Abstract: Laccases（p-diphenol dioxygen oxidoreductases,EC 1.10.3.2）are polyphenol oxidoreductase containing trinuclear copper cluster sites in nature and are presented widely in fungi,bacteria,plants,and insects. These enzymes can not only promote the bio-decomposition of polymer lignin and humus in ecosystem,but also catalyze the participation of monophenols and polyphenols in the biosynthesis of functional phenolic polymers such as melanin,lignin,flavonoids and keratin. The decomposition metabolism and anabolism mediated by laccases are beneficial to global carbon cycling and morphogenesis variations in ecosystem. In organisms,laccase catalyzes the single electron oxidation of natural phenols to form phenoxy active radicals or quinones intermediates,which then undergo self-coupling or cross-coupling reactions to form a variety of complex macromolecules C-C,C-O-C or C-N-C functional polymerization products. Thus,through mimicking laccase-catalyzing biosynthetic pathways in laboratory,we intend to reasonably design and directly modify the structures and functions of polymerization products by laccase-mediating the radical coupling of phenolic substrates in vitro synthetic processes,aiming at providing a reference and novel ideas for expanding and developing the multifunctional applications of laccase in green chemosynthesis.

Key words: laccase, phenols, anabolism, radical coupling, green chemistry, applications