Construction of Genetic Linkage Maps for Armeniaca vulgaris×A. sibirica with Sequence-related Amplified Polymorphism and Simple Sequence Repeats

doi:10.13560/j.cnki.biotech.bull.1985.2018-0594

Abstract

Abstract: Sequence-related amplified polymorphism（SRAP）and simple sequence repeats（SSR）were used to construct draft linkage maps using F₁ population（98 individual plants）derived from a cross between Armeniaca vulgaris×A. sibirica “Longwangmao”and “Youyi’”. The molecular linkage maps of both “Longwangmao” and “Youyi” were constructed respectively using Join Map 4.0 software for the linkage analysis. Totally 132 SRAP and 17 SSR markers were obtained,The male map included 8 linkage groups with 53 SRAP and 9 SSR markers covering a total length of 694.8 centimorgans（cM）with an average of 86.58 cM;averagely there were 7.75 markers for each group and the average genetic distance between 2 markers was 11.21 cM. The female map included 8 linkage groups with 79 SRAP and 8 SSR markers and covered a total length of 924.8 cM with an average of 115.6 cM;there were average of 10.87 markers for each group and the average genetic distance between 2 markers was 10.63 cM.

Key words: Armeniaca vulgaris×A. sibirica, molecular markers, genetic linkage map, SRAP, SSR

WANG Xiao-yi, KONG De-jing, AI Peng-fei. Construction of Genetic Linkage Maps for Armeniaca vulgaris×A. sibirica with Sequence-related Amplified Polymorphism and Simple Sequence Repeats[J]. Biotechnology Bulletin, 2018, 34(11): 103-110.

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