[J]. Plant Breeding, 2010, 129(1):9-12.
[22] Dong DK, Li Z, Yuan FJ, et al. Inheritance and fine mapping of a restorer-of-fertility gene for the cytoplasmic male sterility in soybean[J]. Plant Science, 2012, 188-189:36-40.
[23] Wang DG, Zhang L, Li JK, et al.The restorer gene for soybean M-type cytoplasmic male sterility, Rf-m, is located in a PPR gene-rich region on chromosome 16[J]. Plant Breeding, 2016, 135(3):342-348.
[24] 董建生, 杨守萍, 喻德跃, 等. 大豆质核互作雄性不育系NJCMS2A的育性恢复性遗传和育性恢复基因的SSR标记[J]. 大豆科学, 2008(2):181-185.
[25] 刘利彩, 郭立平, 戚廷香, 等. 棉花细胞质雄性不育恢复基因Rf₁候选区域SSCP标记开发与候选基因表达分析[J]. 棉花学报, 2018, 30(1):12-20.
[26] Wu J, Zhang M, Zhang X, et al.Development of InDel markers for the restorer gene Rf1 and assessment of their utility for marker-assisted selection in cotton[J]. Euphytica, 2017, 213(11):2-8.
[27] 曹秀霞, 吴建勇, 邢朝柱, 等. 棉花细胞质雄性不育育性恢复基因的定位及分子标记辅助育种研究进展[J]. 中国农学通报, 2012, 28(6):8-13.
[28] Wang F, Stewart JM, Zhang J.Molecular markers linked to the Rf2 fertility restorer gene in cotton[J]. Genome, 2007, 50(9):818-824.
[29] 郭秀娟, 孙万仓, 杨建春. 甘蓝型油菜细胞质雄性不育系L04-05A恢复基因的SSR标记[J]. 山西农业科学, 2015, 43(7):788-791.
[30] Liu Z, Liu PW, Long FR, et al.Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed(Brassica napus L.)[J]. Theor Appl Genet, 2012, 125(4):773-779.
[31] Brown GG, Formanova N, Jin H, et al.The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats[J]. The Plant Journal, 2003, 35(2):262-272.
[32] 易聪聪. 芥菜型油菜MOR CMS恢复基因的图谱定位[D]. 武汉:华中农业大学, 2013.
[33] 官春云, 尹明智. 利用SSR标记定位油菜细胞质雄性不育系1193A的恢复基因[J]. 植物遗传资源学报, 2015, 16(2):364-367.
[34] Wei C, Wang H, Heng S, et al.Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus[J]. Theoretical and Applied Genetics, 2019, 6(4):1-15.
[35] Tang HW, Xie YG, Liu YG, et al.Advances in understanding the molecular mechanisms of cytoplasmic male sterility and restoration in rice[J]. Plant Reproduction, 2017, 30(4):179-184.
[36] Bharaj TS, Virmani SS, Khush GS.Chromosomal location of fertility restoring genes for wild abortive cytoplasmic male sterility using primary trisomics in rice[J]. Euphytica, 1995, 83(3):169-173.
[37] Balaji SP, Srikanth B, Hemanth KV, et al.Fine mapping of Rf3 and Rf4 fertility restorer loci of WA-CMS of rice(Oryza sativa L.)and validation of the developed marker system for identification of restorer lines[J]. Euphytica, 2012, 187(3):421-435.
[38] Zhu JQ, Zhang HY, Liu Z, et al.Mapping the nuclear fertility restorer gene in rice with simple sequence length polymorphism(SSLP)[J]. High Technology Letters, 2004, 10(4):94-96.
[39] Kazama T, Toriyama K.A fertility restorer gene, Rf4, widely used for hybrid rice breeding encodes a pentatricopeptide repeat protein[J]. Rice, 2014, 7(1):1-5.
[40] Tang HW, Luo DP, Zhou DG, et al.The rice restorer Rf4 for wild-abortive cytoplasmic male sterility encodes a mitochondrial-localized PPR protein that functions in reduction of WA352 transcripts[J]. Molecular Plant, 2014, 7(9):1497-1500.
[41] Huang WC, Yu CC, Hu J, et al.Pentatricopeptide-rqjeat family protein Rf6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility[J]. Proc Natl Acad Sci USA[J]. 2015, 112(48):14984-14989.
[42] Hu J, Wang K, Huang W, et al.The rice pentatricopeptide repeat protein RF5 restores fertility in HongLian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162[J]. Plant Cell, 2012, 24(1):109-122.
[43] Komori T, Ohta S, Murai N.Map-based cloning of a fertilityrestorer gene, Rf-1, in rice(Oryz a sativa L.)[J]. Plant J, 2004, 37:315-325.
[44] Wang ZH, Zou YJ, Li XY, et al.Cytoplasmic male sterility of ricewith boro cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing[J]. Plant Cell, 2006, 18(3):676-687.
[45] 苏爱国, 宋伟, 王帅帅, 等. 玉米细胞质雄性不育及其育性恢复基因的研究进展[J]. 中国生物工程杂志, 2018, 38(1):108-114.
[46] Cui XQ, Wise RP, Schnable PS.The rf2 nuclear restore gene of Male-sterile T-cytoplasm maize[J]. Science, 1996, 272(5266):1334-1336.
