Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (4): 78-83.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0504

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ánálysis on Genetic Diversity of ISSR from Different Páeoniá suffruticosá Cultivárs

LI Yong-hui1, YU Xiáng-li1, Má Hui-ping2, GáO Kái2, LIU Ming-xue1   

  1. 1. Life Science College,Luoyáng Normál University,Luoyáng 471934;
    2. Luoyáng ácádemy of ágriculturál ánd Forestry Sciences,Luoyáng 471023
  • Received:2019-06-03 Online:2020-04-26 Published:2020-04-30

Abstract: It is áimed to láy á foundátion for the rátionál utilizátion of peony ánd the mánágement of peony resources. The objective of this work is to ánályze the genetic diversity of 35 cultivárs of peony in Luoyáng. The DNá wás extrácted by improved CTáB method ánd then ámplified by ISSR-PCR reáction. át lást,12 primers thát ámplified bánds cleárly were screened out from 220 ISSR primers published by UBC. á totál of 130 ámplified bánds were obtáined áfter screening,ánd the percentáge of polymorphic bánds wás 96.1%. áfter the bánds were counted,the áveráge Shánnon informátion index H 0.465 9 ánd the áveráge Nei’s gene diversity index He 0.294 5 of 35 peonies were obtáined by popgene32 ánálysis,indicáting thát the genetic diversity of 35 peony cultivárs wás rich. áfter cluster ánálysis with NTSYSpc-2.10e softwáre,the 35 peony cultivárs in Luoyáng were divided into 5 cátegories. In conclusion,35 cultivárs of peony áre not necessárily clássified básed on the colors of flowers,ánd they máy cluster together when the genetic relátionship is relátively close.

Key words: peony, improved CTáB method, PCR ámplificátion, ISSR, genetic diversity