[47] Kamps TL, Chase CD.RFLP mapping of the maize gametophytic restorer of fertility locus(Rf3)and aberrant pollen transmission of the nonrestoring Rf3 allele[J]. Theor Appl Genet, 1997, 95, 525-531.
[48] 李鹏, 肖森林, 王淑霞, 等. 玉米S型细胞质雄性不育恢复基因Rf3的精细定位及其候选基因预测[J]. 山东农业科学, 2014, 46(8):1-5.
[49] 李文明. 玉米CMS-S育性恢复基因定位分析及EMS突变体筛选[D]. 武汉:华中农业大学, 2018.
[50] 刘伟华, 罗红兵, 邱博. 玉米CMS-C型雄性不育系C543恢复基因的初定位[J]. 湖南农业大学学报:自然科学版, 2016, 42(1):16-19.
[51] 牟碧涛, 赵卓凡, 岳灵, 等. 两份玉米CMS-C恢复系的育性恢复力测定及恢复基因的分子标记定位[J]. 作物学报, 2019, 45(2):225-234.
[52] Liu YM, Zhao ZF, Lu YL.A preliminary identification of Rf*-A619, a novel restorer gene for CMS-C in maize(Zea mays L.)[J]. Peer J, 2016, 4(4):2719.
[53] 孙玲凌. 玉米C型胞质雄性不育恢复相关基因的克隆[D]. 郑州:河南农业大学, 2008.
[54] 詹克慧. 小麦K型细胞质雄性不育系育性恢复机理的研究[D]. 郑州:河南农业大学, 2006.
[55] 关荣霞, 郭小丽, 刘冬成, 等. 小麦T型细胞质雄性不育恢复基因Rf6的ISSR标记分析[J]. 中国农业科学, 2002, 35(11):1297-1301.
[56] Sinha P, Tomar S, Singh VK, et al.Genetic analysis and molecular mapping of a new fertility restorer gene Rf8 for Triticum timopheevi cytoplasm in wheat(Triticum aestivum L.)using SSR markers[J]. Genetica, 2013, 141(10-12):431-441.
[57] 段阳. T型细胞质雄性不育小麦T763A败育的特点与育性恢复基因Rf3的分子作图[D]. 杨凌:西北农林科技大学, 2017.
[58] 桂安胜. 小麦AL型雄性不育系育性恢复基因的遗传分析和定位[D]. 杨凌:西北农林科技大学, 2016.
[59] 孙慧慧, 杨靖, 卫笑, 等. 小麦BNS雄性不育中国春恢复基因的连锁群检测和QTL初步定位[J]. 麦类作物学报, 2016, 36(7):856-865.
[60] 王鹏年. RN型大豆细胞质雄性不育恢复基因的精细定位[D]. 长春:吉林农业大学, 2016.
[61] 陶银. 大豆质核互作雄性不育系NJCMS1A育性恢复基因的SSR标记定位研究[D]. 南京:南京农业大学, 2012.
[62] 李曙光, 赵团结, 盖钧镒. 大豆质核互作雄性不育系NJCMS3A双亲雄性育性基因的SSR标记[J]. 作物学报, 2010, 36(7):1061-1066.
[63] 连世超, 雷梦林, 张瑞军, 等. 大豆细胞质雄性不育恢复基因的标记定位[J]. 山西农业科学, 2016, 44(5):579-582.
[64] 罗莉斯, 李大雄, 张超, 等. 甘蓝型油菜细胞质雄性不育恢复基因的SSR标记及初步定位[J]. 种子, 2018, 37(1):77-79.
[65] Liu Z, Yang ZH, Wang X, et al.A mitochondria-targeted PPR protein restorespol cytoplasmic male sterility by reducing orf224 transcriptlevels in oilseed rape[J]. Mol Plant, 2016, 9(7):1082-1084.
[66] 廖志强, 况晨光, 许丽芳, 等. 中国甘蓝型油菜细胞质雄性不育的主要类型及在育种实践中的应用[J]. 中国农学通报, 2010, 26(3):105-110.
[67] Uyttewaal M, Arnal N, Quadrado M, et al.Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for Ogura cytoplasmic male sterility[J]. Plant Cell, 2008, 20:3331-3345.
[68] 郝建轶, 李云昌, 胡琼, 等. 油菜野芥细胞质雄性不育恢复基因候选片段的克隆[J]. 中国油料作物学报, 2011, 33(5):433-437.
[69] 尤春源, 聂新辉, 雷江荣, 等. 不同来源国家的三套棉花胞质雄性不育恢复基因的分子标记定位[J]. 新疆农业科学, 2013, 50(6):1003-1007.
[70] 杨路明. 棉花细胞质雄性不育恢复基因的图位克隆及PPR基因家族的分析[D]. 南京:南京农业大学, 2009.
[71] 李丹. 棉花细胞质雄性不育恢复基因的图位克隆[D]. 南京:南京农业大学, 2014.
[72] 彭宝, 张春宝, 张伟, 等. “双高”大豆杂交种吉育612创制及高产制种技术研究[J]. 大豆科学, 2019, 38(3):501-503.
[73] 张泽华. 甘蓝型油菜不同细胞质类型特异分子标记的研发及应用[D]. 杨凌:西北农林科技大学, 2009.